Cultrex PathClear Basement Membrane Extract

Newer Version Available: 3432-005-02
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Product Details
Citations (34)
Reviews (1)

Cultrex PathClear Basement Membrane Extract Summary

Endotoxin Level
<8.0 EU per 1 μg of the protein by the LAL method.
Functional Assay: Tube formation - BME promotes formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells.

Gelling Assay:
BME gels in less than 30 minutes at 37°C, and maintains the gelled form in culture medium for a minimum of 14 days at 37°C.

Murine Engelbreth-Holm-Swarm (EHS) tumor.

Product Datasheets


Formulation 12 - 18 mg/mL.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Product is stable for a minimum of 3 months from date of shipment when stored at -20 °C in a manual defrost freezer. For optimal stability, store at -80 °C. Avoid freeze-thaw cycles.

Storage Buffer: Dulbecco’s Modified Eagle’s medium without phenol red, with 10 ug/ml gentamicin sulfate.

Sterility Testing: PathClear® - Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses. No bacterial or fungal growth detected after incubation at 37 °C for 14 days following USP sterility testing guidelines.
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Background: Basement Membrane Extracts

Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma. Basement membranes are degraded and regenerated during development and wound healing. They not only support cells and cell layers, but they also play an essential role in tissue organization that affects cell adhesion, migration, proliferation, and differentiation. Basement membranes provide major barriers to invasion by metastatic tumor cells.  Cultrex® Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. The extract gels at 37°C to form a reconstituted basement membrane. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycan.  BME can be used in a multiple applications, under a variety of cell culture conditions, for maintaining growth or promoting differentiation of primary endothelial, epithelial, smooth muscle and stem cells. BME can also be utilized in cell attachment, neurite outgrowth, angiogenesis, in vitro cell invasion and in vivo tumorigenicity assays.

