Cultrex Basement Membrane Extract, PathClear

Basement Membrane Extract for use in multiple culture applications
Catalog # Availability Size / Price Qty
3432-001-01
3432-005-01
3432-010-01

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Cultrex Basement Membrane Extract
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Product Details
Citations (40)
FAQs
Reviews (6)

Cultrex Basement Membrane Extract, PathClear Summary

Cultrex Basement Membrane Extract (BME) is an extracellular matrix hydrogel that has been used extensively in general cell culture applications to study cell attachment, proliferation, and invasion.
 

Key Benefits

• Ideal for general cell culture application
• Polymerizes at 37°C to form a reconstituted basement membrane
• Quality controlled for peformance consistency

Why Use Cultrex BME?

Cultrex Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Cultrex BME can be used in multiple applications, including maintaining growth or promoting differentiation of primary endothelial, epithelial, smooth muscle, stem cells, and organoid/3-D cell cultures. It can also be utilized in cell attachment, neurite outgrowth, angiogenesis, in vitro cell invasion, and in vivo tumorigenicity assays.

Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.

Specifications

Source
Murine Engelbreth-Holm-Swarm (EHS) tumor
Protein Concentration
8-12 mg/mL as determined by Lowry assay
Endotoxin Level
≤ 8 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Testing Cell Culture
Tube Formation Assay - Cultrex BME supports formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells in a tube formation assay.
Viral Testing
Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.
Stability
Product is stable for at least two years from date of manufacture when stored at ≤ -70 °C. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Species
Mouse

Limitations

For research use only. Not for diagnostic use.

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Citations for Cultrex Basement Membrane Extract, PathClear

