Cultrex 3-D Culture Matrix Rat Collagen I

Get 30% off Rat Collagen! Use Promo Code: COLLAGEN3
View Terms and Conditions
Catalog # Availability Size / Price Qty
3447-020-01
Product Details
FAQs
Reviews

Cultrex 3-D Culture Matrix Rat Collagen I Summary

3-D Culture Matrix Collagen I is an extracellular matrix hydrogel that directs cells to grow in three dimensions and assemble into organotypic structures in vitro.

Why Use Cultrex 3-D Culture Matrix Rat Collagen I?

Cultrex 3-D Culture Matrix Collagen I is purified from rat tail tendons and is provided at a high concentration that is capable of polymerizing to form a hydrogel. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37̊ C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I for use in 3-D cultures, a special process is employed to provide material at a standard concentration of approximately 5 mg/mL. This material is then incorporated in a 3-D culture to validate efficacy.Cultrex 3-D Culture Matrix Collagen I is a purified stromal ECM protein that has been developed, produced and qualified specifically for use in 3-D culture studies. 3-D Culture Matrix Collagen I may also be used to supplement customized hydrogel or medium formulations for cell culture.

Specifications

Source
Murine Engelbreth-Holm-Swarm (EHS) tumor
Protein Concentration
5 mg/mL
Endotoxin Level
≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Testing Cell Culture
3-D Culture - Laminin I Supports differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) into acinar structures.

Cell Attachment - Tested for the ability to Supports cell attachment and spreading of MG63 human osteosarcoma cells.
Viral Testing
Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses.
Stability
Product is stable for a minimum of 3 months if stored at 2-8 °C. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at 2 - 8 °C.
Species
Rat

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Reagents Provided

Other Reagents Provided

FAQs

No product specific FAQs exist for this product, however you may

View all FAQs

Reviews for Cultrex 3-D Culture Matrix Rat Collagen I

There are currently no reviews for this product. Be the first to review Cultrex 3-D Culture Matrix Rat Collagen I and earn rewards!

Have you used Cultrex 3-D Culture Matrix Rat Collagen I?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review