Cultrex Mouse Laminin I, Pathclear

Mouse Laminin I purified and qualified for use in cell culture
Catalog # Availability Size / Price Qty
3400-010-02
Product Details
Citations (8)
FAQs
Reviews

Cultrex Mouse Laminin I, Pathclear Summary

Cultrex Mouse Laminin I, Pathclear is a purified basement membrane protein that has been developed and qualified as a extracellular matrix coating solution for cell attachment to tissue culture vessels.

Key Benefits

• Produced and qualified for use in cell culture
• Pathclear-tested to ensure a pathogen and virus-free material
• Source tissue is LDEV-free

Why Use Cultrex Mouse Laminin I?

Cultrex Mouse Laminin I, Pathclear is purified from Engelbreth-Holm-Swarm (EHS) tumor and is Pathclear-certified to be free of common pathogens. Laminin I is a major component of the basement membrane which is a continuous sheet of specialized extracellular matrix that forms an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that plays an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation.

Specifications

Source
Murine Engelbreth-Holm-Swarm (EHS) tumor
Protein Concentration
1 mg/mL
Endotoxin Level
≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
Tested following USP <71> sterility guidelines.
Testing Cell Culture
Supports cell attachment and spreading of MG63 human osteosarcoma cells.
Viral Testing
Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.
Stability
Product is stable for a minimum of 3 months from date of shipment. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Species
Mouse

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Citations for Cultrex Mouse Laminin I, Pathclear

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Fatty acid synthase downregulation contributes to acute lung injury in murine diet-induced obesity
    Authors: M Plataki, L Fan, E Sanchez, Z Huang, LK Torres, M Imamura, Y Zhu, DE Cohen, SM Cloonan, AM Choi
    JCI Insight, 2019;5(0):.  2019
  2. Synaptogenesis Stimulates a Proteasome-Mediated Ribosome Reduction in Axons
    Authors: RO Costa, H Martins, LF Martins, AW Cwetsch, M Mele, JR Pedro, D Tomé, NL Jeon, L Cancedda, SR Jaffrey, RD Almeida
    Cell Rep, 2019;28(4):864-876.e6.  2019
  3. The effects of focal adhesion kinase and platelet-derived growth factor receptor beta inhibition in a patient-derived xenograft model of primary and metastatic Wilms tumor
    Authors: JM Aye, LL Stafman, AP Williams, EF Garner, JE Stewart, JC Anderson, S Mruthyunja, MG Waldrop, CD Goolsby, HR Markert, C Quinn, R Marayati, E Mroczek-Mu, CD Willey, KJ Yoon, KF Whelan, EA Beierle
    Oncotarget, 2019;10(53):5534-5548.  2019
  4. Focal Adhesion Kinase Inhibition Contributes to Tumor Cell Survival and Motility in Neuroblastoma Patient-Derived Xenografts
    Authors: LL Stafman, AP Williams, R Marayati, JM Aye, HR Markert, EF Garner, CH Quinn, SB Lallani, JE Stewart, KJ Yoon, K Whelan, EA Beierle
    Sci Rep, 2019;9(1):13259.  2019
  5. A cell fitness selection model for neuronal survival during development
    Authors: Y Wang, H Wu, P Fontanet, S Codeluppi, N Akkuratova, C Petitpré, Y Xue-Franzé, K Niederreit, A Sharma, F Da Silva, G Comai, G Agirman, D Palumberi, S Linnarsson, I Adameyko, A Moqrich, A Schedl, G La Manno, S Hadjab, F Lallemend
    Nat Commun, 2019;10(1):4137.  2019
  6. Mechanisms of hyperexcitability in Alzheimer's disease hiPSC-derived neurons and cerebral organoids vs. isogenic control
    Authors: S Ghatak, N Dolatabadi, D Trudler, X Zhang, Y Wu, M Mohata, R Ambasudhan, M Talantova, SA Lipton
    Elife, 2019;8(0):.  2019
  7. Dynamics of PARKIN-Dependent Mitochondrial Ubiquitylation in Induced Neurons and Model Systems Revealed by Digital Snapshot Proteomics
    Authors: A Ordureau, JA Paulo, W Zhang, T Ahfeldt, J Zhang, EF Cohn, Z Hou, JM Heo, LL Rubin, SS Sidhu, SP Gygi, JW Harper
    Mol. Cell, 2018;70(2):211-227.e8.  2018
  8. Amacrine cell subtypes differ in their intrinsic neurite growth capacity.
    Authors: Kunzevitzky N, Willeford K, Feuer W, Almeida M, Goldberg J
    Invest Ophthalmol Vis Sci, 0;54(12):7603-13.  0

FAQs

  1. What is the recommended working concentration for Cultrex Laminin I?

    • The recommended working concentration for thin coating is 0.05-10 µg/cm2 . However, conditions must be optimized for each cell line or model. Cultrex Laminin I should be used at 6 mg/mL for 3-D culture applications.

       

  2. What is Laminin I?

    • Laminin I is a major component of extracellular matrix. Cultrex Laminin I is purified from murine EHS sarcoma. It is composed of α1β1γ1 chains with a total MW of 800,000 Da. Cultrex Laminin I increases cell adhesion, migration, growth, differentiation, neurite outgrowth, protease production, and malignancy. The response is dependent on cell type.

  3. Can Cultrex Mouse Laminin I be used on glass cover slips?

    • Coating glass cover slips with Cultrex Laminin I is quite common and has been published extensively.

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