Basement membrane extract (BME) is a type of extracellular matrix (ECM) derived from Engelbreth-Holm-Swarm mouse sarcomas. BME is commonly used in cell culture and tissue engineering to provide a 2-D or 3-D scaffold for cells, allowing them to grow, differentiate, and interact in an environment that closely mimics the native ECM. This makes BME invaluable for modeling the behavior of cells in vivo. All Cultrex BME formulations are rigorously tested to ensure performance, quality, and lot-to-lot consistency.
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Cultrex Basement Membrane Extract, PathClear
R&D Systems | Catalog # 3432-010-01
Basement Membrane Extract for use in multiple culture applications
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Key Product Details
Features
Cultrex Basement Membrane Extract (BME) is an extracellular matrix hydrogel that has been used extensively in general cell culture applications to study cell attachment, proliferation, and invasion.
Key Benefits
- Ideal for general cell culture application
- Polymerizes at 37°C to form a reconstituted basement membrane
- Quality controlled for peformance consistency
Species
Mouse
Product Summary for Cultrex Basement Membrane Extract, PathClear
Why Use Cultrex BME?
Cultrex Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Cultrex BME can be used in multiple applications, including maintaining growth or promoting differentiation of primary endothelial, epithelial, smooth muscle, stem cells, and organoid/3-D cell cultures. It can also be utilized in cell attachment, neurite outgrowth, angiogenesis, in vitro cell invasion, and in vivo tumorigenicity assays.
Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.
Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.
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Product Specifications
Source
Murine Engelbreth-Holm-Swarm (EHS) tumor
Protein Concentration
8-12 mg/mL as determined by Lowry assay
Endotoxin Level
≤ 8 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Viral Testing
Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.
Formulation, Preparation, and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Stability
Product is stable for at least two years from date of manufacture when stored at ≤ -70 °C. See lot specific Certificate of Analysis for expiration date.
Background: Basement Membrane Extracts
Additional Basement Membrane Extracts Products
Product Documents for Cultrex Basement Membrane Extract, PathClear
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Citations for Cultrex Basement Membrane Extract, PathClear
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Verified Customer | Posted 05/23/2022Used for 3D culture experiments. Went very well
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Verified Customer | Posted 02/01/2022for 3D cell culture
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Application: In vivo studyVerified Customer | Posted 08/20/2018
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Application: Cell migration/motilityVerified Customer | Posted 04/01/2018
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FAQs for Cultrex Basement Membrane Extract, PathClear
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Q: How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?
A: All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.
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Q: What is the percent of Laminin I in Cultrex® Basement Membrane Extract, PathClear, Catalog # 3432-010-01?
A: Cultrex® Basement Membrane Extract (BME), Pathclear, is composed of ≤ 60 % of Laminin I. Laminin is the major component of Cultrex BME. Other components are Collagen IV, which makes up ≤ 40% and Entactin which makes up ≤ 10%.
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Q: How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?
A: All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.
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Q: What is the percent of Laminin I in Cultrex® Basement Membrane Extract, PathClear, Catalog # 3432-010-01?
A: Cultrex® Basement Membrane Extract (BME), Pathclear, is composed of ≤ 60 % of Laminin I. Laminin is the major component of Cultrex BME. Other components are Collagen IV, which makes up ≤ 40% and Entactin which makes up ≤ 10%.