Cultrex Organoid Harvesting Solution
Cultrex Organoid Harvesting Solution Summary
Why Use Cultrex Organoid Harvesting Solution?
Organoid cultures exhibit cellular behaviors and morphologies similar to those seen in vivo, however, the adaptation of these models for studying biochemical processes has been impeded by the challenge of separating intact organoids from extra-cellular proteins comprising the hydrogel. Commonly, proteases are employed to degrade these extracellular proteins, however, proteases also degrade proteins on the cell surface and protease activity may carry over into subsequent cultures or lysate preparations. Cultrex Organoid Harvesting Solution provides a non-enzymatic method for depolymerizing extracellular matrix proteins to allow for harvesting of intact organoids for passaging, cryopreservation, or biochemical analysis.
For research use only. Not for diagnositic use.
Citations for Cultrex Organoid Harvesting Solution
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Renal expression of JAK2 is high in polycystic kidney disease and its inhibition reduces cystogenesis
Authors: F Patera, A Cudzich-Ma, Z Huang, M Fragiadaki
Sci Rep, 2019;9(1):4491. 2019
Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids
Authors: B Artegiani, L van Voorth, RGH Lindeboom, D Seinstra, I Heo, P Tapia, C López-Igle, D Postrach, T Dayton, R Oka, H Hu, R van Boxtel, JH van Es, J Offerhaus, PJ Peters, J van Rheene, M Vermeulen, H Clevers
Cell Stem Cell, 2019;0(0):. 2019
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