Cultrecoat BME Cell Invasion Optimization Assay, 96-well
Cultrecoat BME Cell Invasion Optimization Assay, 96-well Summary
Why Use Cultrecoat BME Cell Invasion Optimization Assay, 96-well?
The CultreCoat Optimization System is a flexible approach that allows you to choose the desired degree of cell invasion necessary for a specific project. Included in the system are a series of Boyden chambers coated at three distinct concentrations of BME and uncoated wells to compare invasion to basal migration. Relative to uncoated wells, cell invasion decreases as the concentration of the BME coating increases. This system provides an easy approach to determine the ideal coating density that provides the maximum signal-to-noise ratio and dynamic required for your study. Once the desired concentration is determined, researchers may select the BME coated plates with the optimal coating for their study. The system is available in both 96 well and 24 well formats.
• CultreCoat® 96 Well BME Cell Invasion Optimization Chamber
• Calcein AM
• 25X Cell Wash Buffer
• 10X Cell Dissociation Solution
Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.
For research use only. Not for diagnostic use.
For the 96-well Cultrex Basement Membrane Extract Cell Invasion/Migration Assays, can a partial plate be used, and the rest saved for later?
The 96-well microplate chamber does not have removable strips. However, the plate is stable, so unused wells may be used at a later date. The difficult part is to maintain sterility. To prevent contamination, unused wells may be sealed using a sterile sealing film. The BME coating needs to be applied fresh before each invasion assay. The BME and Calcein AM may be divided into working aliquots to limit freeze-thaws and stored at the temperature recommended in the kit booklet, for optimal stability. For maximum flexibility, we would recommend using the 24-well assays, as inserts for the 24-well plate are removable.
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