Cultrex Basement Membrane Extract, Type 2, Pathclear
Cultrex Basement Membrane Extract, Type 2, Pathclear Summary
• Qualified for use in organoid cell culture
• Commonly used robust and established organoid systems
• Quality controlled for peformance consistency
Why Use Cultrex BME, Type 2?
Cultrex Basement Membrane Extract (BME), Type 2 is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparan sulfate proteoglycans.
Cultrex RGF BME, Type 2 provides a proprietary formulation that is high in tensile strength and is designed for use in robust tissue organoid culture as well as other applications requiring an extracellular matrix scaffold.
Gelling Assay - Cultrex BME, Type 2 gels in less than 30 minutes at 37 °C, and maintains the gelled form in culture medium for a minimum of 14 days at 37 °C.
Dome Assay Cultrex BME, Type 2 forms and maintains stable 3-D dome structures on cell culture plates.
Tube Formation Assay - Cultrex BME, Type 2 supports formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells.
For research use only. Not for diagnostic use.
Citations for Cultrex Basement Membrane Extract, Type 2, Pathclear
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 10
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Primary Chicken and Duck Endothelial Cells Display a Differential Response to Infection with Highly Pathogenic Avian Influenza Virus
Authors: ZWM Tong, AC Karawita, C Kern, H Zhou, JE Sinclair, L Yan, KY Chew, S Lowther, L Trinidad, A Challagull, KA Schat, ML Baker, KR Short
Genes, 2021;12(6):. 2021
Clinical stage drugs targeting inhibitor of apoptosis proteins purge episomal Hepatitis B viral genome in preclinical models
Authors: MP Clark, T Huynh, S Rao, L Mackiewicz, H Mason, S Romal, MD Stutz, SH Ahn, L Earnest, V Sozzi, M Littlejohn, BM Tran, N Wiedemann, E Vincan, J Torresi, HJ Netter, T Mahmoudi, P Revill, M Pellegrini, G Ebert
Cell Death & Disease, 2021;12(7):641. 2021
A genome-wide CRISPR screen identifies UFMylation and TRAMP-like complexes as host factors required for hepatitis A virus infection
Authors: J Kulsuptrak, R Wang, NL Meyers, M Ott, AS Puschnik
Cell Reports, 2021;34(11):108859. 2021
LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer
Authors: X Jin, LP Ge, DQ Li, ZM Shao, GH Di, XE Xu, YZ Jiang
Mol. Cancer, 2020;19(1):87. 2020
Breast cancer organoids from a patient with giant papillary carcinoma as a high-fidelity model
Authors: X Li, B Pan, X Song, N Li, D Zhao, M Li, Z Zhao
Cancer Cell Int., 2020;20(0):86. 2020
High glucose levels increase influenza-associated damage to the pulmonary epithelial-endothelial barrier
Authors: KD Hulme, L Yan, RJ Marshall, CJ Bloxham, KR Upton, SZ Hasnain, H Bielefeldt, Z Loh, K Ronacher, KY Chew, LA Gallo, KR Short
Elife, 2020;9(0):. 2020
Establishment and characterization of breast cancer organoids from a patient with mammary Paget's disease
Authors: B Pan, D Zhao, Y Liu, N Li, C Song, N Li, X Li, M Li, Z Zhao
Cancer Cell Int., 2020;20(0):365. 2020
Functional Radiogenetic Profiling Implicates ERCC6L2 in Non-homologous End Joining
Authors: P Francica, M Mutlu, VA Blomen, C Oliveira, Z Nowicka, A Trenner, NM Gerhards, P Bouwman, E Stickel, ML Hekkelman, L Lingg, I Klebic, M van de Ven, R de Korte-G, D Howald, J Jonkers, AA Sartori, W Fendler, JR Chapman, T Brummelkam, S Rottenberg
Cell Rep, 2020;32(8):108068. 2020
Radiosensitivity Is an Acquired Vulnerability of PARPi-Resistant BRCA1-Deficient Tumors
Authors: M Barazas, A Gasparini, Y Huang, A Küçükosman, S Annunziato, P Bouwman, W Sol, A Kersbergen, N Proost, R de Korte-G, M van de Ven, J Jonkers, GR Borst, S Rottenberg
Cancer Res., 2019;79(3):452-460. 2019
Basement Membrane-Based Glucose Sensor Coatings Enhance Continuous Glucose Monitoring in Vivo.
Authors: Klueh U, Qiao Y, Czajkowski C, Ludzinska I, Antar O, Kreutzer D
J Diabetes Sci Technol, 0;9(5):957-65. 0
In vitro differentiation of human amniotic epithelial cells into insulin-producing 3D spheroids.
Authors: Okere B, Alviano F, Costa R, Quaglino D, Ricci F, Dominici M, Paolucci P, Bonsi L, Iughetti L
Int J Immunopathol Pharmacol, 0;28(3):390-402. 0
What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?
Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.
How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?
All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.
How should Cultrex Basement Membrane Extract (BME) be stored and handled?
Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.
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