CRYBA4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32741

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Canine (94%), Guinea Pig (93%), Rat (91%), Rhesus Macaque (96%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CRYBA4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human CRYBA4. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CRYBA4 Antibody - BSA Free

Western Blot: CRYBA4 Antibody [NBP1-32741]

Western Blot: CRYBA4 Antibody [NBP1-32741]

Western Blot: CRYBA4 Antibody [NBP1-32741] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CRYBA4 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: CRYBA4 Antibody [NBP1-32741]

Immunocytochemistry/ Immunofluorescence: CRYBA4 Antibody [NBP1-32741]

Immunocytochemistry/Immunofluorescence: CRYBA4 Antibody [NBP1-32741] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Western Blot: CRYBA4 Antibody [NBP1-32741]

Western Blot: CRYBA4 Antibody [NBP1-32741]

Western Blot: CRYBA4 Antibody [NBP1-32741] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:1000.
Western Blot: CRYBA4 Antibody [NBP1-32741]

Western Blot: CRYBA4 Antibody [NBP1-32741]

Western Blot: CRYBA4 Antibody [NBP1-32741] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CRYBA4 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for CRYBA4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CRYBA4

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, is part of a gene cluster with beta-B1, beta-B2, and beta-B3.

Alternate Names

Beta-A4 crystallin, beta-crystallin A4, crystallin, beta A4, crystallin, beta polypeptide A4, eye lens structural protein, MCOPCT4

Gene Symbol

CRYBA4

Additional CRYBA4 Products

Product Documents for CRYBA4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CRYBA4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for CRYBA4 Antibody - BSA Free

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Protocols

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FAQs

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