Cultrex Organoid Harvesting Solution

Ready-to-use, non-enzymatic organoid harvesting and dissociation solution
Catalog # Availability Size / Price Qty
3700-100-01

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Product Details
Citations (16)
FAQs
Reviews

Cultrex Organoid Harvesting Solution Summary

Ready-to-use, non-enzymatic solution to depolymerize basement membrane matrix for harvesting organoids from culture.

Why Use Cultrex Organoid Harvesting Solution?

Organoid cultures exhibit cellular behaviors and morphologies similar to those seen in vivo, however, the adaptation of these models for studying biochemical processes has been impeded by the challenge of separating intact organoids from extra-cellular proteins comprising the hydrogel. Commonly, proteases are employed to degrade these extracellular proteins, however, proteases also degrade proteins on the cell surface and protease activity may carry over into subsequent cultures or lysate preparations. Cultrex Organoid Harvesting Solution provides a non-enzymatic method for depolymerizing extracellular matrix proteins to allow for harvesting of intact organoids for passaging, cryopreservation, or biochemical analysis.

Specifications

Stability
Product is stable for at least 2 months from the date of receipt when stored at 2-8 °C. Keep sterile. See lot specific Certificate of Analysis for expiration.
Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at 2 - 8 °C.
Species
N/A

Limitations

For research use only. Not for diagnostic use.

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Citations for Cultrex Organoid Harvesting Solution

