Cultrex Basement Membrane Extract, Type 2, Pathclear

For Robust Organoid Cultures - Basement Membrane Extract
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Catalog # Availability Size / Price Qty
3532-001-02
3532-005-02
3532-010-02

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Cultrex Basement Membrane Extract, Type 2
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Product Details
Citations (18)
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Reviews (1)

Cultrex Basement Membrane Extract, Type 2, Pathclear Summary

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Stability & Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Citations for Cultrex Basement Membrane Extract, Type 2, Pathclear

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. A Colonic Organoid Model Challenged with the Large Toxins of Clostridioides difficile TcdA and TcdB Exhibit Deregulated Tight Junction Proteins
    Authors: Schneemann, M;Heils, L;Moos, V;Wei beta, F;Krug, SM;Weiner, J;Beule, D;Gerhard, R;Schulzke, JD;Bücker, R;
    Toxins  2023-11-04
  2. A Protocol for Organoids from the Urine of Bladder Cancer Patients
    Authors: Walz, S;Pollehne, P;Geng, R;Schneider, J;Maas, M;Aicher, WK;Stenzl, A;Amend, B;Harland, N;
    Cells  2023-08-31
  3. TRPV1 controls innate immunity during Citrobacter rodentium enteric infection
    Authors: Cremin, M;Tay, E;Ramirez, VT;Murray, K;Nichols, RK;Brust-Mascher, I;Reardon, C;
    bioRxiv : the preprint server for biology  2023-07-27
  4. ATG16L1 protects from interferon-gamma-induced cell death in the small intestinal crypt
    Authors: EG Foerster, DKL Tsang, S Goyal, SJ Robertson, LM Robert, H Maughan, CJ Streutker, SE Girardin, DJ Philpott
    Mucosal Immunology, 2023-02-14;0(0):.  2023-02-14
  5. Modeling bile duct ischemia and reoxygenation injury in�human cholangiocyte organoids for screening of novel cholangio-protective agents
    Authors: S Shi, HP Roest, TPP van den Bo, MJC Bijvelds, MU Boehnert, J de Jonge, SO Dekker, AAF de Vries, HR de Jonge, MMA Verstegen, LJW van der La
    EBioMedicine, 2023-01-04;88(0):104431.  2023-01-04
  6. The molecular network of the proteasome machinery inhibition response is orchestrated by HSP70, revealing vulnerabilities in cancer cells
    Authors: M Oro?, M Grochowski, A Jaiswar, J Legierska, K Jastrz?bsk, M Nowak-Niez, M Ko?os, W Ka?miercza, T Olesi?ski, M Lenarcik, M Cybulska, M Mikula, A ?ylicz, M Mi?czy?ska, K Zettl, JR Wi?niewski, D Walerych
    Cell Reports, 2022-09-27;40(13):111428.  2022-09-27
  7. Jejunum-derived NF-kappaB reporter organoids as 3D models for the study of TNF-alpha-induced inflammation
    Authors: H Daghero, F Doffe, B Varela, V Yozzi, JM Verdes, M Crispo, M Bollati-Fo, R Pagotto
    Scientific Reports, 2022-08-24;12(1):14425.  2022-08-24
  8. The cGAS-STING pathway drives type I IFN immunopathology in COVID-19
    Authors: J Di Domizio, MF Gulen, F Saidoune, VV Thacker, A Yatim, K Sharma, T Nass, E Guenova, M Schaller, C Conrad, C Goepfert, L De Leval, C von Garnie, S Berezowska, A Dubois, M Gilliet, A Ablasser
    Nature, 2022-01-19;0(0):.  2022-01-19
  9. Clinical stage drugs targeting inhibitor of apoptosis proteins purge episomal Hepatitis B viral genome in preclinical models
    Authors: MP Clark, T Huynh, S Rao, L Mackiewicz, H Mason, S Romal, MD Stutz, SH Ahn, L Earnest, V Sozzi, M Littlejohn, BM Tran, N Wiedemann, E Vincan, J Torresi, HJ Netter, T Mahmoudi, P Revill, M Pellegrini, G Ebert
    Cell Death & Disease, 2021-06-23;12(7):641.  2021-06-23
  10. Primary Chicken and Duck Endothelial Cells Display a Differential Response to Infection with Highly Pathogenic Avian Influenza Virus
