Cultrex Organoid Harvesting Solution

20% Off ECM, Media, Buffers, and Serum with Promo Code CULTURE20! See T&C

Ready-to-use, non-enzymatic organoid harvesting and dissociation solution
Catalog # Availability Size / Price Qty
3700-100-01

SAVE on Cell Culture products with CULTURE20 (See Promo Details)

Product Details
Citations (33)
FAQs
Reviews (1)

Cultrex Organoid Harvesting Solution Summary

Ready-to-use, non-enzymatic solution to depolymerize basement membrane matrix for harvesting organoids from culture.

Why Use Cultrex Organoid Harvesting Solution?

Organoid cultures exhibit cellular behaviors and morphologies similar to those seen in vivo, however, the adaptation of these models for studying biochemical processes has been impeded by the challenge of separating intact organoids from extra-cellular proteins comprising the hydrogel. Commonly, proteases are employed to degrade these extracellular proteins, however, proteases also degrade proteins on the cell surface and protease activity may carry over into subsequent cultures or lysate preparations. Cultrex Organoid Harvesting Solution provides a non-enzymatic method for depolymerizing extracellular matrix proteins to allow for harvesting of intact organoids for passaging, cryopreservation, or biochemical analysis.

Specifications

Stability
Product is stable for at least 2 months from the date of receipt when stored at 2-8 °C. Keep sterile. See lot specific Certificate of Analysis for expiration.
Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at 2 - 8 °C.
Species
N/A

Limitations

For research use only. Not for diagnostic use.

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Citations for Cultrex Organoid Harvesting Solution