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Deficiency of N-glycanase 1 perturbs neurogenesis and cerebral development modeled by human organoids
    Authors: VJT Lin, J Hu, A Zolekar, MR Salick, P Mittal, JT Bird, P Hoffmann, A Kaykas, SD Byrum, YC Wang
    Cell Death & Disease, 2022;13(3):262.  2022
  2. An in�vitro chronic damage model impairs inflammatory and regenerative responses in human colonoid monolayers
    Authors: WD Rees, N Telkar, DTS Lin, MQ Wong, C Poloni, A Fathi, M Kobor, NC Zachos, TS Steiner
    Cell Reports, 2022;38(3):110283.  2022
  3. Comprehensive metabolomics expands precision medicine for triple-negative breast cancer
    Authors: Y Xiao, D Ma, YS Yang, F Yang, JH Ding, Y Gong, L Jiang, LP Ge, SY Wu, Q Yu, Q Zhang, F Bertucci, Q Sun, X Hu, DQ Li, ZM Shao, YZ Jiang
    Cell Research, 2022;0(0):.  2022
  4. A TMPRSS2 inhibitor acts as a pan-SARS-CoV-2 prophylactic and therapeutic
    Authors: T Shapira, IA Monreal, SP Dion, DW Buchholz, B Imbiakha, AD Olmstead, M Jager, A Désilets, G Gao, M Martins, T Vandal, CAH Thompson, A Chin, WD Rees, T Steiner, IR Nabi, E Marsault, J Sahler, DG Diel, GR Van de Wal, A August, GR Whittaker, PL Boudreault, R Leduc, HC Aguilar, F Jean
    Nature, 2022;0(0):.  2022
  5. In Situ Vitrification of Lung Cancer Organoids on a Microwell Array
    Authors: Q Liu, T Zhao, X Wang, Z Chen, Y Hu, X Chen
    Micromachines, 2021;12(6):.  2021
  6. Successful Patient-Derived Organoid Culture of Gynecologic Cancers for Disease Modeling and Drug Sensitivity Testing
    Authors: J Bi, AM Newtson, Y Zhang, EJ Devor, MI Samuelson, KW Thiel, KK Leslie
    Cancers, 2021;13(12):.  2021
  7. Tumor Extracellular Vesicles Regulate Macrophage-Driven Metastasis through CCL5
    Authors: DC Rabe, ND Walker, FD Rustandy, J Wallace, J Lee, SL Stott, MR Rosner
    Cancers, 2021;13(14):.  2021
  8. CHRONIC INFLAMMATION IN ULCERATIVE COLITIS CAUSES LONG TERM CHANGES IN GOBLET CELL FUNCTION
    Authors: S Varsha, J Kelli, Y Jianyi, L Sun, L Ruxian, Y Huimin, I Julie, FA Jennifer, ZC Nicholas, D Mark, R Yan
    Cellular and Molecular Gastroenterology and Hepatology, 2021;0(0):.  2021
  9. Lung cancer organoids analyzed on microwell arrays predict drug responses of patients within a week
    Authors: Y Hu, X Sui, F Song, Y Li, K Li, Z Chen, F Yang, X Chen, Y Zhang, X Wang, Q Liu, C Li, B Zou, X Chen, J Wang, P Liu
    Nature Communications, 2021;12(1):2581.  2021
  10. Grouped-seq for integrated phenotypic and transcriptomic screening of patient-derived tumor organoids
    Authors: Y Wu, K Li, Y Li, T Sun, C Liu, C Dong, T Zhao, D Tang, X Chen, X Chen, P Liu
    Nucleic Acids Research, 2021;0(0):.  2021
  11. Sonlicromanol's active metabolite KH176m normalizes prostate cancer stem cell mPGES-1 overexpression and inhibits cancer spheroid growth
    Authors: X Jiang, H Renkema, J Smeitink, J Beyrath
    PLoS ONE, 2021;16(7):e0254315.  2021
  12. A Human Organoid Model of Aggressive Hepatoblastoma for Disease Modeling and Drug Testing
    Authors: JA Saltsman, WJ Hammond, NJC Narayan, D Requena, H Gehart, G Lalazar, MP LaQuaglia, H Clevers, S Simon
    Cancers, 2020;12(9):.  2020
  13. The Fragile X Mental Retardation Protein regulates RIP1K and colorectal cancer resistance to necroptosis
    Authors: A Di Grazia, I Marafini, G Pedini, D Di Fusco, F Laudisi, V Dinallo, E Rosina, C Stolfi, E Franzè, P Sileri, G Sica, G Monteleone, C Bagni, I Monteleone
    Cell Mol Gastroenterol Hepatol, 2020;0(0):.  2020
  14. Human BCL-G regulates secretion of inflammatory chemokines but is dispensable for induction of apoptosis by IFN-gamma and TNF-alpha in intestinal epithelial cells
    Authors: JA Woznicki, P Flood, M Bustamante, P Stamou, G Moloney, A Fanning, SA Zulquernai, J McCarthy, F Shanahan, S Melgar, K Nally
    Cell Death Dis, 2020;11(1):68.  2020
  15. Renal expression of JAK2 is high in polycystic kidney disease and its inhibition reduces cystogenesis
    Authors: F Patera, A Cudzich-Ma, Z Huang, M Fragiadaki
    Sci Rep, 2019;9(1):4491.  2019
  16. Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids
    Authors: B Artegiani, L van Voorth, RGH Lindeboom, D Seinstra, I Heo, P Tapia, C López-Igle, D Postrach, T Dayton, R Oka, H Hu, R van Boxtel, JH van Es, J Offerhaus, PJ Peters, J van Rheene, M Vermeulen, H Clevers
    Cell Stem Cell, 2019;0(0):.  2019

FAQs

  1. Can the Organoid Harvesting Solution, Catalog # 3700-100-01,  be used to dissociate cells grown on a substrate of Cultrex Rat Collagen 1?

    • The Organoid Harvesting Solution works well with BME (Basement Membrane Extract).  For cells grown on Cultrex Rat Collagen 1, the recommended enzyme to use to dissociate cells is Collagenase. Collagenase treatment at 37oC for 30 minutes is recommended.

  2. Are Cultrex Organoid Harvesting Solution (Catalog # 3700-100-01) and dispase interchangeable?

    • Cultrex Organoid Harvesting Solution (Catalog # 3700-100-01) is a non-enzymatic solution that depolymerizes the matrix to enable harvest of organoids. In contrast, dispase is a protease enzyme that degrades the matrix. End users will need to empirically determine whether enzymatic or non-enzymatic solutions are more appropriate for their experimental setup.

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