CTRP9/C1qTNF9 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-46834

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DEMWLQVTGGERFNGLFADEDDDTTF

Reactivity Notes

Mouse reactivity reported in the scientific literature (PMID: 30060752).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CTRP9/C1qTNF9 Antibody - BSA Free

Western Blot: CTRP9/C1qTNF9 Antibody [NBP2-46834]

Western Blot: CTRP9/C1qTNF9 Antibody [NBP2-46834]

Western Blot: CTRP9/C1qTNF9 Antibody [NBP2-46834] - Analysis in human cell line MCF-7.
Immunohistochemistry-Paraffin: CTRP9/C1qTNF9 Antibody [NBP2-46834]

Immunohistochemistry-Paraffin: CTRP9/C1qTNF9 Antibody [NBP2-46834]

Immunohistochemistry-Paraffin: CTRP9/C1qTNF9 Antibody [NBP2-46834] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Western Blot: CTRP9/C1qTNF9 Antibody [NBP2-46834]

Western Blot: CTRP9/C1qTNF9 Antibody [NBP2-46834]

CTRP9-C1qTNF9-Antibody-Western-Blot-NBP2-46834-img0005.jpg
Western Blot: CTRP9/C1qTNF9 Antibody [NBP2-46834]

Western Blot: CTRP9/C1qTNF9 Antibody [NBP2-46834]

CTRP9-C1qTNF9-Antibody-Western-Blot-NBP2-46834-img0006.jpg
CTRP9/C1qTNF9 Antibody

Western Blot: CTRP9/C1qTNF9 Antibody [NBP2-46834] -

Western Blot: CTRP9/C1qTNF9 Antibody [NBP2-46834] - Expression of C1q/TNF-related protein 9 (CTRP9) & adiponectin receptor 1 (AdipoR1) after intracerebral hemorrhage (ICH). a Representative western blot images & quantitative analyses of CTRP9 time course after ICH. b Representative western blot images & quantitative analyses of AdipoR1 time course after ICH. Values are expressed as mean ± SD. *p < 0.05, **p < 0.01 vs. sham group. N = 6. c Double immunofluorescence staining for AdipoR1 (red) in microglia (Iba-1, green), neurons (NeuN, green), & astrocytes (GFAP, green) in right basal cortex 24 h after ICH. Scale bar = 50 μm. N = 2. DAPI, 4′,6-diamidino-2-phenylindole; Iba-1, ionized calcium binding adaptor molecule-1; NeuN, neuronal nuclear; GFAP, glial fibrillary acidic protein Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30060752), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CTRP9/C1qTNF9 Antibody

Western Blot: CTRP9/C1qTNF9 Antibody [NBP2-46834] -

Western Blot: CTRP9/C1qTNF9 Antibody [NBP2-46834] - Knockdown of AdipoR1 & p-AMPK abolished improvement in neurological function & anti-inflammatory effects of rCTRP9 at 24 h post-ICH. a Modified Garcia test, b forelimb placement test, c corner turn test, & d representative western blot images & quantitative analyses of CTRP9, AdipoR1, APPL1, p-AMPK/AMPK, p-NF kappa B/NF kappa B, TNF-alpha, & IL-6 at 24 h post-ICH. Values are expressed as mean ± SD. *p < 0.05, **p < 0.01 vs. sham group; #p < 0.05, ##p < 0.01 vs. ICH + vehicle group; &p < 0.05 vs. rCTRP9 + Scr siRNA, & @p < 0.05 vs. rCTRP9 + DMSO group. Scr, scramble; siRNA, small interfering RNA. N = 6 Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30060752), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CTRP9/C1qTNF9 Antibody - BSA Free

Immunohistochemistry: CTRP9/C1qTNF9 Antibody - BSA Free [NBP2-46834] -

CTRP9 expression was diminished within the cardiac tissue of diabetic mice. (A) Illustrative immunohistochemistry images of CTRP9 in two groups. (B) Assessment of CTRP9 in two groups. (C) Illustrative Western blot of CTRP9 in animals. (D) Assessment of CTRP9 in two groups. (E) Illustrative Western blot of CTRP9 in cardiac fibroblasts. (F) Measurement of CTRP9 in two groups. Scale bar = 50 μm. The data are depicted as the mean +/- SEM (n = 5–6). *p < 0.05, ****p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fphar.2024.1407883/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CTRP9/C1qTNF9 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CTRP9/C1qTNF9

C1qTNF9, also known as CTRP9, is one of several Adiponectin/Acrp30 paralogs which comprise the C1q and TNF-related protein family. All family members share a modular organization comprising a short variable region, a collagenous domain, and a C1q-like globular domain. C1qTNF9 is a 40 kDa glycoprotein that contains multiple hydroxylated proline residues in its collagenous region. It circulates as a homotrimer and higher order multimers as well as in heterotrimers with Adiponectin. It is preferentially expressed in adipose tissue and plays a role in glucose homeostasis. Human C1qTNF9 shares 85% amino acid sequence identity with mouse and rat C1qTNF9.

Long Name

C1q and Tumor Necrosis Factor Related Protein 9

Alternate Names

AQL1, C1qTNF9

Entrez Gene IDs

387911 (Human)

Gene Symbol

C1QTNF9

UniProt

Additional CTRP9/C1qTNF9 Products

Product Documents for CTRP9/C1qTNF9 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CTRP9/C1qTNF9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Citations for CTRP9/C1qTNF9 Antibody - BSA Free

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Protocols

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