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

40 Citations: Showing 1 - 10
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  1. Oropouche orthobunyavirus infection is mediated by the cellular host factor Lrp1
    Authors: MM Schwarz, DA Price, SS Ganaie, A Feng, N Mishra, RM Hoehl, F Fatma, SH Stubbs, SPJ Whelan, X Cui, T Egawa, DW Leung, GK Amarasingh, AL Hartman
    Proceedings of the National Academy of Sciences of the United States of America, 2022;119(33):e2204706119.  2022
  2. Development of a Gene-Activated Scaffold Incorporating Multifunctional Cell-Penetrating Peptides for pSDF-1alpha Delivery for Enhanced Angiogenesis in Tissue Engineering Applications
    Authors: RN Power, BL Cavanagh, JE Dixon, CM Curtin, FJ O'Brien
    International Journal of Molecular Sciences, 2022;23(3):.  2022
  3. Maladaptive functional changes in alveolar fibroblasts due to perinatal hyperoxia impair epithelial differentiation
    Authors: MR Riccetti, MG Ushakumary, M Waltamath, J Green, J Snowball, SE Dautel, M Endale, B Lami, J Woods, SK Ahlfeld, AT Perl
    JCI Insight, 2022;0(0):.  2022
  4. Extracellular Matrix Components Regulate Bone Sialoprotein Expression in MDA-MB-231 Breast Cancer Cells
    Authors: F Keller, R Bruch, F Clauder, M Hafner, R Rudolf
    Cells, 2021;10(6):.  2021
  5. Obesity alters ovarian folliculogenesis through disrupted angiogenesis from increased IL-10 production
    Authors: PK Yang, CH Chou, CC Huang, WF Wen, HF Chen, CT Shun, HN Ho, MJ Chen
    Molecular Metabolism, 2021;49(0):101189.  2021
  6. Basement membrane proteins as a substrate for efficient Trypanosoma brucei differentiation in vitro
    Authors: F Rojas, M Cayla, KR Matthews
    PloS Neglected Tropical Diseases, 2021;15(4):e0009284.  2021
  7. Specific and Non-Invasive Fluorescent Labelling of Extracellular Vesicles for Evaluation of Intracellular Processing by Intestinal Epithelial Cells
    Authors: MS Hansen, ISE Gadegaard, EC Arnspang, K Blans, LN Nejsum, JT Rasmussen
    Biomedicines, 2020;8(7):.  2020
  8. New generation breast cancer cell lines developed from patient-derived xenografts
    Authors: J Finlay-Sch, BM Jacobsen, D Riley, KV Paul, S Turner, A Ferreira-G, JC Harrell, P Kabos, CA Sartorius
    Breast Cancer Res., 2020;22(1):68.  2020
  9. Analysis of protein chlorination by mass spectrometry
    Authors: T Nybo, MJ Davies, A Rogowska-W
    Redox Biol, 2019;26(0):101236.  2019
  10. Mitophagy regulates mitochondrial network signaling, oxidative stress, and apoptosis during myoblast differentiation
    Authors: BL Baechler, D Bloemberg, J Quadrilate
    Autophagy, 2019;0(0):.  2019
  11. Transposon mutagenesis identifies genes that cooperate with mutant Pten in breast cancer progression
    Proc. Natl. Acad. Sci. U.S.A., 2016;0(0):.  2016
  12. Wnt signaling promotes breast cancer by blocking ITCH-mediated degradation of the YAP/TAZ transcriptional coactivator WBP2
    Cancer Res, 2016;0(0):.  2016
  13. Cabozantinib (XL184) Inhibits Growth and Invasion of Preclinical TNBC Models.
    Authors: Sameni M, Tovar E, Essenburg C, Chalasani A, Linklater E, Borgman A, Cherba D, Anbalagan A, Winn M, Graveel C, Sloane B
    Clin Cancer Res, 2015;22(4):923-34.  2015
  14. The M33 G protein-coupled receptor encoded by murine cytomegalovirus is dispensable for hematogenous dissemination but is required for growth within the salivary gland.
    Authors: Bittencourt F, Wu S, Bridges J, Miller W
    J Virol, 2014;88(20):11811-24.  2014
  15. Simian immunodeficiency virus and human immunodeficiency virus type 1 matrix proteins specify different capabilities to modulate B cell growth.
    Authors: Caccuri, Francesc, Giagulli, Cinzia, Reichelt, Joachim, Martorelli, Debora, Marsico, Stefania, Bugatti, Antonell, Barone, Ines, Rusnati, Marco, Guzman, Carlos A, Dolcetti, Riccardo, Caruso, Arnaldo
    J Virol, 2014;88(10):5706-17.  2014
  16. Suppression of the epithelial-mesenchymal transition by Grainyhead-like-2.
    Authors: Cieply B, Riley P, Pifer P, Widmeyer J, Addison J, Ivanov A, Denvir J, Frisch S
    Cancer Res, 0;72(9):2440-53.  0
  17. Dickkopf-3 regulates prostate epithelial cell acinar morphogenesis and prostate cancer cell invasion by limiting TGF-beta-dependent activation of matrix metalloproteases.
    Authors: Romero D, Al-Shareef Z, Gorrono-Etxebarria I, Atkins S, Turrell F, Chhetri J, Bengoa-Vergniory N, Zenzmaier C, Berger P, Waxman J, Kypta R
    Carcinogenesis, 0;37(1):18-29.  0
  18. Regulation of P21-activated kinase-4 by progesterone and tumor necrosis factor-alpha in human endometrium and its increased expression in advanced-stage endometriosis.
    Authors: Kim S, Kim S, Ihm H, Oh Y, Chae H, Kim C, Kang B
    J Clin Endocrinol Metab, 0;98(2):E238-48.  0
  19. Anti-invasive adjuvant therapy with imipramine blue enhances chemotherapeutic efficacy against glioma.
    Authors: Munson J, Fried L, Rowson S, Bonner M, Karumbaiah L, Diaz B, Courtneidge S, Knaus U, Brat D, Arbiser J, Bellamkonda R
    Sci Transl Med, 0;4(127):127ra36.  0
  20. p16 controls epithelial cell growth and suppresses carcinogenesis through mechanisms that do not require RB1 function.
    Authors: Sen M, Akeno N, Reece A, Miller A, Simpson D, Wikenheiser-Brokamp K
    Oncogenesis, 0;6(4):e320.  0
  21. A humanized single-chain antibody against beta 3 integrin inhibits pulmonary metastasis by preferentially fragmenting activated platelets in the tumor microenvironment.
