Cultrex In Vitro Angiogenesis Assay (Tube Formation) 96 well
Cultrex In Vitro Angiogenesis Assay (Tube Formation) 96 well Summary
Cultrex® In Vitro Angiogenesis Assay Tube Formation Kit
The Cultrex In Vitro Angiogenesis Assay Tube Formation Kit (Catalog # 3470-096-K) provides a high-throughput screening platform to test the effects of multiple compounds on endothelial cell tube formation. This kit quantitatively assesses the ability of endothelial cells to form a tube network in a reconstituted protein matrix. The utilization of a fluorescent dye provides data on cell viability and tube network formation in real time.
Features of the Cultrex In Vitro Angiogenesis Assay Tube Formation Kit
- Allows for the detection of inducers and inhibitors of endothelial cell tube formation
- Includes reagents to rapidly and accurately monitor tube formation in real time as well as an inhibitor reagent for control experiments
- Reduced Growth Factor Basement Membrane Extract
- Calcein AM
- Cell Staining Solution
- Sulforaphane, 10 mM
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Detection of Angiogenesis Tube Formation. HUVECs were cultured on Reduced Growth Factor-Basement Membrane Extract (RGF-BME) in reduced growth factor-containing media (A), growth factor-supplemented media (B), or growth factor-supplemented media in the presence of sulforaphane, an inhibitor of angiogenesis (C). Angiogenic tube formation was assessed using the Cultrex In Vitro Angiogenesis Assay Tube Formation Kit (Catalog # 3470-096-K). Cells were loaded with Calcein AM, and cell viability and tube formation were detected by fluorescence microscopy.
Preparation and Storage
Background: Angiogenesis Assay Kits
Angiogenesis is the tightly regulated process by which new blood vessels are formed from the existing vasculature. This process is physiologically important for development and wound healing, and is also a common driver in multiple diseases including rheumatoid arthritis, atherosclerosis, macular degeneration, and cancer. Angiogenesis occurs in response to a variety of molecular cues. Generally, the angiogenic process includes endothelial cell proliferation, chemotactic endothelial cell migration through the extracellular matrix barrier, and the formation of capillary tubes. Physiological and pathological angiogenesis utilize many of the same cellular processes and molecular signaling networks, however the structures that form during pathological angiogenesis are often functionally abnormal.
Citations for Cultrex In Vitro Angiogenesis Assay (Tube Formation) 96 well
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Citations: Showing 1 - 5
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Isoliquiritin Apioside Suppresses in vitro Invasiveness and Angiogenesis of Cancer Cells and Endothelial Cells
Authors: A Kim, JY Ma
Front Pharmacol, 2018;9(0):1455. 2018
Interleukin-33 modulates inflammation in endometriosis
Authors: JE Miller, SP Monsanto, SH Ahn, K Khalaj, AT Fazleabas, SL Young, BA Lessey, M Koti, C Tayade
Sci Rep, 2017;7(1):17903. 2017
Gastric tumour-derived ANGPT2 regulation by DARPP-32 promotes angiogenesis.
Authors: Chen Z, Zhu S, Hong J, Soutto M, Peng D, Belkhiri A, Xu Z, El-Rifai W
Gut, 0;65(6):925-34. 0
Syndecan-4 is a major syndecan in primary human endothelial cells in vitro, modulated by inflammatory stimuli and involved in wound healing.
Authors: Vuong T, Reine T, Sudworth A, Jenssen T, Kolset S
J Histochem Cytochem, 0;63(4):280-92. 0
Inhibiting NF-kappaB in the developing lung disrupts angiogenesis and alveolarization.
Authors: Iosef C, Alastalo T, Hou Y, Chen C, Adams E, Lyu S, Cornfield D, Alvira C
Am J Physiol Lung Cell Mol Physiol, 0;302(10):L1023-36. 0
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