Cultrex Rat Collagen I

Purified stromal extracellular matrix protein that promotes cell adhesion for cell and tissue cultures
Catalog # Availability Size / Price Qty
3440-005-01
3440-100-01
Product Details
Citations (31)
FAQs
Reviews

Cultrex Rat Collagen I Summary

Cultrex Rat Collagen I is a purified stromal extracellular matrix protein that promotes cell adhesion for cell and tissue culture. It may also be used to supplement customized coatings, hydrogels, bioinks, or medium formulations for cell culture.

Key Benefits

• Qualified for general cell culture applications
• May be used as a hydrogel or thin coating for tissue culture vessels
• Material provided at a standard concentration of ~5 mg/mL

Why Use Cultrex Rat Collagen I?

Cultrex Rat Collagen I is purified from rat tail tendons and is provided at a high concentration that is capable of polymerizing to form a hydrogel. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37̊ C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I, a special process is employed to provide material at a standard concentration of approximately 5 mg/mL.

Specifications

Source
Rat tail tendons
Protein Concentration
5 mg/mL
Endotoxin Level
≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Testing Cell Culture
Supports cell attachment and spreading of HT1080 human fibrosarcoma cells.
Viral Testing
No mycoplasma contamination detected by PCR.
Stability
Product is stable for a minimum of 2 months if stored at 2-8 °C. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at 2 - 8 °C.
Species
Rat

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Citations for Cultrex Rat Collagen I

