CPT1B Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-92666

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CPT1B Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 533-772 of human CPT1B (NP_004368.1). SYQVAKALADDVELYCFQFLPFGKGLIKKCRTSPDAFVQIALQLAHFRDRGKFCLTYEASMTRMFREGRTETVRSCTSESTAFVQAMMEGSHTKADLRDLFQKAAKKHQNMYRLAMTGAGIDRHLFCLYLVSKYLGVSSPFLAEVLSEPWRLSTSQIPQSQIRMFDPEQHPNHLGAGGGFGPVADDGYGVSYMIAGENTIFFHISSKFSSSETNAQRFGNHIRKALLDIADLFQVPKAYS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CPT1B Antibody - BSA Free

CPT1B Antibody - BSA Free

Western Blot: CPT1B Antibody - BSA Free [NBP2-92666] -

Western Blot: CPT1B Antibody - BSA Free [NBP2-92666] - Western blot analysis of lysates from Rat skeletal muscle, using CPT1B Rabbit pAb at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.
Immunohistochemistry-Paraffin: CPT1B Antibody - BSA Free [NBP2-92666]

Immunohistochemistry-Paraffin: CPT1B Antibody - BSA Free [NBP2-92666]

Immunohistochemistry-Paraffin: CPT1B Antibody [NBP2-92666] - Paraffin-embedded mouse heart using CPT1B.
Immunohistochemistry-Paraffin: CPT1B Antibody - BSA Free [NBP2-92666]

Immunohistochemistry-Paraffin: CPT1B Antibody - BSA Free [NBP2-92666]

Immunohistochemistry-Paraffin: CPT1B Antibody [NBP2-92666] - Paraffin-embedded human colon using CPT1B.
CPT1B Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CPT1B Antibody - BSA Free [NBP2-92666] -

Immunocytochemistry/ Immunofluorescence: CPT1B Antibody - BSA Free [NBP2-92666] - Immunofluorescence analysis of Mouse heart tissue using CPT1B Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.
CPT1B Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CPT1B Antibody - BSA Free [NBP2-92666] -

Immunocytochemistry/ Immunofluorescence: CPT1B Antibody - BSA Free [NBP2-92666] - Immunofluorescence analysis of Human heart tissue using CPT1B Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.

Applications for CPT1B Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CPT1B

The protein encoded by the CPT1B gene, a member of the carnitine/choline acetyltransferase family, is the rate-controllingenzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the nettransport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variantsencoding different isoforms have been found for this gene, and read-through transcripts are expressed from theupstream locus that include exons from this gene. (provided by RefSeq)

Long Name

Carnitine O-palmitoyltransferase 1, muscle isoform

Alternate Names

CPT1-M, CPTI-M, KIAA1670

Gene Symbol

CPT1B

Additional CPT1B Products

Product Documents for CPT1B Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CPT1B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CPT1B Antibody - BSA Free

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Protocols

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