Alternate Names
Basement Membrane Extracts

Citations for Cultrex PathClear Basement Membrane Extract

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

34 Citations: Showing 1 - 10
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  1. Enteroendocrine cells switch hormone expression along the crypt-to-villus BMP signalling gradient
    Authors: J Beumer, B Artegiani, Y Post, F Reimann, F Gribble, TN Nguyen, H Zeng, M Van den Bo, JH Van Es, H Clevers
    Nat. Cell Biol., 2018;20(8):909-916.
    Species: Human
    Sample Types: organoid
    Applications: Bioassay
  2. Transposon mutagenesis identifies genes that cooperate with mutant Pten in breast cancer progression
    Proc. Natl. Acad. Sci. U.S.A., 2016;0(0):.
  3. Wnt signaling promotes breast cancer by blocking ITCH-mediated degradation of the YAP/TAZ transcriptional coactivator WBP2
    Cancer Res, 2016;0(0):.
    Species: Mouse
    Sample Types: In Vivo
  4. Cabozantinib (XL184) Inhibits Growth and Invasion of Preclinical TNBC Models.
    Authors: Sameni M, Tovar E, Essenburg C, Chalasani A, Linklater E, Borgman A, Cherba D, Anbalagan A, Winn M, Graveel C, Sloane B
    Clin Cancer Res, 2015;22(4):923-34.
  5. Simian immunodeficiency virus and human immunodeficiency virus type 1 matrix proteins specify different capabilities to modulate B cell growth.
    Authors: Caccuri, Francesc, Giagulli, Cinzia, Reichelt, Joachim, Martorelli, Debora, Marsico, Stefania, Bugatti, Antonell, Barone, Ines, Rusnati, Marco, Guzman, Carlos A, Dolcetti, Riccardo, Caruso, Arnaldo
    J Virol, 2014;88(10):5706-17.
  6. Inhibition of endothelial progenitor cell differentiation by VEGI.
    Authors: Tian F, Liang PH, Li LY
    Blood, 2009;113(21):5352-60.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Direct interaction of focal adhesion kinase (FAK) with Met is required for FAK to promote hepatocyte growth factor-induced cell invasion.
    Authors: Chen SY, Chen HC
    Mol. Cell. Biol., 2006;26(13):5155-67.
    Species: Canine
    Sample Types: Whole Cells
  8. The nuclear receptor peroxisome proliferator-activated receptor-beta/delta (PPARbeta/delta) promotes oncogene-induced cellular senescence through repression of endoplasmic reticulum stress.
    Authors: Zhu B, Ferry C, Markell L, Blazanin N, Glick A, Gonzalez F, Peters J
    J Biol Chem, 0;289(29):20102-19.
  9. Inhibition of tumor growth and angiogenesis by SP-2, an anti-lectin, galactoside-binding soluble 3 binding protein (LGALS3BP) antibody.
    Authors: Traini S, Piccolo E, Tinari N, Rossi C, La Sorda R, Spinella F, Bagnato A, Lattanzio R, D'Egidio M, Di Risio A, Tomao F, Grassadonia A, Piantelli M, Natoli C, Iacobelli S
    Mol Cancer Ther, 0;13(4):916-25.
  10. The interplay between hypoxia, endothelial and melanoma cells regulates vascularization and cell motility through endothelin-1 and vascular endothelial growth factor.
    Authors: Spinella F, Caprara V, Cianfrocca R, Rosano L, Di Castro V, Garrafa E, Natali P, Bagnato A
    Carcinogenesis, 0;35(4):840-8.
  11. The MEF2-HDAC axis controls proliferation of mammary epithelial cells and acini formation in vitro.
    Authors: Clocchiatti A, Di Giorgio E, Viviani G, Streuli C, Sgorbissa A, Picco R, Cutano V, Brancolini C
    J Cell Sci, 0;128(21):3961-76.
  12. Mouse embryonic dorsal root ganglia contain pluripotent stem cells that show features similar to embryonic stem cells and induced pluripotent stem cells.
    Authors: Ogawa R, Fujita K, Ito K
    Biol Open, 0;6(5):602-618.
  13. Microfluidics separation reveals the stem-cell-like deformability of tumor-initiating cells.
    Authors: Zhang W, Kai K, Choi D, Iwamoto T, Nguyen Y, Wong H, Landis M, Ueno N, Chang J, Qin L
    Proc Natl Acad Sci U S A, 0;109(46):18707-12.
  14. Translation of a tumor microenvironment mimicking 3D tumor growth co-culture assay platform to high-content screening.
    Authors: Krausz E, de Hoogt R, Gustin E, Cornelissen F, Grand-Perret T, Janssen L, Vloemans N, Wuyts D, Frans S, Axel A, Peeters P, Hall B, Cik M
    J Biomol Screen, 0;18(1):54-66.
  15. Suppression of ERbeta signaling via ERbeta knockout or antagonist protects against bladder cancer development.
    Authors: Hsu I, Chuang K, Slavin S, Da J, Lim W, Pang S, O'Brien J, Yeh S
    Carcinogenesis, 0;35(3):651-61.
  16. Markers of oxidative stress in umbilical cord blood from G6PD deficient African newborns.
    Authors: Stadem P, Hilgers M, Bengo D, Cusick S, Ndidde S, Slusher T, Lund T
    PLoS ONE, 0;12(2):e0172980.
  17. Real-time monitoring of NKCC2 endocytosis by total internal reflection fluorescence (TIRF) microscopy.
    Authors: Jaykumar A, Caceres P, Sablaban I, Tannous B, Ortiz P
    Am J Physiol Renal Physiol, 0;310(2):F183-91.
  18. Preclinical Antitumor Efficacy of BAY 1129980-a Novel Auristatin-Based Anti-C4.4A (LYPD3) Antibody-Drug Conjugate for the Treatment of Non-Small Cell Lung Cancer.