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. The ER Unfolded Protein Response Effector, ATF6, Reduces Cardiac Fibrosis and Decreases Activation of Cardiac Fibroblasts
    Authors: WT Stauffer, EA Blackwood, K Azizi, RJ Kaufman, CC Glembotski
    Int J Mol Sci, 2020;21(4):.  2020
  2. Circular RNA USP1 regulates the permeability of blood-tumour barrier via miR-194-5p/FLI1 axis
    Authors: Y Gao, P Wu, Y Ma, Y Xue, Y Liu, J Zheng, X Liu, Q He, J Ma, L Liu, P Wang
    J. Cell. Mol. Med., 2019;0(0):.  2019
  3. Interaction of Treponema pallidum, the syphilis spirochete, with human platelets
    Authors: B Church, E Wall, JR Webb, CE Cameron
    PLoS ONE, 2019;14(1):e0210902.  2019
  4. Randomly Distributed K14+ Breast Tumor Cells Polarize to the Leading Edge and Guide Collective Migration in Response to Chemical and Mechanical Environmental Cues
    Authors: PY Hwang, A Brenot, AC King, GD Longmore, SC George
    Cancer Res., 2019;79(8):1899-1912.  2019
  5. Formation of three-dimensional tubular endothelial cell networks under defined serum-free cell culture conditions in human collagen hydrogels
    Authors: B Andrée, H Ichanti, S Kalies, A Heisterkam, S Strau beta, PM Vogt, A Haverich, A Hilfiker
    Sci Rep, 2019;9(1):5437.  2019
  6. Tumour-treating fields (TTFields): Investigations on the mechanism of action by electromagnetic exposure of cells in telophase/cytokinesis
    Authors: L Berkelmann, A Bader, S Meshksar, A Dierks, G Hatipoglu, JK Krauss, K Schwabe, D Manteuffel, A Ngezahayo
    Sci Rep, 2019;9(1):7362.  2019
  7. Zika virus enhances monocyte adhesion and transmigration favoring viral dissemination to neural cells
    Authors: NV Ayala-Nune, G Follain, F Delalande, A Hirschler, E Partiot, GL Hale, BC Bollweg, J Roels, M Chazal, F Bakoa, M Carocci, S Bourdoulou, O Faklaris, SR Zaki, A Eckly, B Uring-Lamb, F Doussau, S Cianferani, C Carapito, FMJ Jacobs, N Jouvenet, JG Goetz, R Gaudin
    Nat Commun, 2019;10(1):4430.  2019
  8. Biosynthetic CircRNA_001160 induced by PTBP1 regulates the permeability of BTB via the CircRNA_001160/miR-195-5p/ETV1 axis
    Authors: H Li, S Shen, X Ruan, X Liu, J Zheng, Y Liu, C Yang, D Wang, L Liu, J Ma, T Ma, P Wang, H Cai, Z Li, L Zhao, Y Xue
    Cell Death Dis, 2019;10(12):960.  2019
  9. Development of a Gut-On-A-Chip Model for High Throughput Disease Modeling and Drug Discovery
    Authors: C Beaurivage, E Naumovska, YX Chang, ED Elstak, A Nicolas, H Wouters, G van Moolen, HL Lanz, SJ Trietsch, J Joore, P Vulto, RAJ Janssen, KS Erdmann, J Stallen, D Kurek
    Int J Mol Sci, 2019;20(22):.  2019
  10. Peptide hormone ELABELA enhances extravillous trophoblast differentiation, but placenta is not the major source of circulating ELABELA in pregnancy
    Authors: D Georgiadou, S Boussata, WHM Ranzijn, LEA Root, S Hillenius, JM Bij de Weg, CNH Abheiden, MA de Boer, JIP de Vries, TGM Vrijkotte, CB Lambalk, EAM Kuijper, GB Afink, M van Dijk
    Sci Rep, 2019;9(1):19077.  2019
  11. Smooth muscle cells differentiated from human mesenchymal stem cells regulated by microRNA (miR)-503 and miR-222-5p are suitable for vascular tissue engineering
    Authors: W Gu, X Hong, A Le Bras, WN Nowak, S Issa Bhalo, J Deng, Y Xie, Y Hu, XZ Ruan, Q Xu
    J. Biol. Chem., 2018;0(0):.  2018
  12. Increased macrophages and�changed brain endothelial cell gene expression in the frontal cortex of people with schizophrenia displaying inflammation
    Authors: HQ Cai, VS Catts, MJ Webster, C Galletly, D Liu, M O'Donnell, TW Weickert, CS Weickert
    Mol. Psychiatry, 2018;0(0):.  2018
  13. Protein S and Gas6 induce efferocytosis of HIV-1-infected cells
    Authors: BA Chua, JA Ngo, K Situ, CM Ramirez, H Nakano, K Morizono
    Virology, 2018;515(0):176-190.  2018
  14. Mechanism of piR-DQ590027/MIR17HG regulating the permeability of glioma conditioned normal BBB
    Authors: X Leng, J Ma, Y Liu, S Shen, H Yu, J Zheng, X Liu, L Liu, J Chen, L Zhao, X Ruan, Y Xue
    J. Exp. Clin. Cancer Res., 2018;37(1):246.  2018
  15. Trojan Horse Transit Contributes to Blood-Brain Barrier Crossing of a Eukaryotic Pathogen
    Authors: FH Santiago-T, MD Onken, JA Cooper, RS Klein, TL Doering
    MBio, 2017;8(1):.  2017
  16. Knockdown of long non-coding RNA XIST increases blood-tumor barrier permeability and inhibits glioma angiogenesis by targeting miR-137
    Authors: H Yu, Y Xue, P Wang, X Liu, J Ma, J Zheng, Z Li, Z Li, H Cai, Y Liu
    Oncogenesis, 2017;6(3):e303.  2017
  17. Enhanced transport of plant-produced rabies single chain antibody-RVG peptide fusion protein across an in cellulo blood-brain barrier device
    Authors: W Phoolcharo, C Prehaud, CJ van Dollew, L Both, A da Costa, M Lafon, JK Ma
    Plant Biotechnol. J, 2017;0(0):.  2017
  18. PVT1 affects growth of glioma microvascular endothelial cells by negatively regulating miR-186
    Authors: Y Ma, P Wang, Y Xue, C Qu, J Zheng, X Liu, J Ma, Y Liu
    Tumour Biol., 2017;39(3):1010428317694.  2017
  19. Multifunctional LUV liposomes decorated for BBB and amyloid targeting. A. In vitro proof-of-concept
    Authors: K Papadia, E Markoutsa, S Mourtas, AD Giannou, B La Ferla, F Nicotra, M Salmona, P Klepetsani, GT Stathopoul, SG Antimisiar
    Eur J Pharm Sci, 2017;101(0):140-148.  2017
  20. MiR-18a increased the permeability of BTB via RUNX1 mediated down-regulation of ZO-1, occludin and claudin-5.
    Authors: Miao Y, Zhao Y, Zhao L, Wang P, Liu Y, Ma J, Xue Y
    Cell Signal, 2015;27(1):156-67.  2015
  21. Maspin Expression in Prostate Tumor Cells Averts Stemness and Stratifies Drug Sensitivity.
    Authors: Bernardo M, Kaplun A, Dzinic S, Li X, Irish J, Mujagic A, Jakupovic B, Back J, Van Buren E, Han X, Dean I, Chen Y, Heath E, Sakr W, Sheng S
    Cancer Res, 2015;75(18):3970-9.  2015
  22. DOCK8 regulates lymphocyte shape integrity for skin antiviral immunity.
    Authors: Zhang, Qian, Dove, Christop, Hor, Jyh Lian, Murdock, Heardley, Strauss-Albee, Dara M, Garcia, Jordan A, Mandl, Judith N, Grodick, Rachael, Jing, Huie, Chandler-Brown, Devon B, Lenardo, Timothy, Crawford, Greg, Matthews, Helen F, Freeman, Alexandr, Cornall, Richard, Germain, Ronald N, Mueller, Scott N, Su, Helen C
    J Exp Med, 2014;211(13):2549-66.  2014
  23. Jab1 regulates Schwann cell proliferation and axonal sorting through p27.
    Authors: Porrello, Emanuela, Rivellini, Cristina, Dina, Giorgia, Triolo, Daniela, Del Carro, Ubaldo, Ungaro, Daniela, Panattoni, Martina, Feltri, Maria La, Wrabetz, Lawrence, Pardi, Ruggero, Quattrini, Angelo, Previtali, Stefano
    J Exp Med, 2014;211(1):29-43.  2014
  24. Transcellular targeting of fiber- and hexon-modified adenovirus vectors across the brain microvascular endothelial cells in vitro.
    Authors: Laakkonen, Johanna, Engler, Tatjana, Romero, Ignacio, Weksler, Babette, Couraud, Pierre-O, Kreppel, Florian, Kochanek, Stefan
    PLoS ONE, 2012;7(9):e45977.  2012
  25. Targeted protein engineering provides insights into binding mechanism and affinities of bacterial collagen adhesins.
    Authors: Ross C, Liang X, Liu Q, Murray B, Hook M, Ganesh V
    J Biol Chem, 2012;287(41):34856-65.  2012
  26. Characterization of the interface between normal and transformed epithelial cells.
    Authors: Hogan C, Dupre-Crochet S, Norman M, Kajita M, Zimmermann C, Pelling AE, Piddini E, Baena-Lopez LA, Vincent JP, Itoh Y, Hosoya H, Pichaud F, Fujita Y
    Nat. Cell Biol., 2009;11(4):460-7.  2009
  27. Biphasic modulation of paracellular claudin-5 expression in mouse brain endothelial cells is mediated through the phosphoinositide-3-kinase/AKT pathway.
    Authors: Camire R, Beaulac H, Brule S, McGregor A, Lauria E, Willis C
    J Pharmacol Exp Ther, 0;351(3):654-62.  0
  28. Mesencephalic Astrocyte-Derived Neurotrophic Factor as a Urine Biomarker for Endoplasmic Reticulum Stress-Related Kidney Diseases.
    Authors: Kim Y, Lee H, Manson S, Lindahl M, Evans B, Miner J, Urano F, Chen Y
    J Am Soc Nephrol, 0;27(10):2974-2982.  0
  29. Glycosylation at Asn211 regulates the activation state of the discoidin domain receptor 1 (DDR1).
    Authors: Fu H, Valiathan R, Payne L, Kumarasiri M, Mahasenan K, Mobashery S, Huang P, Fridman R
    J Biol Chem, 0;289(13):9275-87.  0
  30. Metabolite Profile and Antiproliferative Effects in HaCaT Cells of a Salix reticulata Extract.
    Authors: Corradi E, Schmidt N, Raber N, De Mieri M, Hamburger M, Butterweck V, Potterat O
    Planta Med, 0;83(14):1149-1158.  0
  31. RPTPalpha controls epithelial adherens junctions, linking E-cadherin engagement to c-Src-mediated phosphorylation of cortactin.
    Authors: Truffi M, Dubreuil V, Liang X, Vacaresse N, Nigon F, Han S, Yap A, Gomez G, Sap J
    J Cell Sci, 0;127(0):2420-32.  0