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
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  1. SIRT1 controls enteroendocrine progenitor cell proliferation in high-fat diet-fed mice
    Authors: Miura, M;Igarashi, M;Isotani, R;Nakagawa-Nagahama, Y;Kuranami, S;Naruse, K;Kadowaki, T;Yamauchi, T;
    Cellular and molecular gastroenterology and hepatology  2023-08-18
  2. Uranium-bearing dust induces differentiation and expansion of enteroendocrine cells in human colonoids
    Authors: Atanga, R;Appell, LL;Lauer, FT;Brearley, A;Campen, MJ;Castillo, EF;In, JG;
    bioRxiv : the preprint server for biology  2023-08-10
  3. Inflammatory macrophages prevent colonic goblet and enteroendocrine cell differentiation through Notch signaling
    Authors: Atanga, R;Romero, AS;Hernandez, AJ;Peralta-Herrera, E;Merkley, SD;In, JG;Castillo, EF;
    bioRxiv : the preprint server for biology  2023-06-29
  4. Patterning and folding of intestinal villi by active mesenchymal dewetting
    Authors: Huycke, TR;Miyazaki, H;Häkkinen, TJ;Srivastava, V;Barruet, E;McGinnis, CS;Kalantari, A;Cornwall-Scoones, J;Vaka, D;Zhu, Q;Jo, H;DeGrado, WF;Thomson, M;Garikipati, K;Boffelli, D;Klein, OD;Gartner, ZJ;
    bioRxiv : the preprint server for biology  2023-06-26
  5. Fibroblast-derived EGF ligand Neuregulin-1 induces fetal-like reprogramming of the intestinal epithelium without supporting tumorigenic growth
    Authors: TT Lemmetyine, EW Viitala, L Wartiovaar, T Kaprio, J Hagström, C Haglund, P Katajisto, TC Wang, E Domènech-M, S Ollila
    Disease Models & Mechanisms, 2023-04-03;0(0):.  2023-04-03
  6. Tellu: an object detector algorithm for automatic classification of intestinal organoids
    Authors: E Domènech-M, A Brandt, TT Lemmetyine, L Wartiovaar, TP Mäkelä, S Ollila
    Disease Models & Mechanisms, 2023-03-13;0(0):.  2023-03-13
  7. ONECUT2 regulates RANKL-dependent enterocyte and microfold cell differentiation in the small intestine; a multi-omics study
    Authors: MV Luna Velez, HK Neikes, RR Snabel, Y Quint, C Qian, A Martens, GJC Veenstra, MR Freeman, SJ van Heerin, M Vermeulen
    Nucleic Acids Research, 2023-02-22;0(0):.  2023-02-22
  8. Cannabidiol Decreases Intestinal Inflammation in the Ovariectomized Murine Model of Postmenopause
    Authors: KMA Bacalia, KM Tveter, H Palmer, J Douyere, S Martinez, K Sui, DE Roopchand
    Biomedicines, 2022-12-28;11(1):.  2022-12-28
  9. AKTIP loss is enriched in ERalpha-positive breast cancer for tumorigenesis and confers endocrine resistance
    Authors: ASN Ng, S Zhang, VCY Mak, Y Zhou, Y Yuen, R Sharma, Y Lu, G Zhuang, W Zhao, HH Pang, LWT Cheung
    Cell Reports, 2022-12-13;41(11):111821.  2022-12-13
  10. Long Noncoding RNA, MicroRNA, Zn Transporter Zip14 (Slc39a14) and Inflammation in Mice
    Authors: FR Jimenez-Ro, CH Ruggiero, RJ Cousins
    Nutrients, 2022-12-01;14(23):.  2022-12-01
  11. Chemical induction of gut beta-like-cells by combined FoxO1/Notch inhibition as a glucose-lowering treatment for diabetes
    Authors: T Kitamoto, YK Lee, N Sultana, H Watanabe, WM McKimpson, W Du, J Fan, B Diaz, HV Lin, RL Leibel, S Belvedere, D Accili
    Molecular Metabolism, 2022-10-28;66(0):101624.  2022-10-28
  12. Long-term maintenance of human endometrial epithelial stem cells and their therapeutic effects on intrauterine adhesion
    Authors: W He, X Zhu, A Xin, H Zhang, Y Sun, H Xu, H Li, T Yang, D Zhou, H Yan, X Sun
    Cell & bioscience, 2022-10-18;12(1):175.  2022-10-18
  13. CDC42 governs normal oviduct multiciliogenesis through activating AKT to ensure timely embryo transport
    Authors: R Jiang, X Tang, J Pan, G Li, N Yang, Y Tang, S Bi, H Cai, Q Chen, D Chen, H Wang, S Kong
    Cell Death & Disease, 2022-09-02;13(9):757.  2022-09-02
  14. The role of CFA/I in adherence and toxin delivery by ETEC expressing multiple colonization factors in the human enteroid model
    Authors: EM Smith, CL Grassel, A Papadimas, J Foulke-Abe, EM Barry
    PloS Neglected Tropical Diseases, 2022-07-26;16(7):e0010638.  2022-07-26
  15. Human liver organoids for disease modeling of fibrolamellar carcinoma
    Authors: NJC Narayan, D Requena, G Lalazar, L Ramos-Espi, D Ng, S Levin, B Shebl, R Wang, WJ Hammond, JA Saltsman, H Gehart, MS Torbenson, H Clevers, MP LaQuaglia, SM Simon
    Stem Cell Reports, 2022-07-07;17(8):1874-1888.  2022-07-07
  16. Equine Oviductal Organoid Generation and Cryopreservation
    Authors: RE Thompson, MA Meyers, DNR Veeramacha, BS Pukazhenth, FK Hollinshea
    Methods and protocols, 2022-06-15;5(3):.  2022-06-15
  17. Hesperadin suppresses pancreatic cancer through ATF4/GADD45A axis at nanomolar concentrations
    Authors: Y Zhang, J Wu, Y Fu, R Yu, H Su, Q Zheng, H Wu, S Zhou, K Wang, J Zhao, S Shen, G Xu, L Wang, C Yan, X Zou, Y Lv, S Zhang
    Oncogene, 2022-05-12;0(0):.  2022-05-12
  18. A TMPRSS2 inhibitor acts as a pan-SARS-CoV-2 prophylactic and therapeutic
    Authors: T Shapira, IA Monreal, SP Dion, DW Buchholz, B Imbiakha, AD Olmstead, M Jager, A Désilets, G Gao, M Martins, T Vandal, CAH Thompson, A Chin, WD Rees, T Steiner, IR Nabi, E Marsault, J Sahler, DG Diel, GR Van de Wal, A August, GR Whittaker, PL Boudreault, R Leduc, HC Aguilar, F Jean
    Nature, 2022-03-28;0(0):.  2022-03-28
  19. Deficiency of N-glycanase 1 perturbs neurogenesis and cerebral development modeled by human organoids
    Authors: VJT Lin, J Hu, A Zolekar, MR Salick, P Mittal, JT Bird, P Hoffmann, A Kaykas, SD Byrum, YC Wang
    Cell Death & Disease, 2022-03-24;13(3):262.  2022-03-24
  20. Grouped-seq for integrated phenotypic and transcriptomic screening of patient-derived tumor organoids
    Authors: Y Wu, K Li, Y Li, T Sun, C Liu, C Dong, T Zhao, D Tang, X Chen, X Chen, P Liu
    Nucleic Acids Research, 2022-03-21;0(0):.  2022-03-21
  21. Comprehensive metabolomics expands precision medicine for triple-negative breast cancer
    Authors: Y Xiao, D Ma, YS Yang, F Yang, JH Ding, Y Gong, L Jiang, LP Ge, SY Wu, Q Yu, Q Zhang, F Bertucci, Q Sun, X Hu, DQ Li, ZM Shao, YZ Jiang
    Cell Research, 2022-02-01;0(0):.  2022-02-01
  22. An in�vitro chronic damage model impairs inflammatory and regenerative responses in human colonoid monolayers
    Authors: WD Rees, N Telkar, DTS Lin, MQ Wong, C Poloni, A Fathi, M Kobor, NC Zachos, TS Steiner
    Cell Reports, 2022-01-18;38(3):110283.  2022-01-18
  23. CHRONIC INFLAMMATION IN ULCERATIVE COLITIS CAUSES LONG TERM CHANGES IN GOBLET CELL FUNCTION
    Authors: S Varsha, J Kelli, Y Jianyi, L Sun, L Ruxian, Y Huimin, I Julie, FA Jennifer, ZC Nicholas, D Mark, R Yan
    Cellular and Molecular Gastroenterology and Hepatology, 2021-08-18;0(0):.  2021-08-18
  24. Tumor Extracellular Vesicles Regulate Macrophage-Driven Metastasis through CCL5
    Authors: DC Rabe, ND Walker, FD Rustandy, J Wallace, J Lee, SL Stott, MR Rosner
    Cancers, 2021-07-10;13(14):.  2021-07-10
  25. Sonlicromanol's active metabolite KH176m normalizes prostate cancer stem cell mPGES-1 overexpression and inhibits cancer spheroid growth
    Authors: X Jiang, H Renkema, J Smeitink, J Beyrath
    PLoS ONE, 2021-07-09;16(7):e0254315.  2021-07-09
  26. Successful Patient-Derived Organoid Culture of Gynecologic Cancers for Disease Modeling and Drug Sensitivity Testing
    Authors: J Bi, AM Newtson, Y Zhang, EJ Devor, MI Samuelson, KW Thiel, KK Leslie
    Cancers, 2021-06-10;13(12):.  2021-06-10
  27. In Situ Vitrification of Lung Cancer Organoids on a Microwell Array
    Authors: Q Liu, T Zhao, X Wang, Z Chen, Y Hu, X Chen
    Micromachines, 2021-05-28;12(6):.  2021-05-28
  28. Lung cancer organoids analyzed on microwell arrays predict drug responses of patients within a week
    Authors: Y Hu, X Sui, F Song, Y Li, K Li, Z Chen, F Yang, X Chen, Y Zhang, X Wang, Q Liu, C Li, B Zou, X Chen, J Wang, P Liu
    Nature Communications, 2021-05-10;12(1):2581.  2021-05-10
  29. The Fragile X Mental Retardation Protein regulates RIP1K and colorectal cancer resistance to necroptosis
    Authors: A Di Grazia, I Marafini, G Pedini, D Di Fusco, F Laudisi, V Dinallo, E Rosina, C Stolfi, E Franzè, P Sileri, G Sica, G Monteleone, C Bagni, I Monteleone
    Cell Mol Gastroenterol Hepatol, 2020-10-19;0(0):.  2020-10-19
  30. A Human Organoid Model of Aggressive Hepatoblastoma for Disease Modeling and Drug Testing
    Authors: JA Saltsman, WJ Hammond, NJC Narayan, D Requena, H Gehart, G Lalazar, MP LaQuaglia, H Clevers, S Simon
    Cancers, 2020-09-18;12(9):.  2020-09-18
  31. Human BCL-G regulates secretion of inflammatory chemokines but is dispensable for induction of apoptosis by IFN-gamma and TNF-alpha in intestinal epithelial cells
    Authors: JA Woznicki, P Flood, M Bustamante, P Stamou, G Moloney, A Fanning, SA Zulquernai, J McCarthy, F Shanahan, S Melgar, K Nally
    Cell Death Dis, 2020-01-27;11(1):68.  2020-01-27
  32. Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids
    Authors: B Artegiani, L van Voorth, RGH Lindeboom, D Seinstra, I Heo, P Tapia, C López-Igle, D Postrach, T Dayton, R Oka, H Hu, R van Boxtel, JH van Es, J Offerhaus, PJ Peters, J van Rheene, M Vermeulen, H Clevers
    Cell Stem Cell, 2019-05-23;0(0):.  2019-05-23
  33. Renal expression of JAK2 is high in polycystic kidney disease and its inhibition reduces cystogenesis
    Authors: F Patera, A Cudzich-Ma, Z Huang, M Fragiadaki
    Sci Rep, 2019-03-14;9(1):4491.  2019-03-14

