Ctip1 Antibody (BCL11A/123) - BSA Free

Novus Biologicals | Catalog # NBP2-50115

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # BCL11A/123

Format

BSA Free
View all Ctip1 Primary Antibodies »

Product Specifications

Immunogen

C-terminus of extra long isoform of Ctip1, BCL11AXL (aa 637-835)

Specificity

Specifically recognises the extra long form of the protein and not the long or short forms of Ctip1. Not cross reactive with the long form of the BCL11B protein that shares significant sequence identity with the immunogen.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ctip1 Antibody (BCL11A/123) - BSA Free

Western Blot: Ctip1 Antibody (BCL11A/123)BSA Free [NBP2-50115]

Western Blot: Ctip1 Antibody (BCL11A/123)BSA Free [NBP2-50115]

Western Blot: Ctip1 Antibody (BCL11A/123) [NBP2-50115] - Analysis of various lysates of transfectants expressing different BCL11A isoforms and the highly homologous BCL11B to show specificity for BCL11AXL.
Immunocytochemistry/ Immunofluorescence: Ctip1 Antibody (BCL11A/123) - BSA Free [NBP2-50115]

Immunocytochemistry/ Immunofluorescence: Ctip1 Antibody (BCL11A/123) - BSA Free [NBP2-50115]

Immunocytochemistry/Immunofluorescence: Ctip1 Antibody (BCL11A/123) [NBP2-50115] - Analysis of Ctip1 expression in tonsil BCL11AXL is present in B cells (brown) in germinal centres and mantle zones. Double labelling studies show the strongly labelled cells in the interfollicular areas to be CD123-positive pDCs (arrowhead).
Immunohistochemistry: Ctip1 Antibody (BCL11A/123) - BSA Free [NBP2-50115]

Immunohistochemistry: Ctip1 Antibody (BCL11A/123) - BSA Free [NBP2-50115]

Immunohistochemistry: Ctip1 Antibody (BCL11A/123) [NBP2-50115] - BCL11AXL (BCL123a) expression in tonsil BCL11AXL is present in B cells (brown) in germinal centres and mantle zones.
Immunocytochemistry/ Immunofluorescence: Ctip1 Antibody (BCL11A/123) - BSA Free [NBP2-50115]

Immunocytochemistry/ Immunofluorescence: Ctip1 Antibody (BCL11A/123) - BSA Free [NBP2-50115]

Immunocytochemistry/Immunofluorescence: Ctip1 Antibody (BCL11A/123) [NBP2-50115] - BCL11AXL is absent in plasma cells BCL11A+ cells (blue) /CD3+ T cells (red) / p63-positive plasma cells (green)
Immunocytochemistry/ Immunofluorescence: Ctip1 Antibody (BCL11A/123) - BSA Free [NBP2-50115]

Immunocytochemistry/ Immunofluorescence: Ctip1 Antibody (BCL11A/123) - BSA Free [NBP2-50115]

Immunocytochemistry/Immunofluorescence: Ctip1 Antibody (BCL11A/123) [NBP2-50115] - Nuclear staining of COS/ BCL11AXL transfectants but not BCL BCL11AL transfectants using antibody BCL123a.

Applications for Ctip1 Antibody (BCL11A/123) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
IHC: Frozen use neat supernatant. WB: use neat supernatant. Positive control(s): Tonsil (in B cells and plasmcytoid dendritic cells), Neurones and nuclei of some epithelium.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ctip1

The Ctip1 gene encodes a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. The corresponding mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, throug

Alternate Names

B-cell CLL/lymphoma 11A, B-cell CLL/lymphoma 11A (zinc finger protein), B-cell CLL/lymphoma 11A (zinc finger protein) isoform 2, B-cell lymphoma/leukemia 11A, BCL-11A, BCL11A B-cell CLL/lymphoma 11A (zinc finger protein) isoform 1, BCL11A-S, BCL11A-XL, C2H2-type zinc finger protein, COUP-TF-interacting protein 1, CTIP1ecotropic viral integration site 9, ecotropic viral integration site 9 homolog, Ecotropic viral integration site 9 protein homolog, EVI-9, EVI9FLJ34997, HBFQTL5, KIAA1809BCL11A-L, Zinc finger protein 856, ZNF856FLJ10173

Gene Symbol

BCL11A

Additional Ctip1 Products

Product Documents for Ctip1 Antibody (BCL11A/123) - BSA Free

Certificate of Analysis

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Product Specific Notices for Ctip1 Antibody (BCL11A/123) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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