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Cultrex Organoid Harvesting Solution

3700-100-01 3 Citations Datasheet / COA / SDS
Ready-to-use, non-enzymatic organoid harvesting and dissociation solution
Catalog # Availability Size / Price Qty
3700-100-01
Product Details
Citations (3)
FAQs
Reviews
Summary Product Datasheets Background

Cultrex Organoid Harvesting Solution Summary

Ready-to-use, non-enzymatic solution to depolymerize basement membrane matrix for harvesting organoids from culture.

Why Use Cultrex Organoid Harvesting Solution?

Organoid cultures exhibit cellular behaviors and morphologies similar to those seen in vivo, however, the adaptation of these models for studying biochemical processes has been impeded by the challenge of separating intact organoids from extra-cellular proteins comprising the hydrogel. Commonly, proteases are employed to degrade these extracellular proteins, however, proteases also degrade proteins on the cell surface and protease activity may carry over into subsequent cultures or lysate preparations. Cultrex Organoid Harvesting Solution provides a non-enzymatic method for depolymerizing extracellular matrix proteins to allow for harvesting of intact organoids for passaging, cryopreservation, or biochemical analysis.

Specifications

Stability
Product is stable for at least 2 months from the date of receipt when stored at 2-8 °C. Keep sterile. See lot specific Certificate of Analysis for expiration.
Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at 2 - 8 °C.
Species
N/A

Limitations

For research use only. Not for diagnostic use.

Product Datasheets

Product Datasheet
COA
SDS

Citations for Cultrex Organoid Harvesting Solution

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. A Human Organoid Model of Aggressive Hepatoblastoma for Disease Modeling and Drug Testing
    Authors: JA Saltsman, WJ Hammond, NJC Narayan, D Requena, H Gehart, G Lalazar, MP LaQuaglia, H Clevers, S Simon
    Cancers, 2020;12(9):.  2020
  2. Renal expression of JAK2 is high in polycystic kidney disease and its inhibition reduces cystogenesis
    Authors: F Patera, A Cudzich-Ma, Z Huang, M Fragiadaki
    Sci Rep, 2019;9(1):4491.  2019
  3. Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids
    Authors: B Artegiani, L van Voorth, RGH Lindeboom, D Seinstra, I Heo, P Tapia, C López-Igle, D Postrach, T Dayton, R Oka, H Hu, R van Boxtel, JH van Es, J Offerhaus, PJ Peters, J van Rheene, M Vermeulen, H Clevers
    Cell Stem Cell, 2019;0(0):.  2019

FAQs

  1. Can the Organoid Harvesting Solution, Catalog # 3700-100-01,  be used to dissociate cells grown on a substrate of Cultrex Rat Collagen 1?

    • The Organoid Harvesting Solution works well with BME (Basement Membrane Extract).  For cells grown on Cultrex Rat Collagen 1, the recommended enzyme to use to dissociate cells is Collagenase. Collagenase treatment at 37oC for 30 minutes is recommended.

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