Cultrecoat BME Cell Invasion Optimization Assay, 96-well

Provides three concentrations of BME for invasion assay optimization

Discontinued Product

3484-096-K has been discontinued.
View all Basement Membrane Extracts products.
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Cultrecoat BME Cell Invasion Optimization Assay, 96-well Summary

The CultreCoat Optimization System provides three concentrations of BME for invasion assay optimization.

Why Use Cultrecoat BME Cell Invasion Optimization Assay, 96-well?

The CultreCoat Optimization System is a flexible approach that allows you to choose the desired degree of cell invasion necessary for a specific project. Included in the system are a series of Boyden chambers coated at three distinct concentrations of BME and uncoated wells to compare invasion to basal migration. Relative to uncoated wells, cell invasion decreases as the concentration of the BME coating increases. This system provides an easy approach to determine the ideal coating density that provides the maximum signal-to-noise ratio and dynamic required for your study. Once the desired concentration is determined, researchers may select the BME coated plates with the optimal coating for their study. The system is available in both 96 well and 24 well formats.

Kit Contents

• CultreCoat® 96 Well BME Cell Invasion Optimization Chamber
• Calcein AM
• 25X Cell Wash Buffer
• 10X Cell Dissociation Solution

Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.

Specifications

Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Mouse

Limitations

For research use only. Not for diagnostic use.

Product Datasheets

FAQs

  1. For the 96-well Cultrex Basement Membrane Extract Cell Invasion/Migration Assays, can a partial plate be used, and the rest saved for later?

    • The 96-well microplate chamber does not have removable strips. However, the plate is stable, so unused wells may be used at a later date. The difficult part is to maintain sterility. To prevent contamination, unused wells may be sealed using a sterile sealing film. The BME coating needs to be applied fresh before each invasion assay. The BME and Calcein AM may be divided into working aliquots to limit freeze-thaws and stored at the temperature recommended in the kit booklet, for optimal stability. For maximum flexibility, we would recommend using the 24-well assays, as inserts for the 24-well plate are removable.

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