Cultrex 3-D Culture Matrix Rat Collagen I

Rat Collagen I specifically qualified to support 3D culture applications
Catalog # Availability Size / Price Qty
3447-020-01
Product Details
Citations (8)
FAQs
Reviews

Cultrex 3-D Culture Matrix Rat Collagen I Summary

3-D Culture Matrix Collagen I is an extracellular matrix hydrogel that directs cells to grow in three dimensions and assemble into organotypic structures in vitro.

Why Use Cultrex 3-D Culture Matrix Rat Collagen I?

Cultrex 3-D Culture Matrix Collagen I is purified from rat tail tendons and is provided at a high concentration that is capable of polymerizing to form a hydrogel. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37̊ C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I for use in 3-D cultures, a special process is employed to provide material at a standard concentration of approximately 5 mg/mL. This material is then incorporated in a 3-D culture to validate efficacy.Cultrex 3-D Culture Matrix Collagen I is a purified stromal ECM protein that has been developed, produced and qualified specifically for use in 3-D culture studies. 3-D Culture Matrix Collagen I may also be used to supplement customized hydrogel or medium formulations for cell culture.

Specifications

Source
Murine Engelbreth-Holm-Swarm (EHS) tumor
Protein Concentration
5 mg/mL
Endotoxin Level
≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Testing Cell Culture
3-D Culture - Laminin I Supports differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) into acinar structures.

Cell Attachment - Tested for the ability to Supports cell attachment and spreading of MG63 human osteosarcoma cells.
Viral Testing
Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses.
Stability
Product is stable for a minimum of 3 months if stored at 2-8 °C. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at 2 - 8 °C.
Species
Rat

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Citations for Cultrex 3-D Culture Matrix Rat Collagen I

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Robust and Scalable Angiogenesis Assay of Perfused 3D Human iPSC-Derived Endothelium for Anti-Angiogenic Drug Screening
    Authors: V van Duinen, W Stam, E Mulder, F Famili, A Reijerkerk, P Vulto, T Hankemeier, AJ van Zonnev
    Int J Mol Sci, 2020;21(13):.  2020
  2. Tubuloids derived from human adult kidney and urine for personalized disease modeling
    Authors: F Schutgens, MB Rookmaaker, T Margaritis, A Rios, C Ammerlaan, J Jansen, L Gijzen, M Vormann, A Vonk, M Viveen, FY Yengej, S Derakhshan, KM de Winter-, B Artegiani, R van Boxtel, E Cuppen, APA Hendrickx, MM van den He, E Heitzer, H Lanz, J Beekman, JL Murk, R Masereeuw, F Holstege, J Drost, MC Verhaar, H Clevers
    Nat. Biotechnol., 2019;37(3):303-313.  2019
  3. Genome-Wide CRISPR-Cas9 Screens Expose Genetic Vulnerabilities and Mechanisms of Temozolomide Sensitivity in Glioblastoma Stem Cells
    Authors: G MacLeod, DA Bozek, N Rajakulend, V Monteiro, M Ahmadi, Z Steinhart, MM Kushida, H Yu, FJ Coutinho, FMG Cavalli, I Restall, X Hao, T Hart, HA Luchman, S Weiss, PB Dirks, S Angers
    Cell Rep, 2019;27(3):971-986.e9.  2019
  4. Transient Receptor Potential Channel Expression Signatures in Tumor-Derived Endothelial Cells: Functional Roles in Prostate Cancer Angiogenesis
    Authors: M Bernardini, A Brossa, G Chinigo, GP Grolez, G Trimaglio, L Allart, A Hulot, G Marot, T Genova, A Joshi, V Mattot, G Fromont, L Munaron, B Bussolati, N Prevarskay, A Fiorio Pla, D Gkika
    Cancers (Basel), 2019;11(7):.  2019
  5. A Role of Agrin in Maintaining the Stability of Vascular Endothelial Growth Factor Receptor-2 during Tumor Angiogenesis
    Authors: K Njah, S Chakrabort, B Qiu, S Arumugam, A Raju, AV Pobbati, M Lakshmanan, V Tergaonkar, G Thibault, X Wang, W Hong
    Cell Rep, 2019;28(4):949-965.e7.  2019
  6. Nrf2 and SQSTM1/p62 jointly contribute to mesenchymal transition and invasion in glioblastoma
    Authors: P Pölönen, A Jawahar De, HM Leinonen, HK Jyrkkänen, S Kuosmanen, M Mononen, A Jain, T Tuomainen, S Pasonen-Se, JM Hartikaine, A Mannermaa, M Nykter, P Tavi, T Johansen, M Heinäniemi, AL Levonen
    Oncogene, 2019;0(0):.  2019
  7. Fibulin-6 regulates pro-fibrotic TGF-beta responses in neonatal mouse ventricular cardiac fibroblasts.
    Authors: Chowdhury A, Hasselbach L, Echtermeyer F, Jyotsana N, Theilmeier G, Herzog C
    Sci Rep, 0;7(0):42725.  0
  8. Organotypic culture of untransformed and tumorigenic primary mammary epithelial cells.
    Authors: Jechlinger M
    Cold Spring Harb Protoc, 0;2015(5):457-61.  0