FAQs

  1. Can the Organoid Harvesting Solution, Catalog # 3700-100-01,  be used to dissociate cells grown on a substrate of Cultrex Rat Collagen 1?

    • The Organoid Harvesting Solution works well with BME (Basement Membrane Extract).  For cells grown on Cultrex Rat Collagen 1, the recommended enzyme to use to dissociate cells is Collagenase. Collagenase treatment at 37oC for 30 minutes is recommended.

  2. Are Cultrex Organoid Harvesting Solution (Catalog # 3700-100-01) and dispase interchangeable?

    • Cultrex Organoid Harvesting Solution (Catalog # 3700-100-01) is a non-enzymatic solution that depolymerizes the matrix to enable harvest of organoids. In contrast, dispase is a protease enzyme that degrades the matrix. End users will need to empirically determine whether enzymatic or non-enzymatic solutions are more appropriate for their experimental setup.

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Reviews for Cultrex Organoid Harvesting Solution

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Cultrex Organoid Harvesting Solution
By MARIA PINNA on 05/17/2023

I have been using Organoid Harvesting Solutions since 2018 for organoid models biochemical analysis.
It's very easy to use and allows you to dissociate and depolymerize any basement membrane matrix (Ultimatrix, BME Type 2 Selec, Matrigel) to harvest intact organoids.