<8.0 EU per 1 μg of the protein by the LAL method.
Functional Assay: Tube formation - BME promotes formation
of capillary-like structures by human (HBMVEC; HUVEC) or mouse
(SVEC4-10) endothelial cells. Gelling Assay: BME gels in less than 30 minutes at 37°C, and maintains the gelled form in culture medium for a minimum of 14 days at 37°C.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Product is stable for a minimum of 3 months from date of shipment when
stored at –20°C in a manual defrost freezer. For optimal stability,
store at –80°C. Avoid freeze-thaw cycles. Storage Buffer: Dulbecco’s Modified Eagle’s medium without phenol red, with 10 ug/ml gentamicin sulfate.
Stability Testing: •
PathClear® - Negative by PCR test for mycoplasma; 17 bacterial and
virus strains typically included in mouse antibody production (MAP)
testing, plus 13 additional murine infectious agents including LDEV, for
a total of 31 organisms and viruses. • No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP sterility testing guidelines.
Background: Basement Membrane Extracts
Basement membranes are continuous sheets of specialized extracellular
matrix that form an interface between endothelial, epithelial, muscle,
or neuronal cells and their adjacent stroma. Basement membranes are
degraded and regenerated during development and wound healing. They not
only support cells and cell layers, but they also play an essential role
in tissue organization that affects cell adhesion, migration,
proliferation, and differentiation. Basement membranes provide major
barriers to invasion by metastatic tumor cells. Cultrex® Basement
Membrane Extract (BME) is a soluble form of basement membrane purified
from Engelbreth-Holm-Swarm (EHS) tumor. The extract gels at 37°C to form
a reconstituted basement membrane. The major components of BME include
laminin, collagen IV, entactin, and heparin sulfate proteoglycan. BME
can be used in a multiple applications, under a variety of cell culture
conditions, for maintaining growth or promoting differentiation of
primary endothelial, epithelial, smooth muscle and stem cells. BME can
also be utilized in cell attachment, neurite outgrowth, angiogenesis, in vitro cell invasion and in vivo tumorigenicity assays.
Basement Membrane Extracts
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
How does Cultrex® BME promote cell differentiation?
All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic substrates, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.
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