Cultrex Cell Migration Assay

Standardized assay for the quantification of cell migration

Discontinued Product

3465-096-K has been discontinued.
View all Basement Membrane Extracts products.
Product Details
Citations (14)
Reviews (2)

Cultrex Cell Migration Assay Summary

24-well and 96-well kits that provides a flexible and standardized format for the quantification of cell migration in response to chemoattractants.

Why Use Cultrex Cell Migration Assay?

Cultrex Cell Migration Assays were originally created in an effort to accelerate the screening process for compounds that influence chemotaxis, which is a fundamental component of angiogenesis in embryonic development, immune responses, and wound healing. These assays offer a flexible, standardized, multi-well format for the quantification of the degree to which cells migrate in vitro in response to chemoattractants in the presence of inhibitors or stimulants. The Cultrex Cell Migration Assays are provided as either a single 96 well plate providing capacity for large screening experiments or as 24 individual inserts providing flexibility for smaller studies.

The Cultrex Cell Migration Assays utilizes a simplified Boyden chamber design with an 8 micron polyethylene terephthalate (PET) membrane. Cells attach to and migrate through the membrane in response to chemokines and growth factors. The porosity of this membrane is most appropriate for adherent epithelial or endothelial cells based on cell size. These assays are available in 24 and 96 well formats.

Kit Contents

• Cell Migration Chamber
• 25X Cell Wash Buffer
• 10X Cell Dissociation Solution
• Calcein AM

Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.


Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.


For research use only. Not for diagnostic use.

Product Datasheets

Citations for Cultrex Cell Migration Assay

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Vadadustat, a HIF Prolyl Hydroxylase Inhibitor, Improves Immunomodulatory Properties of Human Mesenchymal Stromal Cells
    Authors: K Zielniok, A Burdzinska, B Kaleta, R Zagozdzon, L Paczek
    Cells, 2020;9(11):.  2020
  2. EP4 and Class III beta-Tubulin Expression in Uterine Smooth Muscle Tumors: Implications for Prognosis and Treatment
    Authors: J Reader, AK Harper, T Legesse, PN Staats, O Goloubeva, GG Rao, A Fulton, DM Roque
    Cancers (Basel), 2019;11(10):.  2019
  3. Cathepsin L-induced galectin-1 may act as a proangiogenic factor in the metastasis of high-grade serous carcinoma
    Authors: MZI Pranjol, DA Zinovkin, ART Maskell, LJ Stephens, SL Achinovich, DM Los', EA Nadyrov, M Hannemann, NJ Gutowski, JL Whatmore
    J Transl Med, 2019;17(1):216.  2019
  4. MicroRNA-182 targets protein phosphatase 1 regulatory inhibitor subunit 1C in glioblastoma
    Authors: L Liu, X Zhang, C Nan, Z Zhao, S Ma, W Li, H Hu, Z Liang
    Oncotarget, 2017;8(70):114677-114684.  2017
  5. Preclinical evaluation of the imipridone family, analogues of clinical stage anti-cancer small molecule ONC201, reveals potent anti-cancer effects of ONC212
    Authors: J Wagner, CL Kline, MD Ralff, A Lev, A Lulla, L Zhou, GL Olson, BR Nallaganch, CH Benes, JE Allen, VV Prabhu, M Stogniew, W Oster, WS El-Deiry
    Cell Cycle, 2017;0(0):0.  2017
  6. Musashi-2 (MSI2) supports TGF-beta signaling and inhibits claudins to promote non-small cell lung cancer (NSCLC) metastasis.
    Authors: Kudinov A, Deneka A, Nikonova A, Beck T, Ahn Y, Liu X, Martinez C, Schultz F, Reynolds S, Yang D, Cai K, Yaghmour K, Baker K, Egleston B, Nicolas E, Chikwem A, Andrianov G, Singh S, Borghaei H, Serebriiskii I, Gibbons D, Kurie J, Golemis E, Boumber Y
    Proc Natl Acad Sci U S A, 2016;113(25):6955-60.  2016
  7. SIRT6 deacetylates PKM2 to suppress its nuclear localization and oncogenic functions.
    Authors: Bhardwaj A, Das S
    Proc Natl Acad Sci U S A, 0;113(5):E538-47.  0
  8. Biological Activities of Uric Acid in Infection Due to Enteropathogenic and Shiga-Toxigenic Escherichia coli.
    Authors: Crane J, Broome J, Lis A
    Infect Immun, 0;84(4):976-988.  0
  9. Hypermethylation of the GABRE~miR-452~miR-224 promoter in prostate cancer predicts biochemical recurrence after radical prostatectomy.
    Authors: Kristensen H, Haldrup C, Strand S, Mundbjerg K, Mortensen M, Thorsen K, Ostenfeld M, Wild P, Arsov C, Goering W, Visakorpi T, Egevad L, Lindberg J, Gronberg H, Hoyer S, Borre M, Orntoft T, Sorensen K
    Clin Cancer Res, 0;20(8):2169-81.  0
  10. Reprogramming of monocytes by GM-CSF contributes to regulatory immune functions during intestinal inflammation.
    Authors: Dabritz J, Weinhage T, Varga G, Wirth T, Walscheid K, Brockhausen A, Schwarzmaier D, Bruckner M, Ross M, Bettenworth D, Roth J, Ehrchen J, Foell D
    J Immunol, 0;194(5):2424-38.  0
  11. A Novel Peroxisome Proliferator-activated Receptor (PPAR)gamma Agonist 2-Hydroxyethyl 5-chloro-4,5-didehydrojasmonate Exerts Anti-Inflammatory Effects in Colitis.
    Authors: Choo J, Lee Y, Yan X, Noh T, Kim S, Son S, Pothoulakis C, Moon H, Jung J, Im E
    J Biol Chem, 0;290(42):25609-19.  0
  12. MicroRNA-433 inhibits liver cancer cell migration by repressing the protein expression and function of cAMP response element-binding protein.
    Authors: Yang Z, Tsuchiya H, Zhang Y, Hartnett M, Wang L
    J Biol Chem, 0;288(40):28893-9.  0
  13. Fenretinide Perturbs Focal Adhesion Kinase in Premalignant and Malignant Human Oral Keratinocytes. Fenretinide's Chemopreventive Mechanisms Include ECM Interactions.
    Authors: Han B, Li S, Tong M, Holpuch A, Spinney R, Wang D, Border M, Liu Z, Sarode S, Pei P, Schwendeman S, Mallery S
    Cancer Prev Res (Phila), 0;8(5):419-30.  0
  14. MicroRNA miR-183 functions as an oncogene by targeting the transcription factor EGR1 and promoting tumor cell migration.
    Authors: Sarver A, Li L, Subramanian S
    Cancer Res, 0;70(23):9570-80.  0


