Cultrex HA-R-Spondin1-Fc 293T Cells

R&D Systems | Catalog # 3710-001-01

1 vial (1x10^6 cell/vial)
R&D Systems
Discontinued Product
3710-001-01 has been discontinued. View all Pluripotent Stem Cells products.

Key Product Details

Features

Stably transfected 293T cells expressing murine R-Spondin 1 that have an N-terminal HA epitope tag and are fused to a C-terminal murine IgG2a Fc fragment.

Species

Mouse

Product Summary for Cultrex HA-R-Spondin1-Fc 293T Cells

Why Use Cultrex HA-R-Spondin1-Fc 293T Cells?
Roof plate-specific Spondin-1, (R-Spondin 1, RSPO1, or CRISTIN3), is a 27 kDa secreted activator protein that belongs to the R-Spondin family. R-Spondin positively regulates Wnt/beta-catenin signaling by acting as a ligand for Lgr4-6 receptors and as an inhibitor for ZNRF3. R-Spondin 1 induces proliferation of intestinal crypt epithelial cells, increases intestinal epithelial healing, and supports intestinal epithelial stem cell renewal. Cultrex HA-R-Spondin 1-Fc 293T Cells are stably transfected to express murine R-Spondin 1 that contains an N-terminal HA epitope tag and is fused to a C-terminal murine IgG2a Fc fragment.

Cultrex HA-R-Spondin 1-Fc 293T cell line can be used to produce either purified R-Spondin 1 or R-Spondin 1-conditioned media. The murine R-Spondin 1 protein produced from this cell line has been used extensively in organoid culture to maintain Lgr5+ stem cells. The Fc and HA tags can be used for protein purification or characterization.

Kit Contents for Cultrex HA-R-Spondin1-Fc 293T Cells

  • 1 vial (1x10^6 cell/vial) of Cultrex HA-R-Spondin1-Fc 293T Cells

Formulation, Preparation, and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.

Storage

Store in liquid nitrogen for up to 12 months from the date of receipt.

Stability

Immediately thaw for use or, for long term storage, place in vapor phase of liquid nitrogen.

Background: Pluripotent Stem Cells

Embryonic stem (ES) cells are pluripotent stem cells derived from the inner cell mass of pre-implantation embryos. Induced pluripotent stem (iPS) cells can be generated by somatic cell reprogramming following the exogenous expression of specific transcription factors (Oct-3/4, KLF4, SOX2, and c-Myc). These cell types are capable of unlimited, undifferentiated proliferation in vitro and still maintain the capacity to differentiate into a wide variety of somatic cells. In this capacity, pluripotent stem cells have widespread clinical potential for the treatments of heart disease, diabetes, spinal cord injury, and a variety of neurodegenerative disorders.

R&D Systems offers a wide range of products to support pluripotent stem cell culture and differentiation. Mouse embryonic fibroblasts may be used to maintain and expand pluripotent stem cells in an undifferentiated state. We also offer defined culture media, which are specifically optimized for use with human or rodent pluripotent stem cells. In addition, R&D Systems offers a variety of products to assess differentiation status and identify specific stem cell types of interest, including panels of marker antibodies, primer pairs, multi-color flow cytometry kits, and specialized verification kits.

Additional Pluripotent Stem Cells Products

Product Documents for Cultrex HA-R-Spondin1-Fc 293T Cells

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Citations for Cultrex HA-R-Spondin1-Fc 293T Cells

Customer Reviews for Cultrex HA-R-Spondin1-Fc 293T Cells (2)

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  • Cultrex HA-R-Spondin1-Fc 293T Cells
    Name: Anonymous
    Verified Customer | Posted 12/28/2020
    For organoid culture
    Cultrex HA-R-Spondin1-Fc 293T Cells 3710-001-01
  • Cultrex HA-R-Spondin1-Fc 293T Cells
    Name: Anonymous
    Application: generation of organoids for in vivo studies
    Verified Customer | Posted 02/05/2019
    Used to confirm activation of Wnt signalling

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FAQs for Cultrex HA-R-Spondin1-Fc 293T Cells

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  • Q: For culturing and handling of the Cultrex HA-R-Spondin1-Fc 293T Cells (catalog # 3710-001-01), does the Fetal Bovine Serum need to be heat-inactivated in the Basal Growth Medium or Freeze Medium?

    A: Heat-inactivated serum is not required for either of these media.

  • Q: Has this cell line been tested for mycoplasma?

    A: Yes, all retail lots are tested to confirm mycoplasma is undetected by PCR, which is documented on the lot-specific CoA.

  • Q: Murine R-Spondin 1 is reported to be a 27kDa protein, but the protein produced by the 293T cells in Catalog # 3710-001-01  is 70-75 kDa. Why?

    A: The natural R-Spondin 1 protein is approximately 27 kDa. The cells in this product express a construct containing both an additional HA-tag plus an Fc fragment, hence the much larger size.

  • Q: For culturing and handling of the Cultrex HA-R-Spondin1-Fc 293T Cells (catalog # 3710-001-01), does the Fetal Bovine Serum need to be heat-inactivated in the Basal Growth Medium or Freeze Medium?

    A: Heat-inactivated serum is not required for either of these media.

  • Q: Has this cell line been tested for mycoplasma?

    A: Yes, all retail lots are tested to confirm mycoplasma is undetected by PCR, which is documented on the lot-specific CoA.

  • Q: Murine R-Spondin 1 is reported to be a 27kDa protein, but the protein produced by the 293T cells in Catalog # 3710-001-01  is 70-75 kDa. Why?

    A: The natural R-Spondin 1 protein is approximately 27 kDa. The cells in this product express a construct containing both an additional HA-tag plus an Fc fragment, hence the much larger size.

  • Q: For culturing and handling of the Cultrex HA-R-Spondin1-Fc 293T Cells (catalog # 3710-001-01), does the Fetal Bovine Serum need to be heat-inactivated in the Basal Growth Medium or Freeze Medium?

    A: Heat-inactivated serum is not required for either of these media.

  • Q: Has this cell line been tested for mycoplasma?

    A: Yes, all retail lots are tested to confirm mycoplasma is undetected by PCR, which is documented on the lot-specific CoA.

  • Q: Murine R-Spondin 1 is reported to be a 27kDa protein, but the protein produced by the 293T cells in Catalog # 3710-001-01  is 70-75 kDa. Why?

    A: The natural R-Spondin 1 protein is approximately 27 kDa. The cells in this product express a construct containing both an additional HA-tag plus an Fc fragment, hence the much larger size.

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