Cultrex Rat Collagen I

Purified stromal extracellular matrix protein that can promte cell adhesion for cell and tissue cultures
Catalog # Availability Size / Price Qty
3440-005-01
3440-100-01
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Cultrex Rat Collagen I Summary

Cultrex Rat Collagen I is a purified stromal extracellular matrix protein that can promtes cell adhesion for cell and tissue culture. It may also be used to supplement customized coatings, hydrogels, bioinks, or medium formulations for cell culture.

Key Benefits

• Qualified for general cell culture applications
• May be used as a hydrogel or thin coating for tissue culture vessels
• Material provided at a standard concentration of ~5 mg/mL

Why Use Cultrex Rat Collagen I?

Cultrex Rat Collagen I is purified from rat tail tendons and is provided at a high concentration that is capable of polymerizing to form a hydrogel. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37̊ C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I, a special process is employed to provide material at a standard concentration of approximately 5 mg/mL.

Specifications

Source
Rat tail tendons
Protein Concentration
5 mg/mL
Endotoxin Level
≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Testing Cell Culture
Supports cell attachment and spreading of HT1080 human fibrosarcoma cells.
Viral Testing
No mycoplasma contamination detected by PCR.
Stability
Product is stable for at least 6 months from the date of receipt when stored at 2-8 °C. Keep sterile.
Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at 2 - 8 °C.
Species
Rat

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Reagents Provided

Other Reagents Provided

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