Cultrex PathClear 3-D Culture Matrix RGF BME
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Cultrex PathClear 3-D Culture Matrix RGF BME Summary
- Tube formation assay - BME promotes formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells.
- 3D Culture - Basement Membrane Extract promotes differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) or human prostate (PC-3) into acinar structures.
Gelling assay: BME gels in less than 30 minutes at 37°C, and maintains the gelled form in culture medium for a minimum of 14 days at 37°C.
Murine Engelbreth-Holm-Swarm (EHS) tumor.
|Formulation||14 - 16 mg/mL.
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Product is stable for a minimum of 3 months from date of shipment when stored at –20°C in a manual defrost freezer. For optimal stability, store at –80°C. Avoid freeze-thaw cycles.
Storage buffer: Dulbecco’s Modified Eagle’s medium without phenol red, with 10 μg/ml gentamicin sulfate.
• PathClear® - Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses.
• No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP sterility testing guidelines.
Background: Basement Membrane Extracts
3-D Culture is a cell culture method that provides cells with the
necessary structure and signaling cues to direct reconstruction of the
tissue architecture, and these methods provide physiologically
predictive in vitro models for evaluating development and disease.
Normal cells assemble into organiods that structurally resemble their
emanating tissues, exhibit a polarized morphology, undergo cell cycle
regulation, and produce tissuespecific proteins. While cancer cells
assemble into tumor-like structures, lacking an organized architecture
or cell cycle regulation and exhibiting tumor-specific markers depending
on the extend of malignancy.
To aid in the advancement of this technology, Trevigen has developed Cultrex® 3-D Culture Matrix™ RGF BME which is the first Basement Membrane Extract produced and qualified specifically for use in 3-D culture studies. The 3-D Culture Matrix™ RGF BME provides the foundation for cells to grow in three dimensions allowing for the formation of structures in vitro. To provide the most standardized basement membrane extract for use in 3-D cultures, a special process is employed to reduce growth factors and manufacture matrix at a standard concentration of approximately 15 mg/ml. This product is then evaluated in a 3D culture to validate efficacy.
How does Cultrex® BME promote cell differentiation?
All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic substrates, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.
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