Cultrex Basement Membrane Extract, Type 2, Pathclear, with Phenol Red
Cultrex Basement Membrane Extract, Type 2, Pathclear, with Phenol Red SummaryCultrex Basement Membrane Extract (BME), Type 2 is specifically designed to support the establishment and expansion of robust organoid cultures. It's composition mimics the in vivo microenvironment to improve take rate and growth of organoid progenitor cells.
Why Use Cultrex BME, Type 2?
Cultrex Basement Membrane Extract (BME), Type 2 is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparan sulfate proteoglycans.
Cultrex RGF BME, Type 2 provides a proprietary formulation that is high in tensile strength and is designed for use in robust tissue organoid culture as well as other applications requiring an extracellular matrix scaffold.
• Cultrex Basement Membrane Extract (BME), Type 2
• Phenol Red
Gelling Assay - Cultrex BME, Type 2 gels in less than 30 minutes at 37 °C, and maintains the gelled form in culture medium for a minimum of 7 days at 37 °C.
Dome Assay Cultrex BME, Type 2 forms and maintains stable 3-D dome structures on cell culture plates.
Tube Formation Assay - Cultrex BME, Type 2 supports formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells.
For research use only. Not for diagnostic use.
What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?
Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.
How should Cultrex Basement Membrane Extract (BME) be stored and handled?
Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.
What type of culture plates are recommended for organoid culture and other cultures using Cultrex BME products?
Any plate that is tissue culture treated will bind Cultrex domes/Cultrex coatings. Non-treated or low attachment plates are not recommended for organoid growth and for other cell cultures using coated BME. The domes will float away if non-treated or low-attachment plates are used. Bio-Techne has used the folllowing plates: Corning 96-well, Catalog # 3595; Corning 24-well, Catalog #3526; ThermoFisher 6-well, Catalog # 140675.
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