Cultrex 3-D Culture Matrix Reduced Growth Factor Basement Membrane Extract, Pathclear

Reduced Growth Factor Basement Membrane Extract for 3-D Cell Culture
Catalog # Availability Size / Price Qty
3445-001-01
3445-005-01
3445-010-01
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Cultrex 3-D Culture Matrix Reduced Growth Factor Basement Membrane Extract, Pathclear Summary

Cultrex 3-D Culture Matrix RGF BME is an extracellular matrix hydrogel that directs cells to grow in three dimensions and assemble into organotypic structures in vitro.

Key Benefits

• Qualified to support 3-D cell culture applications
• Reduced growth factor formulation provides a more defined culture system
• Quality controlled for performance consistency

Why Use Cultrex 3-D Culture Matrix RGF BME ?

Cultrex 3-D Culture Matrix Reduced Factor Basement Membrane Extract (RGF BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Cultrex 3-D Culture Matrix RGF BME is produced and qualified specifically for use in 3-D culture studies. It provides the foundation for cells to grow in three dimensions allowing for the formation of complex in vitro structures. To provide the most standardized basement membrane extract for use in 3-D cultures, a special process is employed to reduce growth factors and manufacture matrix at a standard and consistent concentration. This product is then evaluated in 3-D culture assays to validate efficacy. Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.

Specifications

Source
Murine Engelbreth-Holm-Swarm (EHS) tumor
Protein Concentration
8-12 mg/mL as determined by Lowry assay
Endotoxin Level
≤ 8 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
Tested following USP <71> sterility guidelines.
Testing Cell Culture
3-D Culture - Cultrex 3-D Culture Matrix RGF BME promotes differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) or human prostate (PC-3) into acinar structures.

Tube Formation Assay - Cultrex 3-D Culture Matrix RGF BME Supports formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells.

Gelling Assay - Cultrex 3-D Culture Matrix RGF BME gels in less than 30 minutes at 37 °C, and maintains the gelled form in culture medium for a minimum of 14 days at 37 °C.
Viral Testing
Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.
Stability
Product is stable for two years from date of manufacture. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Species
Mouse

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Reagents Provided

Other Reagents Provided

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