Laminins are heterotrimeric, noncollagenous glycoproteins composed of combinations of five known alpha, four beta and three gamma chains in mammals. One alpha, beta and gamma subunit self-assembles to form at least 15 coiled-coil heterotrimers. Through specific interactions with integrins, dystroglycan, and other receptors, laminins contribute to cell differentation, cell shape and migration, maintenance of tissue phenotypes and survival. Some subunits, such as Laminin gamma1 (Laminin B2), are present in several laminins; Laminin-1 (EHS laminin), Laminin-2 (merosin), Laminin-3 (S-laminin), Laminin-4 (S-merosin), Laminin-6 (K-laminin) and Laminin-7 (KS-laminin). Others, such as the beta3 and gamma2 subunits, occur in only one known combination (e.g. with alpha3 in Laminin-5 / Epiligrin).
Cultrex Mouse Laminin I, Pathclear
R&D Systems | Catalog # 3400-010-02
Key Product Details
Features
Key Benefits
- Produced and qualified for use in cell culture
- Pathclear-tested to ensure a pathogen and virus-free material
- Source tissue is LDEV-free
Species
Product Summary for Cultrex Mouse Laminin I, Pathclear
Negative for the presence of bacteria and fungi.
Product Specifications
Source
Protein Concentration
Endotoxin Level
Sterility Testing
Cell Culture Testing
Viral Testing
Formulation, Preparation, and Storage
Shipping
Storage
Stability
Background: Laminin-1
Alternate Names
Additional Laminin-1 Products
Product Documents for Cultrex Mouse Laminin I, Pathclear
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Related Research Areas
Citations for Cultrex Mouse Laminin I, Pathclear
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Customer Reviews for Cultrex Mouse Laminin I, Pathclear (2)
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Verified Customer | Posted 05/20/202510 µg/mL for coating in PBS (2h 37°C or 4°C overnight)
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Sample Tested: Neural progenitor cellsSpecies: MouseVerified Customer | Posted 09/16/2024I used Cultrex Mouse Laminin I, Pathclear as a coating (PLO/Laminin) on 6-well plates at a concentration of 10 µg/ml to improve the adhesion of neural progenitor cells (NPCs). The coating significantly enhanced cell attachment and supported robust growth. Additionally, I used it in the NPC culture medium at 1 µg/ml, where it further promoted cell maintenance and proliferation. This product performed exceptionally well and integrated smoothly into my cell culture protocol. Highly recommend!
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FAQs for Cultrex Mouse Laminin I, Pathclear
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Q: Can Cultrex Mouse Laminin I be used on glass cover slips?
A: Yes. Coating glass cover slips with Cultrex Laminin I is quite common and has been published extensively.
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Q: What is the recommended working concentration for Cultrex Laminin I?
A: The recommended working concentration for thin coating is 0.05-10 µg/cm2 . However, conditions must be optimized for each cell line or model. Cultrex Laminin I should be used at 6 mg/mL for 3-D culture applications.
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Q: Can Cultrex Mouse Laminin I be used on glass cover slips?
A: Yes. Coating glass cover slips with Cultrex Laminin I is quite common and has been published extensively.
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Q: What is the recommended working concentration for Cultrex Laminin I?
A: The recommended working concentration for thin coating is 0.05-10 µg/cm2 . However, conditions must be optimized for each cell line or model. Cultrex Laminin I should be used at 6 mg/mL for 3-D culture applications.