Cultrex 3-D Culture Matrix Laminin I

Catalog # Availability Size / Price Qty
3446-005-01
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Cultrex 3-D Culture Matrix Laminin I Summary

Cultrex 3-D Culture Matrix Laminin I is an extracellular matrix hydrogel that directs cells to grow in three dimensions and assemble into organotypic structures in vitro.

Why Use Cultrex 3-D Culture Matrix Laminin I?

Cultrex 3-D Culture Matrix Laminin I is purified from Engelbreth-Holm-Swarm (EHS) tumor and is provided at a high concentration that is capable of polymerizing to form a hydrogel at 37°C. Laminin I is a major component of the basement membrane which is a continuous sheet of specialized extracellular matrix that forms an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that plays an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. Cultrex 3-D Culture Matrix Laminin I is a purified basement membrane protein that has been developed, produced and qualified specifically for use in 3-D culture studies. Cultrex 3-D Culture Matrix Laminin I may also be used as to supplement customized hydrogel or medium formulations for cell culture.

Specifications

Source
Murine Engelbreth-Holm-Swarm (EHS) tumor
Protein Concentration
6 mg/mL
Endotoxin Level
≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
Tested following USP <71> sterility guidelines.
Testing Cell Culture
3-D Culture - Laminin I Supports differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) into acinar structures.

Cell Attachment - Tested for the ability to Supports cell attachment and spreading of MG63 human osteosarcoma cells.
Stability
Product is stable for a minimum of 3 months from date of shipment. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store at ≤ -20 °C in a manual defrost freezer. For optimal stability, store at ≤ -70 °C. Avoid freeze-thaw cycles
Species
Mouse

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Reagents Provided

Other Reagents Provided

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