Cultrex Organoid Harvesting Solution
Cultrex Organoid Harvesting Solution Summary
Why Use Cultrex Organoid Harvesting Solution?
Organoid cultures exhibit cellular behaviors and morphologies similar to those seen in vivo, however, the adaptation of these models for studying biochemical processes has been impeded by the challenge of separating intact organoids from extra-cellular proteins comprising the hydrogel. Commonly, proteases are employed to degrade these extracellular proteins, however, proteases also degrade proteins on the cell surface and protease activity may carry over into subsequent cultures or lysate preparations. Cultrex Organoid Harvesting Solution provides a non-enzymatic method for depolymerizing extracellular matrix proteins to allow for harvesting of intact organoids for passaging, cryopreservation, or biochemical analysis.
For research use only. Not for diagnostic use.
Citations for Cultrex Organoid Harvesting Solution
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Citations: Showing 1 - 3
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A Human Organoid Model of Aggressive Hepatoblastoma for Disease Modeling and Drug Testing
Authors: JA Saltsman, WJ Hammond, NJC Narayan, D Requena, H Gehart, G Lalazar, MP LaQuaglia, H Clevers, S Simon
Cancers, 2020;12(9):. 2020
Renal expression of JAK2 is high in polycystic kidney disease and its inhibition reduces cystogenesis
Authors: F Patera, A Cudzich-Ma, Z Huang, M Fragiadaki
Sci Rep, 2019;9(1):4491. 2019
Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids
Authors: B Artegiani, L van Voorth, RGH Lindeboom, D Seinstra, I Heo, P Tapia, C López-Igle, D Postrach, T Dayton, R Oka, H Hu, R van Boxtel, JH van Es, J Offerhaus, PJ Peters, J van Rheene, M Vermeulen, H Clevers
Cell Stem Cell, 2019;0(0):. 2019
Can the Organoid Harvesting Solution, Catalog # 3700-100-01, be used to dissociate cells grown on a substrate of Cultrex Rat Collagen 1?
The Organoid Harvesting Solution works well with BME (Basement Membrane Extract). For cells grown on Cultrex Rat Collagen 1, the recommended enzyme to use to dissociate cells is Collagenase. Collagenase treatment at 37oC for 30 minutes is recommended.
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