Cultrex HA-R-Spondin1-Fc 293T Cells
Cultrex HA-R-Spondin1-Fc 293T Cells Summary
Why Use Cultrex HA-R-Spondin1-Fc 293T Cells?
Roof plate-specific Spondin-1, (R-Spondin 1, RSPO1, or CRISTIN3), is a 27 kDa secreted activator protein that belongs to the R-Spondin family. R-Spondin positively regulates Wnt/beta-catenin signaling by acting as a ligand for Lgr4-6 receptors and as an inhibitor for ZNRF3. R-Spondin 1 induces proliferation of intestinal crypt epithelial cells, increases intestinal epithelial healing, and supports intestinal epithelial stem cell renewal. Cultrex HA-R-Spondin 1-Fc 293T Cells are stably transfected to express murine R-Spondin 1 that contains an N-terminal HA epitope tag and is fused to a C-terminal murine IgG2a Fc fragment.
Cultrex HA-R-Spondin 1-Fc 293T cell line can be used to produce either purified R-Spondin 1 or
R-Spondin 1-conditioned media. The murine R-Spondin 1 protein produced from this cell line has been used extensively in organoid culture to maintain Lgr5+ stem cells. The Fc and HA tags can be used for protein purification or characterization.
Kit Contents
• 1 vial (1x10^6 cell/vial) of Cultrex HA-R-Spondin1-Fc 293T Cells
Specifications
Limitations
For research use only. Not for diagnositic use.
Product Datasheets
Citations for Cultrex HA-R-Spondin1-Fc 293T Cells
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Generation of Differentiating and Long-Living Intestinal Organoids Reflecting the Cellular Diversity of Canine Intestine
Authors: N Kramer, B Pratscher, AMC Meneses, W Tschulenk, I Walter, A Swoboda, HS Kruitwagen, K Schneeberg, LC Penning, B Spee, M Kieslinger, S Brandt, IA Burgener
Cells, 2020;9(4):. 2020
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Short-term organoid culture for drug sensitivity testing of high-grade serous carcinoma
Authors: H Chen, K Gotimer, C De Souza, CG Tepper, AN Karnezis, GS Leiserowit, J Chien, LH Smith
Gynecol. Oncol., 2020;0(0):. 2020
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Direct therapeutic targeting of immune checkpoint PD-1 in pancreatic cancer
Authors: M Gao, M Lin, RA Moffitt, MA Salazar, J Park, J Vacirca, C Huang, KR Shroyer, M Choi, GV Georgakis, AR Sasson, MA Talamini, J Kim
Br. J. Cancer, 2019;120(1):88-96. 2019
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Paneth cell granule dynamics on secretory responses to bacterial stimuli in enteroids
Authors: Y Yokoi, K Nakamura, T Yoneda, M Kikuchi, R Sugimoto, Y Shimizu, T Ayabe
Sci Rep, 2019;9(1):2710. 2019
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Development of Collagen-Based 3D Matrix for Gastrointestinal Tract-Derived Organoid Culture
Authors: JH Jee, DH Lee, J Ko, S Hahn, SY Jeong, HK Kim, E Park, SY Choi, S Jeong, JW Lee, HJ Cho, MS Kwon, J Yoo
Stem Cells Int, 2019;2019(0):8472712. 2019
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Butyric Acid and Leucine Induce alpha-Defensin Secretion from Small Intestinal Paneth Cells
Authors: A Takakuwa, K Nakamura, M Kikuchi, R Sugimoto, S Ohira, Y Yokoi, T Ayabe
Nutrients, 2019;11(11):. 2019
FAQs
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Murine R-Spondin 1 is reported to be a 27kDa protein, but the protein produced by the 293T cells in Catalog # 3710-001-01 is 70-75 kDa. Why?
The natural R-Spondin 1 protein is approximately 27 kDa. The cells in this product express a construct containing both an additional HA-tag plus an Fc fragment, hence the much larger size.
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For culturing and handling of the Cultrex HA-R-Spondin1-Fc 293T Cells (catalog # 3710-001-01), does the Fetal Bovine Serum need to be heat-inactivated in the Basal Growth Medium or Freeze Medium?
Heat-inactivated serum is not required for either of these media.
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Has this cell line been tested for mycoplasma?
Yes, all retail lots are tested to confirm mycoplasma is undetected by PCR, which is documented on the lot-specific CoA.
Reviews for Cultrex HA-R-Spondin1-Fc 293T Cells
Average Rating: 5 (Based on 2 Reviews)
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Reason for Rating: Cells produced R-spondin
Used to confirm activation of Wnt signalling