    Authors: Zhang W, Dang S, Hong T, Tang J, Fan J, Bu D, Sun Y, Wang Z, Wisniewski T
    Blood, 0;120(14):2889-98.  0
  22. The nuclear receptor peroxisome proliferator-activated receptor-beta/delta (PPARbeta/delta) promotes oncogene-induced cellular senescence through repression of endoplasmic reticulum stress.
    Authors: Zhu B, Ferry C, Markell L, Blazanin N, Glick A, Gonzalez F, Peters J
    J Biol Chem, 0;289(29):20102-19.  0
  23. MELK-T1, a small-molecule inhibitor of protein kinase MELK, decreases DNA-damage tolerance in proliferating cancer cells.
    Authors: Beke L, Kig C, Linders J, Boens S, Boeckx A, van Heerde E, Parade M, De Bondt A, Van den Wyngaert I, Bashir T, Ogata S, Meerpoel L, Van Eynde A, Johnson C, Beullens M, Brehmer D, Bollen M
    Biosci Rep, 0;35(6):.  0
  24. Preclinical Antitumor Efficacy of BAY 1129980-a Novel Auristatin-Based Anti-C4.4A (LYPD3) Antibody-Drug Conjugate for the Treatment of Non-Small Cell Lung Cancer.
    Authors: Willuda J, Linden L, Lerchen H, Kopitz C, Stelte-Ludwig B, Pena C, Lange C, Golfier S, Kneip C, Carrigan P, McLean K, Schuhmacher J, von Ahsen O, Muller J, Dittmer F, Beier R, El Sheikh S, Tebbe J, Leder G, Apeler H, Jautelat R, Ziegelbauer K, Kreft B
    Mol Cancer Ther, 0;16(5):893-904.  0
  25. Inhibition of tumor growth and angiogenesis by SP-2, an anti-lectin, galactoside-binding soluble 3 binding protein (LGALS3BP) antibody.
    Authors: Traini S, Piccolo E, Tinari N, Rossi C, La Sorda R, Spinella F, Bagnato A, Lattanzio R, D'Egidio M, Di Risio A, Tomao F, Grassadonia A, Piantelli M, Natoli C, Iacobelli S
    Mol Cancer Ther, 0;13(4):916-25.  0
  26. Translation of a tumor microenvironment mimicking 3D tumor growth co-culture assay platform to high-content screening.
    Authors: Krausz E, de Hoogt R, Gustin E, Cornelissen F, Grand-Perret T, Janssen L, Vloemans N, Wuyts D, Frans S, Axel A, Peeters P, Hall B, Cik M
    J Biomol Screen, 0;18(1):54-66.  0
  27. Suppression of ERbeta signaling via ERbeta knockout or antagonist protects against bladder cancer development.
    Authors: Hsu I, Chuang K, Slavin S, Da J, Lim W, Pang S, O'Brien J, Yeh S
    Carcinogenesis, 0;35(3):651-61.  0
  28. Microfluidics separation reveals the stem-cell-like deformability of tumor-initiating cells.
    Authors: Zhang W, Kai K, Choi D, Iwamoto T, Nguyen Y, Wong H, Landis M, Ueno N, Chang J, Qin L
    Proc Natl Acad Sci U S A, 0;109(46):18707-12.  0
  29. Dual Targeting of Mesenchymal and Amoeboid Motility Hinders Metastatic Behavior.
    Authors: Jones B, Kelley L, Loskutov Y, Marinak K, Kozyreva V, Smolkin M, Pugacheva E
    Mol Cancer Res, 0;15(6):670-682.  0
  30. Markers of oxidative stress in umbilical cord blood from G6PD deficient African newborns.
    Authors: Stadem P, Hilgers M, Bengo D, Cusick S, Ndidde S, Slusher T, Lund T
    PLoS ONE, 0;12(2):e0172980.  0
  31. Involvement of the heparanase procoagulant domain in bleeding and wound healing.
    Authors: Crispel Y, Ghanem S, Attias J, Kogan I, Brenner B, Nadir Y
    J Thromb Haemost, 0;15(7):1463-1472.  0
  32. Timing and targeting: the biological functions of Dam methylation in E. coli.
    Authors: Messer W, Noyer-Weidner M
    Cell, 0;54(6):735-7.  0
  33. Mouse embryonic dorsal root ganglia contain pluripotent stem cells that show features similar to embryonic stem cells and induced pluripotent stem cells.
    Authors: Ogawa R, Fujita K, Ito K
    Biol Open, 0;6(5):602-618.  0
  34. The interplay between hypoxia, endothelial and melanoma cells regulates vascularization and cell motility through endothelin-1 and vascular endothelial growth factor.
    Authors: Spinella F, Caprara V, Cianfrocca R, Rosano L, Di Castro V, Garrafa E, Natali P, Bagnato A
    Carcinogenesis, 0;35(4):840-8.  0
  35. Cathepsin B inhibition limits bone metastasis in breast cancer.
    Authors: Withana N, Blum G, Sameni M, Slaney C, Anbalagan A, Olive M, Bidwell B, Edgington L, Wang L, Moin K, Sloane B, Anderson R, Bogyo M, Parker B
    Cancer Res, 0;72(5):1199-209.  0
  36. Real-time monitoring of NKCC2 endocytosis by total internal reflection fluorescence (TIRF) microscopy.
    Authors: Jaykumar A, Caceres P, Sablaban I, Tannous B, Ortiz P
    Am J Physiol Renal Physiol, 0;310(2):F183-91.  0
  37. Wnt5a Drives an Invasive Phenotype in Human Glioblastoma Stem-like Cells.
    Authors: Binda E, Visioli A, Giani F, Trivieri N, Palumbo O, Restelli S, Dezi F, Mazza T, Fusilli C, Legnani F, Carella M, Di Meco F, Duggal R, Vescovi A
    Cancer Res, 0;77(4):996-1007.  0
  38. HIV-1 matrix protein p17 promotes angiogenesis via chemokine receptors CXCR1 and CXCR2.
    Authors: Caccuri F, Giagulli C, Bugatti A, Benetti A, Alessandri G, Ribatti D, Marsico S, Apostoli P, Slevin M, Rusnati M, Guzman C, Fiorentini S, Caruso A
    Proc Natl Acad Sci U S A, 0;109(36):14580-5.  0
  39. PI3K/mTOR dual inhibitor VS-5584 preferentially targets cancer stem cells.
    Authors: Kolev V, Wright Q, Vidal C, Ring J, Shapiro I, Ricono J, Weaver D, Padval M, Pachter J, Xu Q
    Cancer Res, 0;75(2):446-55.  0
  40. The MEF2-HDAC axis controls proliferation of mammary epithelial cells and acini formation in vitro.
    Authors: Clocchiatti A, Di Giorgio E, Viviani G, Streuli C, Sgorbissa A, Picco R, Cutano V, Brancolini C
    J Cell Sci, 0;128(21):3961-76.  0