FAQs

  1. What type of analysis is typically applied for organoid or 3-D cell cultures?

    • Within the organoid, spheroid, or 3-D culture, cells may be assessed for morphology, apical/basal polarity, protein localization, and relative proliferation. In addition, cells may be isolated from the 3-D culture and evaluated for levels of RNA and protein expression, as well as modifications to DNA.

  2. What is the difference between the standard Cultrex Rat Collagen I and the Cultrex 3-D Rat Collagen I?

    • Cultrex 3-D Rat Collagen I undergoes the same basic purification and efficacy tests as the standard collagen. However, it undergoes additional 3-D culture validation; it has been tested extensively for the ability to promote growth and differentiation of cell types, visualized by morphology in three dimensions in vitro.

  3. What are 3-D cultures?

    • 3-D cultures are in vitro cultures where immortalized cell lines, primary cell lines, stem cells, or tissue explants are placed within hydrogel matrices, such as Cultrex Basement Membrane Extract, that mimic in vivo cell environments and allow cells to proliferate in three dimensions.

  4. What is the advantage of 3-D culture over traditional 2-D culture?

    • While 2-D culture has been used for studying many aspects of cell function and behavior, the tissue-culture treated plastic environment is unlike anything found within living organisms. As a result, cells in 2-D culture exhibit altered morphology, function, proliferation, and gene expression when compared to their emanating tissues. By placing these cells in a 3-D environment, they assume biological and biochemical characteristics similar to what is observed in vivo.

  5. What are the variables associated with 3-D culture?

    • The major variables associated with 3-D culture are cell type, cell seeding density, composition of hydrogel, thickness of hydrogel, stiffness of hydrogel, composition of cell culture medium, and time of culture.

  6. What are the different types of 3-D culture?

    • The two principal methods for performing 3-D culture are the top assay and embedded assay. For the top assay, cells are seeded on a thick gel of Cultrex Basement Membrane Extract (BME) or Extracellular Matrix Protein. A thin overlay of cell culture medium is then applied to the cells. For the embedded assay, cells are resuspended within a thick gel of Cultrex BME or ECM and the culture media is applied on top. The top assay is easier to setup, to control seeding densities, and to keep cells within one focal plane for analysis.

  7. Which matrix should I use for 3-D culture?

    • Choice of matrix should correspond to the environment that you wish to recapitulate. Cultrex Basement Membrane Extract (BME) will recapitulate the basal lamina, which underlie most cells of epithelial or endothelial origin. Cultrex Collagen I is the major constituent of connective tissue, and is commonly inhabited by stationary cells, such as fibrocytes and adipose cells, as well as migrating cells, such as mast cells, macrophages, monocytes, lymphocytes, plasma cells, and eosinophils.

  8. How should cells be cultured prior to setting up the 3-D culture?

    • Cells need to be healthy and actively dividing in 2-D culture. Cells should be passaged two or three times after resuspension from cryopreservation, and they should never surpass 80% confluency during each passage. Cells should also be assessed for viability using trypan blue, and they should exhibit less than 5% staining.

  9. Will Cultrex 3-D culture Matrix rat Collagen 1, Catalog # 3447-020-01, still work if it has been frozen? 

    • This product will likely not form a gel after being frozen.

  10. Is Cultrex 3-D Culture Matrix Rat Collagen I (Catalog # 3447-020-01) atelocollagen or telocollagen?

    • Cultrex 3-D Culture Matrix Rat Collagen I (Catalog # 3447-020-01) is telocollagen.

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