Creatine Kinase BB Antibody (2ba6)

Novus Biologicals | Catalog # NBP2-59600

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2ba6
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Product Specifications

Immunogen

Human Creatine Kinase BB protein (Uniprot: P12277)

Reactivity Notes

Predicted to react with Chimpanzee, Rhesus Monkey, Canine, Bovine, Mouse, Rat, Chicken, Zebrafish, Frog.

Localization

Cytoplasmic

Specificity

The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. The specificity of this monoclonal antibody to its intended target was validated by HuProt Array containing more than 19,000 full-length, correctly-folded human proteins. Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This monoclonal antibody recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB. It shows minimal reactivity with other human serum proteins.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Human Chromosome Location:14q32


200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-59601)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Creatine Kinase BB Antibody (2ba6)

Protein Array: Creatine Kinase BB Antibody (2ba6) [NBP2-59600]

Protein Array: Creatine Kinase BB Antibody (2ba6) [NBP2-59600]

Protein Array: Creatine Kinase BB Antibody (2ba6) [NBP2-59600] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of Ab to its intended target. A Ab is considered to specific to its intended target, if Ab has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Applications for Creatine Kinase BB Antibody (2ba6)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Frozen

1-2 ug/ml

Protein Array

1:100-1:2000
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Frozen Only): 1-2ug/ml for 30 minutes at RT.
Optimal dilution for a specific application should be determined.

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Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Product Documents for Creatine Kinase BB Antibody (2ba6)

Certificate of Analysis

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Product Specific Notices for Creatine Kinase BB Antibody (2ba6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

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