Cultrex Basement Membrane Extract, Type 3, Pathclear

Basement Membrane Extract for xenograft and in vivo tumor modeling
Catalog # Availability Size / Price Qty
3632-001-02
3632-005-02
3632-010-02
Cultrex Basement Membrane Extract, Type 3
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Product Details
Citations (2)
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Reviews (1)

Cultrex Basement Membrane Extract, Type 3, Pathclear Summary

Cultrex Basement Membrane Extract (BME), Type 3 is an extracellular matrix hydrogel that is qualified specifically for use in in vivo xenograft and tumorgraft models.

Key Benefits

• Ideal for xenograft and tumorgraft models
• Designed to mimic the in vivo matrix microenvironment
• Quality controlled for performance consistency

Why Use Cultrex BME, Type 3?

Cultrex Basement Membrane Extract (BME), Type 3 is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Cultrex BME, Type 3 provides a proprietary formulation that is physiologically aligned with the in vivo solid tumor environment and is recommended for xenografts and other in vivo applications. This extracellular matrix hydrogel is designed to help cells to adapt to in vivo transplantation. It mimics the in vivo microenvironment, including low glucose and low pH, to improve take rate and growth of implanted cells for xenograft and tumorgraft models.

Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.

Specifications

Source
Murine Engelbreth-Holm-Swarm (EHS) tumor
Protein Concentration
8-12 mg/mL as determined by Lowry assay
Endotoxin Level
≤ 8 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
Tested following USP <71> sterility guidelines.
Testing Cell Culture
Tumor Growth Assay - Cultrex BME, Type 3 supports proliferation and growth of breast cancer cells (MCF7) embedded in the matrix for minimum of 8 days.

Gelling Assay - Cultrex BME, Type 3 gels in less than 30 minutes at 37 °C, and maintains the gelled form in culture medium for a minimum of 14 days at 37 °C.
Viral Testing
Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.
Stability
Product is stable for a minimum of 3 months after date of receipt when stored at ≤ -70 °C. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Species
Mouse

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Citations for Cultrex Basement Membrane Extract, Type 3, Pathclear

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. GRK2 suppresses lymphomagenesis by inhibiting the MALT1 proto-oncoprotein
    Authors: J Cheng, LR Klei, NE Hubel, M Zhang, R Schairer, LM Maurer, HB Klei, H Kang, VJ Concel, PC Delekta, EV Dang, MA Mintz, M Baens, JG Cyster, N Parameswar, M Thome, PC Lucas, LM McAllister
    J. Clin. Invest., 2020;130(2):1036-1051.  2020
  2. Genetic Identification of SEMA3F as an Antilymphangiogenic Metastasis Suppressor Gene in Head and Neck Squamous Carcinoma.
    Authors: Doci C, Mikelis C, Lionakis M, Molinolo A, Gutkind J
    Cancer Res, 0;75(14):2937-48.  0

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Cultrex Basement Membrane Extract, Type 3, Pathclear
By Anonymous on 07/02/2020
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