FAQs

  1. What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?

    • Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.

  2. How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?

    • All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.

  3. How should Cultrex Basement Membrane Extract (BME) be stored and handled?

    • Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.

  4. What is the percent of Laminin I in Cultrex® Basement Membrane Extract, PathClear, Catalog # 3432-010-01?

    • Cultrex® Basement Membrane Extract (BME), Pathclear, is composed of ≤ 60 % of Laminin I. Laminin is the major component of Cultrex BME. Other components are Collagen IV, which makes up ≤ 40% and Entactin which makes up ≤ 10%.

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Reviews for Cultrex Basement Membrane Extract, PathClear

Average Rating: 4.8 (Based on 6 Reviews)

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Cultrex Basement Membrane Extract, PathClear
By Anonymous on 11/06/2022

Cultrex Basement Membrane Extract, PathClear
By Anonymous on 05/23/2022

Used for 3D culture experiments. Went very well


Cultrex Basement Membrane Extract, PathClear
By Anonymous on 02/01/2022

for 3D cell culture


Cultrex PathClear Basement Membrane Extract (2 x 5 mL)
By Anonymous on 08/20/2018
Application: In vivo study

Cultrex PathClear Basement Membrane Extract
By Leslie Priddy on 04/01/2018
Application: Cell migration/motility

Cultrex PathClear Basement Membrane Extract
By Leslie Priddy on 04/01/2018
Application: Cell Proliferation