Cultrex In Vitro Angiogenesis Assay (Tube Formation) 96 well

Catalog # Availability Size / Price Qty
3470-096-K
Product Details
Citations (6)
FAQs
Reviews (3)

Cultrex In Vitro Angiogenesis Assay (Tube Formation) 96 well Summary

Cultrex® In Vitro Angiogenesis Assay Tube Formation Kit

The Cultrex In Vitro Angiogenesis Assay Tube Formation Kit (Catalog # 3470-096-K) provides a high-throughput screening platform to test the effects of multiple compounds on endothelial cell tube formation. This kit quantitatively assesses the ability of endothelial cells to form a tube network in a reconstituted protein matrix. The utilization of a fluorescent dye provides data on cell viability and tube network formation in real time.

Features of the Cultrex In Vitro Angiogenesis Assay Tube Formation Kit

  • Allows for the detection of inducers and inhibitors of endothelial cell tube formation
  • Includes reagents to rapidly and accurately monitor tube formation in real time as well as an inhibitor reagent for control experiments

Kit Contents

  • Reduced Growth Factor Basement Membrane Extract
  • Calcein AM
  • Cell Staining Solution
  • Sulforaphane, 10 mM
 

Data Examples

Detection of Angiogenesis Tube Formation
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Detection of Angiogenesis Tube Formation. HUVECs were cultured on Reduced Growth Factor-Basement Membrane Extract (RGF-BME) in reduced growth factor-containing media (A), growth factor-supplemented media (B), or growth factor-supplemented media in the presence of sulforaphane, an inhibitor of angiogenesis (C). Angiogenic tube formation was assessed using the Cultrex In Vitro Angiogenesis Assay Tube Formation Kit (Catalog # 3470-096-K). Cells were loaded with Calcein AM, and cell viability and tube formation were detected by fluorescence microscopy. 

Product Datasheets

Preparation and Storage

Stability & Storage
The product requires storage at -20 to -70 °C and room temperature.  Consult the product insert for specific storage temperatures. Do not use past expiration date.

Background: Angiogenesis Assay Kits

Angiogenesis is the tightly regulated process by which new blood vessels are formed from the existing vasculature. This process is physiologically important for development and wound healing, and is also a common driver in multiple diseases including rheumatoid arthritis, atherosclerosis, macular degeneration, and cancer. Angiogenesis occurs in response to a variety of molecular cues. Generally, the angiogenic process includes endothelial cell proliferation, chemotactic endothelial cell migration through the extracellular matrix barrier, and the formation of capillary tubes. Physiological and pathological angiogenesis utilize many of the same cellular processes and molecular signaling networks, however the structures that form during pathological angiogenesis are often functionally abnormal.

Alternate Names
Angiogenesis Assay Kits

Citations for Cultrex In Vitro Angiogenesis Assay (Tube Formation) 96 well

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. The role of NUDT21 in microRNA-binging sites of EZH2 gene increases the of risk preeclampsia
    Authors: X Lang, W Zhao, D Huang, W Liu, H Shen, L Xu, S Xu, Y Huang, W Cheng
    J. Cell. Mol. Med., 2019;0(0):.  2019
  2. Isoliquiritin Apioside Suppresses in vitro Invasiveness and Angiogenesis of Cancer Cells and Endothelial Cells
    Authors: A Kim, JY Ma
    Front Pharmacol, 2018;9(0):1455.  2018
  3. Interleukin-33 modulates inflammation in endometriosis
    Authors: JE Miller, SP Monsanto, SH Ahn, K Khalaj, AT Fazleabas, SL Young, BA Lessey, M Koti, C Tayade
    Sci Rep, 2017;7(1):17903.  2017
  4. Gastric tumour-derived ANGPT2 regulation by DARPP-32 promotes angiogenesis.
    Authors: Chen Z, Zhu S, Hong J, Soutto M, Peng D, Belkhiri A, Xu Z, El-Rifai W
    Gut, 0;65(6):925-34.  0
  5. Syndecan-4 is a major syndecan in primary human endothelial cells in vitro, modulated by inflammatory stimuli and involved in wound healing.
    Authors: Vuong T, Reine T, Sudworth A, Jenssen T, Kolset S
    J Histochem Cytochem, 0;63(4):280-92.  0
  6. Inhibiting NF-kappaB in the developing lung disrupts angiogenesis and alveolarization.
    Authors: Iosef C, Alastalo T, Hou Y, Chen C, Adams E, Lyu S, Cornfield D, Alvira C
    Am J Physiol Lung Cell Mol Physiol, 0;302(10):L1023-36.  0

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Reviews for Cultrex In Vitro Angiogenesis Assay (Tube Formation) 96 well

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Cultrex In Vitro Angiogenesis Assay (Tube Formation) 96 well
By Anonymous on 05/09/2016

Cultrex In Vitro Angiogenesis Assay (Tube Formation) 96 well
By Anonymous on 05/09/2016

Cultrex In Vitro Angiogenesis Assay (Tube Formation) 96 well
By Anonymous on 05/09/2016