CPSF2 Antibody

Novus Biologicals | Catalog # NB100-79823

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all CPSF2 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 450 and 500 of human cleavage and polyadenylation specific factor 2, 100kDa using the numbering given in entry NP_059133.1 (GeneID 53981).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CPSF2 Antibody

Western Blot: CPSF2 Antibody [NB100-79823]

Western Blot: CPSF2 Antibody [NB100-79823]

Western Blot: CPSF2 Antibody [NB100-79823] - Detection of CPSF2 from Hela whole cell lysate (5, 15, 50 microgram), 293T (T; 50 microgram) and mouse NIH3T3 (M; 50 microgram). CPSF100 was also immunoprecipitated by rabbit anti-CPSF100 antibodies NB100-79824 and NB100-79825.
Immunohistochemistry-Paraffin: CPSF2 Antibody [NB100-79823]

Immunohistochemistry-Paraffin: CPSF2 Antibody [NB100-79823]

Immunohistochemistry-Paraffin: CPSF2 Antibody [NB100-79823] - Human pancreatic islet cell tumor (left) and mouse teratoma (right). Antibody used at a dilution of 1:200 (1ug/ml).

Applications for CPSF2 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Chip, WB reactivity reported in (PMID: 25274738).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CPSF2

CPSF100/CPSF2 (cleavage and polyadenylation specific factor 2, 100 kDa subunit) is a subunit of the cleavage and polyadenylation specificity factor (CPSF) involved in mammalian mRNA 3'-end processing. CPSF, along with CstF (cleavage and stimulation factor), is critical for the recognition of cis-acting sequence elements on the pre-mRNA that dictate the site of cleavage and subsequent polyadenylation. CPSF exists as a complex of four proteins that includes CPSF160/CPSF1, CPSF100/CPSF2, CPSF73/CPSF3, and CPSF30/CPSF4. CPSF100/CPSF2 and CPSF73/CPSF3 are both members of the metallo-beta-lactamase superfamily of zinc-dependent hydrolases.

Alternate Names

cleavage and polyadenylation specific factor 2, 100kDa, Cleavage and polyadenylation specificity factor 100 kDa subunit, cleavage and polyadenylation specificity factor subunit 2, CPSF 100 kDa subunit, CPSF 100kDa subunit, CPSF100, KIAA1367cleavage and polyadenylation specific factor 2, 100kD subunit

Entrez Gene IDs

53981 (Human); 51786 (Mouse)

Gene Symbol

CPSF2

UniProt

Q9P2I0 (Human)

Additional CPSF2 Products

Product Documents for CPSF2 Antibody

Certificate of Analysis

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Product Specific Notices for CPSF2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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