Cultrex In Vitro Angiogenesis Assay (Tube Formation) 96 well
Cultrex In Vitro Angiogenesis Assay (Tube Formation) 96 well Summary
Cultrex® In Vitro Angiogenesis Assay Tube Formation Kit
The Cultrex In Vitro Angiogenesis Assay Tube Formation Kit (Catalog # 3470-096-K) provides a high-throughput screening platform to test the effects of multiple compounds on endothelial cell tube formation. This kit quantitatively assesses the ability of endothelial cells to form a tube network in a reconstituted protein matrix. The utilization of a fluorescent dye provides data on cell viability and tube network formation in real time.
Features of the Cultrex In Vitro Angiogenesis Assay Tube Formation Kit
- Allows for the detection of inducers and inhibitors of endothelial cell tube formation
- Includes reagents to rapidly and accurately monitor tube formation in real time as well as an inhibitor reagent for control experiments
- Reduced Growth Factor Basement Membrane Extract
- Calcein AM
- Cell Staining Solution
- Sulforaphane, 10 mM
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Detection of Angiogenesis Tube Formation. HUVECs were cultured on Reduced Growth Factor-Basement Membrane Extract (RGF-BME) in reduced growth factor-containing media (A), growth factor-supplemented media (B), or growth factor-supplemented media in the presence of sulforaphane, an inhibitor of angiogenesis (C). Angiogenic tube formation was assessed using the Cultrex In Vitro Angiogenesis Assay Tube Formation Kit (Catalog # 3470-096-K). Cells were loaded with Calcein AM, and cell viability and tube formation were detected by fluorescence microscopy.
Angiogenesis is the tightly regulated process by which new blood vessels are formed from the existing vasculature. This process is physiologically important for development and wound healing, and is also a common driver in multiple diseases including rheumatoid arthritis, atherosclerosis, macular degeneration, and cancer. Angiogenesis occurs in response to a variety of molecular cues. Generally, the angiogenic process includes endothelial cell proliferation, chemotactic endothelial cell migration through the extracellular matrix barrier, and the formation of capillary tubes. Physiological and pathological angiogenesis utilize many of the same cellular processes and molecular signaling networks, however the structures that form during pathological angiogenesis are often functionally abnormal.
Citations for Cultrex In Vitro Angiogenesis Assay (Tube Formation) 96 well
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Tumour exosomal CEMIP protein promotes cancer cell colonization in brain metastasis
Authors: G Rodrigues, A Hoshino, CM Kenific, IR Matei, L Steiner, D Freitas, HS Kim, PR Oxley, I Scandariat, I Casanova-S, J Dai, CR Badwe, B Gril, M Teši? Mark, BD Dill, H Molina, H Zhang, A Benito-Mar, L Bojmar, Y Ararso, K Offer, Q LaPlant, W Buehring, H Wang, X Jiang, TM Lu, Y Liu, JK Sabari, SJ Shin, N Narula, PS Ginter, VK Rajasekhar, JH Healey, E Meylan, B Costa-Silv, SE Wang, S Rafii, NK Altorki, CM Rudin, DR Jones, PS Steeg, H Peinado, CM Ghajar, J Bromberg, M de Sousa, D Pisapia, D Lyden
Nat. Cell Biol., 2019;21(11):1403-1412. 2019
The role of NUDT21 in microRNA-binging sites of EZH2 gene increases the of risk preeclampsia
Authors: X Lang, W Zhao, D Huang, W Liu, H Shen, L Xu, S Xu, Y Huang, W Cheng
J. Cell. Mol. Med., 2019;0(0):. 2019
Isoliquiritin Apioside Suppresses in vitro Invasiveness and Angiogenesis of Cancer Cells and Endothelial Cells
Authors: A Kim, JY Ma
Front Pharmacol, 2018;9(0):1455. 2018
Interleukin-33 modulates inflammation in endometriosis
Authors: JE Miller, SP Monsanto, SH Ahn, K Khalaj, AT Fazleabas, SL Young, BA Lessey, M Koti, C Tayade
Sci Rep, 2017;7(1):17903. 2017
Gastric tumour-derived ANGPT2 regulation by DARPP-32 promotes angiogenesis.
Authors: Chen Z, Zhu S, Hong J, Soutto M, Peng D, Belkhiri A, Xu Z, El-Rifai W
Gut, 0;65(6):925-34. 0
Syndecan-4 is a major syndecan in primary human endothelial cells in vitro, modulated by inflammatory stimuli and involved in wound healing.
Authors: Vuong T, Reine T, Sudworth A, Jenssen T, Kolset S
J Histochem Cytochem, 0;63(4):280-92. 0
Inhibiting NF-kappaB in the developing lung disrupts angiogenesis and alveolarization.
Authors: Iosef C, Alastalo T, Hou Y, Chen C, Adams E, Lyu S, Cornfield D, Alvira C
Am J Physiol Lung Cell Mol Physiol, 0;302(10):L1023-36. 0
What is the Tube Formation Assay?
The Tube Formation Assay is based on the ability of endothelial cells to form three-dimensional capillary-like tubular structures when cultured on a hydrogel of reconstituted basement membrane, such as Cultrex Basement Membrane Extract (BME).
What are the advantages of the Tube Formation Assay?
The Tube Formation Assay is the most widely used in vitro angiogenesis assay. The assay is rapid, inexpensive and quantifiable. It can be used to identify potentially angiogenic and anti-angiogenic factors, to determine endothelial cell phenotype, and to study pathways and mechanisms involved in angiogenesis. It can be performed in a high throughput mode to screen for a large number of compounds.
What cell types can be used in the Tube Formation Assay?
The Tube Formation Assay is specific for endothelial cells, either primary cells or immortalized cell lines. Only endothelial cells form capillary-like structures with a lumen inside. Other endothelial cell types form other structures.
What are the variables associated with the Tube Formation Assay?
The major variables associated with tube formation are composition of the Cultrex Basement Membrane Extract (BME) hydrogel, thickness of the hydrogel, cell density, composition of angiogenic factors in the assay medium, and assay period.
How do I reduce spontaneous formation of tubular structures on Cultrex BME in the absence of angiogenic factors?
Primary endothelial cells, such as Human Umbilical Vein Endothelial Cells (HUVECs) form capillary-like structures in the absence of added angiogenic factors less often than immortalized endothelial cells. Generally, reducing the number of cells per cm2 plated onto Cultrex BME will result in less background or spontaneous tube formation. Titrate the number of cells and find optimal conditions for your specific cell line. When endothelial cells fully form capillary structures in response to angiogenic activators, but not in their absence, you may proceed.
Which Cultrex Basement Membrane Extract (BME) should I use for the Tube Formation Assay?
Cultrex Reduced Growth Factor BME (RGF BME) is generally used for testing compounds that promote angiogenesis because formation of capillary-like structures (tubes) is significantly less compared to non-growth factor reduced varieties of Cultrex BME. The Cultrex In Vitro Angiogeneis Assay (Tube Formation) includes a qualified production lot of Cultrex RGF BME that exhibits reduced background tube formation in the absence of angiogenic factors.
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