Basement membrane extract (BME) is a type of extracellular matrix (ECM) derived from Engelbreth-Holm-Swarm mouse sarcomas. BME is commonly used in cell culture and tissue engineering to provide a 2-D or 3-D scaffold for cells, allowing them to grow, differentiate, and interact in an environment that closely mimics the native ECM. This makes BME invaluable for modeling the behavior of cells in vivo. All Cultrex BME formulations are rigorously tested to ensure performance, quality, and lot-to-lot consistency.
Cultrex Cell Migration Assay
R&D Systems | Catalog # 3465-096-K
Standardized assay for the quantification of cell migration
Discontinued Product
3465-096-K has been discontinued.
View all Basement Membrane Extracts products.
Key Product Details
Features
24-well and 96-well kits that provides a flexible and standardized format for the quantification of cell migration in response to chemoattractants.
Species
Multi-Species
Product Summary for Cultrex Cell Migration Assay
Why Use Cultrex Cell Migration Assay?
Cultrex Cell Migration Assays were originally created in an effort to accelerate the screening process for compounds that influence chemotaxis, which is a fundamental component of angiogenesis in embryonic development, immune responses, and wound healing. These assays offer a flexible, standardized, multi-well format for the quantification of the degree to which cells migrate in vitro in response to chemoattractants in the presence of inhibitors or stimulants. The Cultrex Cell Migration Assays are provided as either a single 96 well plate providing capacity for large screening experiments or as 24 individual inserts providing flexibility for smaller studies.
The Cultrex Cell Migration Assays utilizes a simplified Boyden chamber design with an 8 micron polyethylene terephthalate (PET) membrane. Cells attach to and migrate through the membrane in response to chemokines and growth factors. The porosity of this membrane is most appropriate for adherent epithelial or endothelial cells based on cell size. These assays are available in 24 and 96 well formats.
The Cultrex Cell Migration Assays utilizes a simplified Boyden chamber design with an 8 micron polyethylene terephthalate (PET) membrane. Cells attach to and migrate through the membrane in response to chemokines and growth factors. The porosity of this membrane is most appropriate for adherent epithelial or endothelial cells based on cell size. These assays are available in 24 and 96 well formats.
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Kit Contents for Cultrex Cell Migration Assay
- Cell Migration Chamber
- 25X Cell Wash Buffer
- 10X Cell Dissociation Solution
- Calcein AM
Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.
Formulation, Preparation, and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Basement Membrane Extracts
Additional Basement Membrane Extracts Products
Product Documents for Cultrex Cell Migration Assay
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Citations for Cultrex Cell Migration Assay
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Verified Customer | Posted 02/17/2021Easy to use. The result was publishable.
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FAQs for Cultrex Cell Migration Assay
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Q: Can the migration chamber be coated with fibronectin?
A: Yes, the migration chamber can be coated with fibronectin.
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Q: Is the migration chamber treated?
A: The Boyden Chamber Insert with 8.0 µm polyethylene terephthalate (PET) membrane is tissue-cuture treated. For cell invasion assays, this chamber can be treated with extracellular matrix components Laminin I, Collagen I, Collagen IV, and Basement Membrane Extract (BME), Regular and PathClear®. Complete cell invasion assays are available for each of these ECM components.
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Q: The kit comes with both black and clear microplates. Do I need to use both?
A: The entire assay may be conducted in either the clear or black assay plate (provided in3455-096-01), if desired. The black assay plate provides increased sensitivity and reduced background for plate readers that read from the top. The clear assay plate provides compatibility for plate readers that read from the bottom. Some researchers have used the clear assay plate for the invasion step and the black assay plate for detection (top read).
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Q: Can the migration chamber be coated with fibronectin?
A: Yes, the migration chamber can be coated with fibronectin.
-
Q: Is the migration chamber treated?
A: The Boyden Chamber Insert with 8.0 µm polyethylene terephthalate (PET) membrane is tissue-cuture treated. For cell invasion assays, this chamber can be treated with extracellular matrix components Laminin I, Collagen I, Collagen IV, and Basement Membrane Extract (BME), Regular and PathClear®. Complete cell invasion assays are available for each of these ECM components.
-
Q: The kit comes with both black and clear microplates. Do I need to use both?
A: The entire assay may be conducted in either the clear or black assay plate (provided in3455-096-01), if desired. The black assay plate provides increased sensitivity and reduced background for plate readers that read from the top. The clear assay plate provides compatibility for plate readers that read from the bottom. Some researchers have used the clear assay plate for the invasion step and the black assay plate for detection (top read).
-
Q: Can the migration chamber be coated with fibronectin?
A: Yes, the migration chamber can be coated with fibronectin.
-
Q: Is the migration chamber treated?
A: The Boyden Chamber Insert with 8.0 µm polyethylene terephthalate (PET) membrane is tissue-cuture treated. For cell invasion assays, this chamber can be treated with extracellular matrix components Laminin I, Collagen I, Collagen IV, and Basement Membrane Extract (BME), Regular and PathClear®. Complete cell invasion assays are available for each of these ECM components.
-
Q: The kit comes with both black and clear microplates. Do I need to use both?
A: The entire assay may be conducted in either the clear or black assay plate (provided in3455-096-01), if desired. The black assay plate provides increased sensitivity and reduced background for plate readers that read from the top. The clear assay plate provides compatibility for plate readers that read from the bottom. Some researchers have used the clear assay plate for the invasion step and the black assay plate for detection (top read).
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