    Authors: Willuda J, Linden L, Lerchen H, Kopitz C, Stelte-Ludwig B, Pena C, Lange C, Golfier S, Kneip C, Carrigan P, McLean K, Schuhmacher J, von Ahsen O, Muller J, Dittmer F, Beier R, El Sheikh S, Tebbe J, Leder G, Apeler H, Jautelat R, Ziegelbauer K, Kreft B
    Mol Cancer Ther, 0;16(5):893-904.
  19. MELK-T1, a small-molecule inhibitor of protein kinase MELK, decreases DNA-damage tolerance in proliferating cancer cells.
    Authors: Beke L, Kig C, Linders J, Boens S, Boeckx A, van Heerde E, Parade M, De Bondt A, Van den Wyngaert I, Bashir T, Ogata S, Meerpoel L, Van Eynde A, Johnson C, Beullens M, Brehmer D, Bollen M
    Biosci Rep, 0;35(6):.
  20. Timing and targeting: the biological functions of Dam methylation in E. coli.
    Authors: Messer W, Noyer-Weidner M
    Cell, 0;54(6):735-7.
  21. Involvement of the heparanase procoagulant domain in bleeding and wound healing.
    Authors: Crispel Y, Ghanem S, Attias J, Kogan I, Brenner B, Nadir Y
    J Thromb Haemost, 0;15(7):1463-1472.
  22. Combined Effects of Suberoylanilide Hydroxamic Acid and Cisplatin on Radiation Sensitivity and Cancer Cell Invasion in Non-Small Cell Lung Cancer.
    Authors: Feng J, Zhang S, Wu K, Wang B, Wong J, Jiang H, Xu R, Ying L, Huang H, Zheng X, Chen X, Ma S
    Mol Cancer Ther, 0;15(5):842-53.
  23. Dickkopf-3 regulates prostate epithelial cell acinar morphogenesis and prostate cancer cell invasion by limiting TGF-beta-dependent activation of matrix metalloproteases.
    Authors: Romero D, Al-Shareef Z, Gorrono-Etxebarria I, Atkins S, Turrell F, Chhetri J, Bengoa-Vergniory N, Zenzmaier C, Berger P, Waxman J, Kypta R
    Carcinogenesis, 0;37(1):18-29.
  24. Wnt5a Drives an Invasive Phenotype in Human Glioblastoma Stem-like Cells.
    Authors: Binda E, Visioli A, Giani F, Trivieri N, Palumbo O, Restelli S, Dezi F, Mazza T, Fusilli C, Legnani F, Carella M, Di Meco F, Duggal R, Vescovi A
    Cancer Res, 0;77(4):996-1007.
  25. A humanized single-chain antibody against beta 3 integrin inhibits pulmonary metastasis by preferentially fragmenting activated platelets in the tumor microenvironment.
    Authors: Zhang W, Dang S, Hong T, Tang J, Fan J, Bu D, Sun Y, Wang Z, Wisniewski T
    Blood, 0;120(14):2889-98.
  26. PI3K/mTOR dual inhibitor VS-5584 preferentially targets cancer stem cells.
    Authors: Kolev V, Wright Q, Vidal C, Ring J, Shapiro I, Ricono J, Weaver D, Padval M, Pachter J, Xu Q
    Cancer Res, 0;75(2):446-55.
  27. HIV-1 matrix protein p17 promotes angiogenesis via chemokine receptors CXCR1 and CXCR2.
    Authors: Caccuri F, Giagulli C, Bugatti A, Benetti A, Alessandri G, Ribatti D, Marsico S, Apostoli P, Slevin M, Rusnati M, Guzman C, Fiorentini S, Caruso A
    Proc Natl Acad Sci U S A, 0;109(36):14580-5.
  28. A human papillomavirus (HPV) in vitro neutralization assay that recapitulates the in vitro process of infection provides a sensitive measure of HPV L2 infection-inhibiting antibodies.
    Authors: Day P, Pang Y, Kines R, Thompson C, Lowy D, Schiller J
    Clin Vaccine Immunol, 0;19(7):1075-82.
  29. Regulation of P21-activated kinase-4 by progesterone and tumor necrosis factor-alpha in human endometrium and its increased expression in advanced-stage endometriosis.
    Authors: Kim S, Kim S, Ihm H, Oh Y, Chae H, Kim C, Kang B
    J Clin Endocrinol Metab, 0;98(2):E238-48.
  30. Dual Targeting of Mesenchymal and Amoeboid Motility Hinders Metastatic Behavior.
    Authors: Jones B, Kelley L, Loskutov Y, Marinak K, Kozyreva V, Smolkin M, Pugacheva E
    Mol Cancer Res, 0;15(6):670-682.
  31. Cathepsin B inhibition limits bone metastasis in breast cancer.
    Authors: Withana N, Blum G, Sameni M, Slaney C, Anbalagan A, Olive M, Bidwell B, Edgington L, Wang L, Moin K, Sloane B, Anderson R, Bogyo M, Parker B
    Cancer Res, 0;72(5):1199-209.
  32. Anti-invasive adjuvant therapy with imipramine blue enhances chemotherapeutic efficacy against glioma.
    Authors: Munson J, Fried L, Rowson S, Bonner M, Karumbaiah L, Diaz B, Courtneidge S, Knaus U, Brat D, Arbiser J, Bellamkonda R
    Sci Transl Med, 0;4(127):127ra36.
  33. Suppression of the epithelial-mesenchymal transition by Grainyhead-like-2.
    Authors: Cieply B, Riley P, Pifer P, Widmeyer J, Addison J, Ivanov A, Denvir J, Frisch S
    Cancer Res, 0;72(9):2440-53.
  34. p16 controls epithelial cell growth and suppresses carcinogenesis through mechanisms that do not require RB1 function.
    Authors: Sen M, Akeno N, Reece A, Miller A, Simpson D, Wikenheiser-Brokamp K
    Oncogenesis, 0;6(4):e320.


  1. How does Cultrex® BME promote cell differentiation?

    • All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic substrates, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.

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Cultrex PathClear Basement Membrane Extract
By Anonymous on 04/01/2018
Application: Cell migration/motility