FAQs

  1. What is the difference between the standard Cultrex Rat Collagen I and the Cultrex 3-D Rat Collagen I?

    • Cultrex 3-D Rat Collagen I undergoes the same basic purification and efficacy tests as the standard collagen. However, it undergoes additional 3-D culture validation; it has been tested extensively for the ability to promote growth and differentiation of cell types, visualized by morphology in three dimensions in vitro.

  2. What are the differences between Cultrex Rat Collagen I and Cultrex Rat Collagen I, Low Viscosity?

    • Cultrex Rat Collagen I is provided at 5 mg/mL and is not pepsin treated, so it has intact telopeptides and is highly viscous. Cultrex Rat Collagen I, Low Viscosity is provided at 3 mg/mL, and it is easier to pipet. Both are provided in acetic acid and require pH neutralization prior to use. Please consult the provided product data sheets for pH neutralization instructions.

  3. Is Cultrex Rat Collagen I atelocollagen or telocollagen?

    • Cultrex Rat Collagen I is telocollagen.  

  4. After coating the Cultrex Rat Collagen I (catalog # 3440-100-01), there is a white film on the plate. What is this white film? Is this normal?

    • This white film is normal. The collagen coating can result in a slight white haziness to the plate, which could also be the result of excess salt if the plates are not well aspirated.

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