    Authors: ZWM Tong, AC Karawita, C Kern, H Zhou, JE Sinclair, L Yan, KY Chew, S Lowther, L Trinidad, A Challagull, KA Schat, ML Baker, KR Short
    Genes, 2021-06-10;12(6):.  2021-06-10
  11. A genome-wide CRISPR screen identifies UFMylation and TRAMP-like complexes as host factors required for hepatitis A virus infection
    Authors: J Kulsuptrak, R Wang, NL Meyers, M Ott, AS Puschnik
    Cell Reports, 2021-03-16;34(11):108859.  2021-03-16
  12. Functional Radiogenetic Profiling Implicates ERCC6L2 in Non-homologous End Joining
    Authors: P Francica, M Mutlu, VA Blomen, C Oliveira, Z Nowicka, A Trenner, NM Gerhards, P Bouwman, E Stickel, ML Hekkelman, L Lingg, I Klebic, M van de Ven, R de Korte-G, D Howald, J Jonkers, AA Sartori, W Fendler, JR Chapman, T Brummelkam, S Rottenberg
    Cell Rep, 2020-08-25;32(8):108068.  2020-08-25
  13. High glucose levels increase influenza-associated damage to the pulmonary epithelial-endothelial barrier
    Authors: KD Hulme, L Yan, RJ Marshall, CJ Bloxham, KR Upton, SZ Hasnain, H Bielefeldt, Z Loh, K Ronacher, KY Chew, LA Gallo, KR Short
    Elife, 2020-07-22;9(0):.  2020-07-22
  14. LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer
    Authors: X Jin, LP Ge, DQ Li, ZM Shao, GH Di, XE Xu, YZ Jiang
    Mol. Cancer, 2020-05-11;19(1):87.  2020-05-11
  15. Breast cancer organoids from a patient with giant papillary carcinoma as a high-fidelity model
    Authors: X Li, B Pan, X Song, N Li, D Zhao, M Li, Z Zhao
    Cancer Cell Int., 2020-03-18;20(0):86.  2020-03-18
  16. Radiosensitivity Is an Acquired Vulnerability of PARPi-Resistant BRCA1-Deficient Tumors
    Authors: M Barazas, A Gasparini, Y Huang, A Küçükosman, S Annunziato, P Bouwman, W Sol, A Kersbergen, N Proost, R de Korte-G, M van de Ven, J Jonkers, GR Borst, S Rottenberg
    Cancer Res., 2018-12-10;79(3):452-460.  2018-12-10
  17. Basement Membrane-Based Glucose Sensor Coatings Enhance Continuous Glucose Monitoring in Vivo.
    Authors: Klueh U, Qiao Y, Czajkowski C, Ludzinska I, Antar O, Kreutzer D
    J Diabetes Sci Technol, 2015-08-25;9(5):957-65.  2015-08-25
  18. In vitro differentiation of human amniotic epithelial cells into insulin-producing 3D spheroids.
    Authors: Okere B, Alviano F, Costa R, Quaglino D, Ricci F, Dominici M, Paolucci P, Bonsi L, Iughetti L
    Int J Immunopathol Pharmacol, 2015-07-27;28(3):390-402.  2015-07-27

FAQs

  1. What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?

    • Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.

  2. How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?

    • All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.

  3. How should Cultrex Basement Membrane Extract (BME) be stored and handled?

    • Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.

  4. What type of culture plates are recommended for organoid culture and other cultures using Cultrex BME products?

    • Any plate that is tissue culture treated will bind Cultrex domes/Cultrex coatings. Non-treated or low attachment plates are not recommended for organoid growth and for other cell cultures using coated BME.  The domes will float away if non-treated or low-attachment plates are used. Bio-Techne has used the folllowing plates: Corning 96-well, Catalog # 3595;  Corning 24-well, Catalog #3526;  ThermoFisher 6-well,  Catalog # 140675.

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Reviews for Cultrex Basement Membrane Extract, Type 2, Pathclear

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Cultrex Basement Membrane Extract, Type 2
By Anonymous on 08/15/2023

We tested Cultrex when we have a back order of Matrigel and the organoids grow similarly as they are in Matrigel.