Cultrex HA-R-Spondin1-Fc 293T Cells
Cultrex HA-R-Spondin1-Fc 293T Cells Summary
Why Use Cultrex HA-R-Spondin1-Fc 293T Cells?
Roof plate-specific Spondin-1, (R-Spondin 1, RSPO1, or CRISTIN3), is a 27 kDa secreted activator protein that belongs to the R-Spondin family. R-Spondin positively regulates Wnt/beta-catenin signaling by acting as a ligand for Lgr4-6 receptors and as an inhibitor for ZNRF3. R-Spondin 1 induces proliferation of intestinal crypt epithelial cells, increases intestinal epithelial healing, and supports intestinal epithelial stem cell renewal. Cultrex HA-R-Spondin 1-Fc 293T Cells are stably transfected to express murine R-Spondin 1 that contains an N-terminal HA epitope tag and is fused to a C-terminal murine IgG2a Fc fragment.
Cultrex HA-R-Spondin 1-Fc 293T cell line can be used to produce either purified R-Spondin 1 or R-Spondin 1-conditioned media. The murine R-Spondin 1 protein produced from this cell line has been used extensively in organoid culture to maintain Lgr5+ stem cells. The Fc and HA tags can be used for protein purification or characterization.
• 1 vial (1x10^6 cell/vial) of Cultrex HA-R-Spondin1-Fc 293T Cells
For research use only. Not for diagnositic use.
Citations for Cultrex HA-R-Spondin1-Fc 293T Cells
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 6
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Generation of Differentiating and Long-Living Intestinal Organoids Reflecting the Cellular Diversity of Canine Intestine
Authors: N Kramer, B Pratscher, AMC Meneses, W Tschulenk, I Walter, A Swoboda, HS Kruitwagen, K Schneeberg, LC Penning, B Spee, M Kieslinger, S Brandt, IA Burgener
Cells, 2020;9(4):. 2020
Short-term organoid culture for drug sensitivity testing of high-grade serous carcinoma
Authors: H Chen, K Gotimer, C De Souza, CG Tepper, AN Karnezis, GS Leiserowit, J Chien, LH Smith
Gynecol. Oncol., 2020;0(0):. 2020
Direct therapeutic targeting of immune checkpoint PD-1 in pancreatic cancer
Authors: M Gao, M Lin, RA Moffitt, MA Salazar, J Park, J Vacirca, C Huang, KR Shroyer, M Choi, GV Georgakis, AR Sasson, MA Talamini, J Kim
Br. J. Cancer, 2019;120(1):88-96. 2019
Paneth cell granule dynamics on secretory responses to bacterial stimuli in enteroids
Authors: Y Yokoi, K Nakamura, T Yoneda, M Kikuchi, R Sugimoto, Y Shimizu, T Ayabe
Sci Rep, 2019;9(1):2710. 2019
Development of Collagen-Based 3D Matrix for Gastrointestinal Tract-Derived Organoid Culture
Authors: JH Jee, DH Lee, J Ko, S Hahn, SY Jeong, HK Kim, E Park, SY Choi, S Jeong, JW Lee, HJ Cho, MS Kwon, J Yoo
Stem Cells Int, 2019;2019(0):8472712. 2019
Butyric Acid and Leucine Induce alpha-Defensin Secretion from Small Intestinal Paneth Cells
Authors: A Takakuwa, K Nakamura, M Kikuchi, R Sugimoto, S Ohira, Y Yokoi, T Ayabe
Nutrients, 2019;11(11):. 2019
Murine R-Spondin 1 is reported to be a 27kDa protein, but the protein produced by the 293T cells in Catalog # 3710-001-01 is 70-75 kDa. Why?
The natural R-Spondin 1 protein is approximately 27 kDa. The cells in this product express a construct containing both an additional HA-tag plus an Fc fragment, hence the much larger size.
For culturing and handling of the Cultrex HA-R-Spondin1-Fc 293T Cells (catalog # 3710-001-01), does the Fetal Bovine Serum need to be heat-inactivated in the Basal Growth Medium or Freeze Medium?
Heat-inactivated serum is not required for either of these media.
Has this cell line been tested for mycoplasma?
Yes, all retail lots are tested to confirm mycoplasma is undetected by PCR, which is documented on the lot-specific CoA.
Reviews for Cultrex HA-R-Spondin1-Fc 293T Cells
Average Rating: 5 (Based on 2 Reviews)
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Reason for Rating: Cells produced R-spondin
Used to confirm activation of Wnt signalling