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Cultrex HA-R-Spondin1-Fc 293T Cells

3710-001-01 8 Citations 2 Reviews Datasheet / COA / SDS
1 vial (1x10^6 cell/vial)
Catalog # Availability Size / Price Qty
3710-001-01
Product Details
Citations (8)
FAQs
Reviews (2)
Summary Product Datasheets Background

Cultrex HA-R-Spondin1-Fc 293T Cells Summary

Stably transfected 293T cells expressing murine R-Spondin 1 that have an N-terminal HA epitope tag and are fused to a C-terminal murine IgG2a Fc fragment.

Why Use Cultrex HA-R-Spondin1-Fc 293T Cells?

Roof plate-specific Spondin-1, (R-Spondin 1, RSPO1, or CRISTIN3), is a 27 kDa secreted activator protein that belongs to the R-Spondin family. R-Spondin positively regulates Wnt/beta-catenin signaling by acting as a ligand for Lgr4-6 receptors and as an inhibitor for ZNRF3. R-Spondin 1 induces proliferation of intestinal crypt epithelial cells, increases intestinal epithelial healing, and supports intestinal epithelial stem cell renewal. Cultrex HA-R-Spondin 1-Fc 293T Cells are stably transfected to express murine R-Spondin 1 that contains an N-terminal HA epitope tag and is fused to a C-terminal murine IgG2a Fc fragment.

Cultrex HA-R-Spondin 1-Fc 293T cell line can be used to produce either purified R-Spondin 1 or R-Spondin 1-conditioned media. The murine R-Spondin 1 protein produced from this cell line has been used extensively in organoid culture to maintain Lgr5+ stem cells. The Fc and HA tags can be used for protein purification or characterization.

Kit Contents

• 1 vial (1x10^6 cell/vial) of Cultrex HA-R-Spondin1-Fc 293T Cells

Specifications

Stability
Immediately thaw for use or, for long term storage, place in vapor phase of liquid nitrogen.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store in liquid nitrogen for up to 12 months from the date of receipt.
Species
Mouse

Limitations

For research use only. Not for diagnostic use.

Product Datasheets

Product Datasheet
COA
SDS

Citations for Cultrex HA-R-Spondin1-Fc 293T Cells

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Human gastrointestinal epithelia of the esophagus, stomach, and duodenum resolved at single-cell resolution
    Authors: GA Busslinger, BLA Weusten, A Bogte, H Begthel, LAA Brosens, H Clevers
    Cell Reports, 2021;34(10):108819.  2021
  2. Generation of Differentiating and Long-Living Intestinal Organoids Reflecting the Cellular Diversity of Canine Intestine
    Authors: N Kramer, B Pratscher, AMC Meneses, W Tschulenk, I Walter, A Swoboda, HS Kruitwagen, K Schneeberg, LC Penning, B Spee, M Kieslinger, S Brandt, IA Burgener
    Cells, 2020;9(4):.  2020
  3. Short-term organoid culture for drug sensitivity testing of high-grade serous carcinoma
    Authors: H Chen, K Gotimer, C De Souza, CG Tepper, AN Karnezis, GS Leiserowit, J Chien, LH Smith
    Gynecol. Oncol., 2020;0(0):.  2020
  4. Distinct Mesenchymal Cell Populations Generate the Essential Intestinal BMP Signaling Gradient
    Authors: N McCarthy, E Manieri, EE Storm, A Saadatpour, AM Luoma, VN Kapoor, S Madha, LT Gaynor, C Cox, S Keerthivas, K Wucherpfen, GC Yuan, FJ de Sauvage, SJ Turley, RA Shivdasani
    Cell Stem Cell, 2020;0(0):.  2020
  5. Direct therapeutic targeting of immune checkpoint PD-1 in pancreatic cancer
    Authors: M Gao, M Lin, RA Moffitt, MA Salazar, J Park, J Vacirca, C Huang, KR Shroyer, M Choi, GV Georgakis, AR Sasson, MA Talamini, J Kim
    Br. J. Cancer, 2019;120(1):88-96.  2019
  6. Paneth cell granule dynamics on secretory responses to bacterial stimuli in enteroids
    Authors: Y Yokoi, K Nakamura, T Yoneda, M Kikuchi, R Sugimoto, Y Shimizu, T Ayabe
    Sci Rep, 2019;9(1):2710.  2019
  7. Development of Collagen-Based 3D Matrix for Gastrointestinal Tract-Derived Organoid Culture
    Authors: JH Jee, DH Lee, J Ko, S Hahn, SY Jeong, HK Kim, E Park, SY Choi, S Jeong, JW Lee, HJ Cho, MS Kwon, J Yoo
    Stem Cells Int, 2019;2019(0):8472712.  2019
  8. Butyric Acid and Leucine Induce alpha-Defensin Secretion from Small Intestinal Paneth Cells
    Authors: A Takakuwa, K Nakamura, M Kikuchi, R Sugimoto, S Ohira, Y Yokoi, T Ayabe
    Nutrients, 2019;11(11):.  2019

FAQs

  1. Murine R-Spondin 1 is reported to be a 27kDa protein, but the protein produced by the 293T cells in Catalog # 3710-001-01  is 70-75 kDa. Why?

    • The natural R-Spondin 1 protein is approximately 27 kDa. The cells in this product express a construct containing both an additional HA-tag plus an Fc fragment, hence the much larger size.

  2. For culturing and handling of the Cultrex HA-R-Spondin1-Fc 293T Cells (catalog # 3710-001-01), does the Fetal Bovine Serum need to be heat-inactivated in the Basal Growth Medium or Freeze Medium?

    • Heat-inactivated serum is not required for either of these media.

  3. Has this cell line been tested for mycoplasma?

    • Yes, all retail lots are tested to confirm mycoplasma is undetected by PCR, which is documented on the lot-specific CoA.

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Reviews for Cultrex HA-R-Spondin1-Fc 293T Cells

Average Rating: 5 (Based on 2 Reviews)

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Cultrex HA-R-Spondin1-Fc 293T Cells
By Anonymous on 12/28/2020
Application:

For organoid culture

Serum Cultrex HA-R-Spondin1-Fc 293T Cells 3710-001-01
Cultrex HA-R-Spondin1-Fc 293T Cells
By Anonymous on 02/05/2019
Application: generation of organoids for in vivo studies
Reason for Rating: Cells produced R-spondin

Used to confirm activation of Wnt signalling