Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 18E7

Format

BSA Free
View all CTGF/CCN2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human CTGF/CCN2 (27-349 aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for CTGF/CCN2 Antibody (18E7) - BSA Free

Western Blot: CTGF/CCN2 Antibody (18E7)BSA Free [NBP2-60235]

Western Blot: CTGF/CCN2 Antibody (18E7)BSA Free [NBP2-60235]

Western Blot: CTGF/CCN2 Antibody (18E7) [NBP2-60235] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTGF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate Lane 2.: A549 cell lysate Lane 3.: U87MG cell lysate Lane 4.: A427 cell lysate
Immunocytochemistry/ Immunofluorescence: CTGF/CCN2 Antibody (18E7) - BSA Free [NBP2-60235]

Immunocytochemistry/ Immunofluorescence: CTGF/CCN2 Antibody (18E7) - BSA Free [NBP2-60235]

Immunocytochemistry/Immunofluorescence: CTGF/CCN2 Antibody (18E7) [NBP2-60235] - Analysis of CTGF in Hep3B cells. The cell was stained with ATGA0468 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Applications for CTGF/CCN2 Antibody (18E7) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CTGF/CCN2

Connective Tissue Growth Factor (CTGF) is a 38kDa, cysteine-rich, secreted peptide. CTGF promotes endothelial cell growth, migration, adhesion and survival. and is thus implicated in endothelial cell function and angiogenesis. CTGF is implicated in are embryogenesis, wound healing and regulation of extracellular matrix production, and is required for normal skeletal growth.

CTGF antibodies are useful tools for angiogenesis and cell structure research, and for studies on certian types of cancer.

Long Name

Connective Tissue Growth Factor

Alternate Names

CCN2, CTGRP, Fisp12, HCS24, IGFBP-8, NOV2

Gene Symbol

CCN2

Additional CTGF/CCN2 Products

Product Documents for CTGF/CCN2 Antibody (18E7) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CTGF/CCN2 Antibody (18E7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CTGF/CCN2 Antibody (18E7) - BSA Free

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  • Q: We are looking for a pair of mouse CTGF antibody for ELISA, we prefer no azide and/or glycerol, and in carrier free form (no EDTA, no Tris, no BSA). For the validation, we need approximately 100ug. If it works, we'll order more later.

    A: Unfortunately we do not carry any CTGF antibodies that suit your specifications. We do carry AbSelect antibody purification kits that will remove BSA, Tris and azide from antibody formulations which may be an option for you.

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