Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay, 96-well

R&D Systems | Catalog # 3500-096-K

R&D Systems
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Key Product Details

Features

Provides a more complete and physiologically predictive cancer invasion model.

Species

Mouse

Product Summary

Why Use Cultrex 3-D Spheroid Basememnt Membrane Extract Cell Invasion Assay, 96-well?
The Cultrex 3-D Culture Spheroid Cell Invasion Assay utilizes a 3-D Culture Qualified 96 Well Spheroid Formation Plate alongside a specialized Spheroid Formation Extracellular Matrix to drive aggregation and/or spheroid formation of cells. Upon completion of spheroid formation, the spheroid is embedded in an invasion matrix composed of basement membrane proteins. This matrix forms a hydrogel network on which invasive cells can travel. Invasion modulating agents can be applied to the system to evaluate the impact on cell response. Cell invasion is visualized microscopically and can be quantified through image analysis software.

Scientific Data Images

Spheroid Cell Invasion Assay_3500-096-K

Embedding Spheroids in Cultrex BME for Cell Invasion and Migration Assays.

MDA-MB-231 (3,000 cells/well) formed spheroids in low adhesion plates for 72 hours using reagents in the Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay. Briefly MDA-MB-231 cells were cultured in the presence of 1X Spheroid Formation Matrix. Then spheroids were embedded in A) no Invasion Matrix, or B) Spheroid Invasion Matrix. After hydrogel polymerization, DMEM and 10% FBS was added to each well to promote a chemotactic response over a 96 hour period. Note the addition of the Spheroid Invasion Matrix enhanced cell invasion over spheroids cultured without embedding in the invasion matrix.

Kit Contents

  • Cultrex Spheroid Formation Matrix
  • 3-D Culture Qualified 96 Well Spheroid Formation Plate with Strip Seals
  • Cultrex Spheroid Invasion Matrix

    Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.

Formulation, Preparation, and Storage

Shipping

The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.

Storage

Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Basement Membrane Extracts

Basement membrane extract (BME) is a type of extracellular matrix (ECM) derived from Engelbreth-Holm-Swarm mouse sarcomas. BME is commonly used in cell culture and tissue engineering to provide a 2-D or 3-D scaffold for cells, allowing them to grow, differentiate, and interact in an environment that closely mimics the native ECM. This makes BME invaluable for modeling the behavior of cells in vivo.  All Cultrex BME formulations are rigorously tested to ensure performance, quality, and lot-to-lot consistency.

Additional Basement Membrane Extracts Products

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Citations for Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay, 96-well

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  • Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay, 96-well
    Name: Anonymous
    Verified Customer | Posted 04/30/2024
    Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay, 96-well 3500-096-K
  • Cultrex 3D Culture BME Cell Invasion Assay, 96 well
    Name: Anonymous
    Verified Customer | Posted 04/02/2018
    easy to use, excellent result
    Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay, 96-well 3500-096-K

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FAQs

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  • Q: For Cultrex 3D BME Cell Invasion Assay, 96 well,catalog # 3500-096-K,  do we measure the protein concentration of the invasion matrix (part # 3500-096-03) supplied in this kit?

    A: No. We donot measure the protein concentration of the invasion matrix (part # 3500-096-03) supplied in catalog # 3500-096-K.

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