  1. What is cell migration?

    • Cell migration is the movement of cells in response to a chemical stimulus; also known as chemotaxis. Cultrex® Cell Migration Assays evaluate cell migration based on the cells ability to traverse an uncoated membrane with 8 µm pores, in response to a chemotactic gradient. The cells must undergo cytoskeletal remodeling to fit into the pores and pull themselves through to the underside of the membrane.

  2. Can the migration chamber be coated with fibronectin?

    • Yes, the migration chamber can be coated with fibronectin.

  3. The kit comes with both black and clear microplates.  Do I need to use both?

    • The entire assay may be conducted in either the clear or black assay plate (provided in3455-096-01), if desired.  The black assay plate provides increased sensitivity and reduced background for plate readers that read from the top.  The clear assay plate provides compatibility for plate readers that read from the bottom.  Some researchers have used the clear assay plate for the invasion step and the black assay plate for detection (top read).

  4. Is the migration chamber treated?

    • The Boyden Chamber Insert with 8.0 µm polyethylene terephthalate (PET) membrane is tissue-cuture treated.   For cell invasion assays, this chamber can be treated with extracellular matrix components  Laminin I, Collagen I, Collagen IV, and Basement Membrane Extract (BME), Regular and PathClear®.  Complete cell invasion assays are available for each of these ECM components.

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Reviews for Cultrex Cell Migration Assay

Average Rating: 4 (Based on 2 Reviews)

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Cultrex Cell Migration Assay
By Anonymous on 02/17/2021

Easy to use. The result was publishable.

Cultrex Cell Migration Assay, 96 well
By Anonymous on 05/09/2016