Cultrex Basement Membrane Extract, Type 2, Pathclear

For Robust Organoid Cultures - Basement Membrane Extract
Catalog # Availability Size / Price Qty

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Cultrex Basement Membrane Extract, Type 2
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Product Details
Citations (14)

Cultrex Basement Membrane Extract, Type 2, Pathclear Summary

Cultrex Basement Membrane Extract (BME), Type 2 is specifically designed to support the establishment and expansion of robust organoid cultures. It's composition mimics the in vivo microenvironment to improve take rate and growth of organoid progenitor cells.

Key Benefits

• Qualified for use in organoid cell culture
• Commonly used robust and established organoid systems
• Quality controlled for peformance consistency

Why Use Cultrex BME, Type 2?

Cultrex Basement Membrane Extract (BME), Type 2 is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparan sulfate proteoglycans.

Cultrex RGF BME, Type 2 provides a proprietary formulation that is high in tensile strength and is designed for use in robust tissue organoid culture as well as other applications requiring an extracellular matrix scaffold.


Murine Engelbreth-Holm-Swarm (EHS) tumor
Protein Concentration
8-12 mg/mL as determined by Lowry assay
Endotoxin Level
≤ 8 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Testing Cell Culture
Organoid Culture - Cultrex BME, Type 2 supports growth and expansion of mouse small intestine organoid progenitor cells.

Gelling Assay - Cultrex BME, Type 2 gels in less than 30 minutes at 37 °C, and maintains the gelled form in culture medium for a minimum of 7 days at 37 °C.

Dome Assay Cultrex BME, Type 2 forms and maintains stable 3-D dome structures on cell culture plates.

Tube Formation Assay - Cultrex BME, Type 2 supports formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells.
Viral Testing
Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.
Product is stable for at least two years from date of manufacture when stored at ≤ -70 °C. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.


For research use only. Not for diagnostic use.

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Citations for Cultrex Basement Membrane Extract, Type 2, Pathclear

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. The cGAS-STING pathway drives type I IFN immunopathology in COVID-19
    Authors: J Di Domizio, MF Gulen, F Saidoune, VV Thacker, A Yatim, K Sharma, T Nass, E Guenova, M Schaller, C Conrad, C Goepfert, L De Leval, C von Garnie, S Berezowska, A Dubois, M Gilliet, A Ablasser
    Nature, 2022;0(0):.  2022
  2. Jejunum-derived NF-kappaB reporter organoids as 3D models for the study of TNF-alpha-induced inflammation
    Authors: H Daghero, F Doffe, B Varela, V Yozzi, JM Verdes, M Crispo, M Bollati-Fo, R Pagotto
    Scientific Reports, 2022;12(1):14425.  2022
  3. Primary Chicken and Duck Endothelial Cells Display a Differential Response to Infection with Highly Pathogenic Avian Influenza Virus
    Authors: ZWM Tong, AC Karawita, C Kern, H Zhou, JE Sinclair, L Yan, KY Chew, S Lowther, L Trinidad, A Challagull, KA Schat, ML Baker, KR Short
    Genes, 2021;12(6):.  2021
  4. Clinical stage drugs targeting inhibitor of apoptosis proteins purge episomal Hepatitis B viral genome in preclinical models
    Authors: MP Clark, T Huynh, S Rao, L Mackiewicz, H Mason, S Romal, MD Stutz, SH Ahn, L Earnest, V Sozzi, M Littlejohn, BM Tran, N Wiedemann, E Vincan, J Torresi, HJ Netter, T Mahmoudi, P Revill, M Pellegrini, G Ebert
    Cell Death & Disease, 2021;12(7):641.  2021
  5. A genome-wide CRISPR screen identifies UFMylation and TRAMP-like complexes as host factors required for hepatitis A virus infection
    Authors: J Kulsuptrak, R Wang, NL Meyers, M Ott, AS Puschnik
    Cell Reports, 2021;34(11):108859.  2021
  6. LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer
    Authors: X Jin, LP Ge, DQ Li, ZM Shao, GH Di, XE Xu, YZ Jiang
    Mol. Cancer, 2020;19(1):87.  2020
  7. Breast cancer organoids from a patient with giant papillary carcinoma as a high-fidelity model
    Authors: X Li, B Pan, X Song, N Li, D Zhao, M Li, Z Zhao
    Cancer Cell Int., 2020;20(0):86.  2020
  8. High glucose levels increase influenza-associated damage to the pulmonary epithelial-endothelial barrier
    Authors: KD Hulme, L Yan, RJ Marshall, CJ Bloxham, KR Upton, SZ Hasnain, H Bielefeldt, Z Loh, K Ronacher, KY Chew, LA Gallo, KR Short
    Elife, 2020;9(0):.  2020
  9. Establishment and characterization of breast cancer organoids from a patient with mammary Paget's disease
    Authors: B Pan, D Zhao, Y Liu, N Li, C Song, N Li, X Li, M Li, Z Zhao
    Cancer Cell Int., 2020;20(0):365.  2020
  10. Functional Radiogenetic Profiling Implicates ERCC6L2 in Non-homologous End Joining
    Authors: P Francica, M Mutlu, VA Blomen, C Oliveira, Z Nowicka, A Trenner, NM Gerhards, P Bouwman, E Stickel, ML Hekkelman, L Lingg, I Klebic, M van de Ven, R de Korte-G, D Howald, J Jonkers, AA Sartori, W Fendler, JR Chapman, T Brummelkam, S Rottenberg
    Cell Rep, 2020;32(8):108068.  2020
  11. High glucose levels increase influenza-associated damage to the pulmonary epithelial-endothelial barrier
    Authors: KD Hulme, L Yan, RJ Marshall, CJ Bloxham, KR Upton, SZ Hasnain, H Bielefeldt, Z Loh, K Ronacher, KY Chew, LA Gallo, KR Short
    Elife, 2020;9(0):.  2020
  12. Radiosensitivity Is an Acquired Vulnerability of PARPi-Resistant BRCA1-Deficient Tumors
    Authors: M Barazas, A Gasparini, Y Huang, A Küçükosman, S Annunziato, P Bouwman, W Sol, A Kersbergen, N Proost, R de Korte-G, M van de Ven, J Jonkers, GR Borst, S Rottenberg
    Cancer Res., 2019;79(3):452-460.  2019
  13. Basement Membrane-Based Glucose Sensor Coatings Enhance Continuous Glucose Monitoring in Vivo.
    Authors: Klueh U, Qiao Y, Czajkowski C, Ludzinska I, Antar O, Kreutzer D
    J Diabetes Sci Technol, 0;9(5):957-65.  0
  14. In vitro differentiation of human amniotic epithelial cells into insulin-producing 3D spheroids.
    Authors: Okere B, Alviano F, Costa R, Quaglino D, Ricci F, Dominici M, Paolucci P, Bonsi L, Iughetti L
    Int J Immunopathol Pharmacol, 0;28(3):390-402.  0


  1. What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?

    • Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.

  2. How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?

    • All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.

  3. How should Cultrex Basement Membrane Extract (BME) be stored and handled?

    • Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.

  4. What type of culture plates are recommended for organoid culture and other cultures using Cultrex BME products?

    • Any plate that is tissue culture treated will bind Cultrex domes/Cultrex coatings. Non-treated or low attachment plates are not recommended for organoid growth and for other cell cultures using coated BME.  The domes will float away if non-treated or low-attachment plates are used. Bio-Techne has used the folllowing plates: Corning 96-well, Catalog # 3595;  Corning 24-well, Catalog #3526;  ThermoFisher 6-well,  Catalog # 140675.

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