Ctip1 Antibody (3M8M3)
Novus Biologicals | Catalog # NBP3-16790
Recombinant Monoclonal Antibody
![Western Blot: Ctip1 Antibody (3M8M3) [NBP3-16790]](https://resources.rndsystems.com/images/products/Ctip1-Antibody-3M8M3-Western-Blot-NBP3-16790-img0002.jpg "Western Blot: Ctip1 Antibody (3M8M3) [NBP3-16790]")
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3M8M3 expressed in HEK293
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Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 401-589 of human Ctip1 (Q9H165). GEKPYKCNLCDHACTQASKLKRHMKTHMHKSSPMTVKSDDGLSTASSPEPGTSDLVGSASSALKSVVAKFKSENDPNLIPENGDEEEEEDDEEEEEEEEEEEEELTESERVDYGFGLSLEAARHHENSSRGAVVGVGDESRALPDVMQGMVLSSMQHFSEAFHQVLGEKHKRGHLAEAEGHRDTCDEDS
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Ctip1 Antibody (3M8M3)
Western Blot: Ctip1 Antibody (3M8M3) [NBP3-16790]
Western Blot: Ctip1 Antibody (3M8M3) [NBP3-16790] - Western blot analysis of extracts of Rat brain, using Ctip1 Rabbit mAb (NBP3-16790) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.![Immunohistochemistry-Paraffin: Ctip1 Antibody (3M8M3) [NBP3-16790]](https://resources.rndsystems.com/images/products/Ctip1-Antibody-3M8M3-Immunohistochemistry-Paraffin-NBP3-16790-img0001.jpg "Immunohistochemistry-Paraffin: Ctip1 Antibody (3M8M3) [NBP3-16790]")
Immunohistochemistry-Paraffin: Ctip1 Antibody (3M8M3) [NBP3-16790]
Immunohistochemistry-Paraffin: Ctip1 Antibody (3M8M3) [NBP3-16790] - Immunohistochemistry of paraffin-embedded human colon carcinoma using Ctip1 Rabbit mAb (NBP3-16790) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western Blot: Ctip1 Antibody (3M8M3) [NBP3-16790] -
Western Blot: Ctip1 Antibody (3M8M3) [NBP3-16790] - Western blot analysis of lysates from wild type(WT) and Ctip1 knockout (KO) 293T(KO) cells, using Ctip1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Applications for Ctip1 Antibody (3M8M3)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Ctip1
Alternate Names
B-cell CLL/lymphoma 11A, B-cell CLL/lymphoma 11A (zinc finger protein), B-cell CLL/lymphoma 11A (zinc finger protein) isoform 2, B-cell lymphoma/leukemia 11A, BCL-11A, BCL11A B-cell CLL/lymphoma 11A (zinc finger protein) isoform 1, BCL11A-S, BCL11A-XL, C2H2-type zinc finger protein, COUP-TF-interacting protein 1, CTIP1ecotropic viral integration site 9, ecotropic viral integration site 9 homolog, Ecotropic viral integration site 9 protein homolog, EVI-9, EVI9FLJ34997, HBFQTL5, KIAA1809BCL11A-L, Zinc finger protein 856, ZNF856FLJ10173
Gene Symbol
BCL11A
Additional Ctip1 Products
Product Documents for Ctip1 Antibody (3M8M3)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Ctip1 Antibody (3M8M3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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