C1qTNF9, also known as CTRP9, is one of several Adiponectin/Acrp30 paralogs which comprise the C1q and TNF-related protein family. All family members share a modular organization comprising a short variable region, a collagenous domain, and a C1q-like globular domain. C1qTNF9 is a 40 kDa glycoprotein that contains multiple hydroxylated proline residues in its collagenous region. It circulates as a homotrimer and higher order multimers as well as in heterotrimers with Adiponectin. It is preferentially expressed in adipose tissue and plays a role in glucose homeostasis. Human C1qTNF9 shares 85% amino acid sequence identity with mouse and rat C1qTNF9.
CTRP9/C1qTNF9 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-46834
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Rat (92%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DEMWLQVTGGERFNGLFADEDDDTTF
Reactivity Notes
Mouse reactivity reported in the scientific literature (PMID: 30060752).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CTRP9/C1qTNF9 Antibody - BSA Free
Western Blot: CTRP9/C1qTNF9 Antibody [NBP2-46834]
Western Blot: CTRP9/C1qTNF9 Antibody [NBP2-46834] - Analysis in human cell line MCF-7.Immunohistochemistry-Paraffin: CTRP9/C1qTNF9 Antibody [NBP2-46834]
Immunohistochemistry-Paraffin: CTRP9/C1qTNF9 Antibody [NBP2-46834] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.Western Blot: CTRP9/C1qTNF9 Antibody [NBP2-46834]
CTRP9-C1qTNF9-Antibody-Western-Blot-NBP2-46834-img0006.jpgWestern Blot: CTRP9/C1qTNF9 Antibody [NBP2-46834] -
Western Blot: CTRP9/C1qTNF9 Antibody [NBP2-46834] - Expression of C1q/TNF-related protein 9 (CTRP9) & adiponectin receptor 1 (AdipoR1) after intracerebral hemorrhage (ICH). a Representative western blot images & quantitative analyses of CTRP9 time course after ICH. b Representative western blot images & quantitative analyses of AdipoR1 time course after ICH. Values are expressed as mean ± SD. *p < 0.05, **p < 0.01 vs. sham group. N = 6. c Double immunofluorescence staining for AdipoR1 (red) in microglia (Iba-1, green), neurons (NeuN, green), & astrocytes (GFAP, green) in right basal cortex 24 h after ICH. Scale bar = 50 μm. N = 2. DAPI, 4′,6-diamidino-2-phenylindole; Iba-1, ionized calcium binding adaptor molecule-1; NeuN, neuronal nuclear; GFAP, glial fibrillary acidic protein Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30060752), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: CTRP9/C1qTNF9 Antibody [NBP2-46834] -
Western Blot: CTRP9/C1qTNF9 Antibody [NBP2-46834] - Knockdown of AdipoR1 & p-AMPK abolished improvement in neurological function & anti-inflammatory effects of rCTRP9 at 24 h post-ICH. a Modified Garcia test, b forelimb placement test, c corner turn test, & d representative western blot images & quantitative analyses of CTRP9, AdipoR1, APPL1, p-AMPK/AMPK, p-NF kappa B/NF kappa B, TNF-alpha, & IL-6 at 24 h post-ICH. Values are expressed as mean ± SD. *p < 0.05, **p < 0.01 vs. sham group; #p < 0.05, ##p < 0.01 vs. ICH + vehicle group; &p < 0.05 vs. rCTRP9 + Scr siRNA, & @p < 0.05 vs. rCTRP9 + DMSO group. Scr, scramble; siRNA, small interfering RNA. N = 6 Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30060752), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunohistochemistry: CTRP9/C1qTNF9 Antibody - BSA Free [NBP2-46834] -
CTRP9 expression was diminished within the cardiac tissue of diabetic mice. (A) Illustrative immunohistochemistry images of CTRP9 in two groups. (B) Assessment of CTRP9 in two groups. (C) Illustrative Western blot of CTRP9 in animals. (D) Assessment of CTRP9 in two groups. (E) Illustrative Western blot of CTRP9 in cardiac fibroblasts. (F) Measurement of CTRP9 in two groups. Scale bar = 50 μm. The data are depicted as the mean +/- SEM (n = 5–6). *p < 0.05, ****p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fphar.2024.1407883/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for CTRP9/C1qTNF9 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04 - 0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CTRP9/C1qTNF9
Long Name
C1q and Tumor Necrosis Factor Related Protein 9
Alternate Names
AQL1, C1qTNF9
Entrez Gene IDs
387911 (Human)
Gene Symbol
C1QTNF9
UniProt
Additional CTRP9/C1qTNF9 Products
Product Documents for CTRP9/C1qTNF9 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CTRP9/C1qTNF9 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for CTRP9/C1qTNF9 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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