CRALBP Antibody (JE35-09)

Western Blot: CRALBP Antibody (JE35-09) [NBP3-32215]

Western Blot: CRALBP Antibody (JE35-09) [NBP3-32215] - Western blot analysis of CRALBP on different lysates with Rabbit anti-CRALBP antibody (NBP3-32215) at 1/500 dilution.

Lane 1: Rat eyeball tissue lysate
Lane 2: Mouse eyeball tissue lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 36.5 kDa
Observed band size: 36.5 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32215) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: CRALBP Antibody (JE35-09) [NBP3-32215]

Immunocytochemistry/ Immunofluorescence: CRALBP Antibody (JE35-09) [NBP3-32215] - Immunocytochemistry analysis of B16F1 cells labeling CRALBP with Rabbit anti-CRALBP antibody (NBP3-32215) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CRALBP antibody (NBP3-32215) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Flow Cytometry: CRALBP Antibody (JE35-09) [NBP3-32215]

Flow Cytometry: CRALBP Antibody (JE35-09) [NBP3-32215] - Flow cytometric analysis of B16F1 cells labeling CRALBP.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32215, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).