Collagens comprise a large family of insoluble extracellular glycoproteins that are essential components of connective tissues such as tendons, ligaments, cartilage, bone and skin. The mature polypeptides are secreted as coiled, left-handed helices that subsequently assemble into rope-like collagen fibers. Collagen I is a fibril-forming collagen that requires N- and C- terminal processing. Collagen IV is a network forming collagen whose C-terminus forms dimers and N-terminus forms tetramers.
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Cultrex Rat Collagen I
R&D Systems | Catalog # 3440-100-01
Purified stromal extracellular matrix protein that promotes cell adhesion for cell and tissue cultures
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Key Product Details
Features
Cultrex Rat Collagen I is a purified stromal extracellular matrix protein that promotes cell adhesion for cell and tissue culture. It may also be used to supplement customized coatings, hydrogels, bioinks, or medium formulations for cell culture.
Key Benefits
- Qualified for general cell culture applications
- May be used as a hydrogel or thin coating for tissue culture vessels
- Material provided at a standard concentration of ~5 mg/mL
Species
Rat
Product Summary for Cultrex Rat Collagen I
Why Use Cultrex Rat Collagen I?
Cultrex Rat Collagen I is purified from rat tail tendons and is provided at a high concentration that is capable of polymerizing to form a hydrogel. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37̊ C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I, a special process is employed to provide material at a standard concentration of approximately 5 mg/mL.
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Product Specifications
Source
Rat tail tendons
Protein Concentration
5 mg/mL
Endotoxin Level
≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Cell Culture Testing
Supports cell attachment and spreading of HT1080 human fibrosarcoma cells.
Viral Testing
No mycoplasma contamination detected by PCR.
Formulation, Preparation, and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at 2 - 8 °C.
Stability
Product is stable for a minimum of 2 months if stored at 2-8 °C. See lot specific Certificate of Analysis for expiration date.
Background: Collagen I
Alternate Names
Alpha-1 type I collagen, alpha1(I) procollagen, CAFYD, COL1A1, Collagen 1, collagen alpha 1 chain type I, collagen alpha-1(I) chain, collagen alpha-1(I) chain preproprotein, collagen of skin, tendon and bone, alpha-1 chain, collagen, type I, alpha 1, EDSARTH1, EDSC, OI1, OI2, OI3, OI4, pro-alpha-1 collagen type 1, type I proalpha 1, Type I Procollagen Alpha 1 Chain
Additional Collagen I Products
Product Documents for Cultrex Rat Collagen I
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Citations for Cultrex Rat Collagen I
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FAQs for Cultrex Rat Collagen I
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Q: After coating the Cultrex Rat Collagen I (catalog # 3440-100-01), there is a white film on the plate. What is this white film? Is this normal?
A: This white film is normal. The collagen coating can result in a slight white haziness to the plate, which could also be the result of excess salt if the plates are not well aspirated.
-
Q: Can the Organoid Harvesting Solution, Catalog # 3700-100-01, be used to dissociate cells grown on a substrate of Cultrex Rat Collagen 1?
A: The Organoid Harvesting Solution works well with BME (Basement Membrane Extract). For cells grown on Cultrex Rat Collagen 1, the recommended enzyme to use to dissociate cells is Collagenase. Collagenase treatment at 37oC for 30 minutes is recommended.
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Q: Is Cultrex Rat Collagen I atelocollagen or telocollagen?
A: Cultrex Rat Collagen I is telocollagen.
-
Q: What are the differences between Cultrex Rat Collagen I and Cultrex Rat Collagen I, Low Viscosity?
A: Cultrex Rat Collagen I is provided at 5 mg/mL and is not pepsin treated, so it has intact telopeptides and is highly viscous. Cultrex Rat Collagen I, Low Viscosity is provided at 3 mg/mL, and it is easier to pipet. Both are provided in acetic acid and require pH neutralization prior to use. Please consult the provided product data sheets for pH neutralization instructions.
-
Q: What is the difference between the standard Cultrex Rat Collagen I and the Cultrex 3-D Rat Collagen I?
A: Cultrex 3-D Rat Collagen I undergoes the same basic purification and efficacy tests as the standard collagen. However, it undergoes additional 3-D culture validation; it has been tested extensively for the ability to promote growth and differentiation of cell types, visualized by morphology in three dimensions in vitro.
-
Q: After coating the Cultrex Rat Collagen I (catalog # 3440-100-01), there is a white film on the plate. What is this white film? Is this normal?
A: This white film is normal. The collagen coating can result in a slight white haziness to the plate, which could also be the result of excess salt if the plates are not well aspirated.
-
Q: Can the Organoid Harvesting Solution, Catalog # 3700-100-01, be used to dissociate cells grown on a substrate of Cultrex Rat Collagen 1?
A: The Organoid Harvesting Solution works well with BME (Basement Membrane Extract). For cells grown on Cultrex Rat Collagen 1, the recommended enzyme to use to dissociate cells is Collagenase. Collagenase treatment at 37oC for 30 minutes is recommended.
-
Q: Is Cultrex Rat Collagen I atelocollagen or telocollagen?
A: Cultrex Rat Collagen I is telocollagen.
-
Q: What are the differences between Cultrex Rat Collagen I and Cultrex Rat Collagen I, Low Viscosity?
A: Cultrex Rat Collagen I is provided at 5 mg/mL and is not pepsin treated, so it has intact telopeptides and is highly viscous. Cultrex Rat Collagen I, Low Viscosity is provided at 3 mg/mL, and it is easier to pipet. Both are provided in acetic acid and require pH neutralization prior to use. Please consult the provided product data sheets for pH neutralization instructions.
-
Q: What is the difference between the standard Cultrex Rat Collagen I and the Cultrex 3-D Rat Collagen I?
A: Cultrex 3-D Rat Collagen I undergoes the same basic purification and efficacy tests as the standard collagen. However, it undergoes additional 3-D culture validation; it has been tested extensively for the ability to promote growth and differentiation of cell types, visualized by morphology in three dimensions in vitro.
-
Q: After coating the Cultrex Rat Collagen I (catalog # 3440-100-01), there is a white film on the plate. What is this white film? Is this normal?
A: This white film is normal. The collagen coating can result in a slight white haziness to the plate, which could also be the result of excess salt if the plates are not well aspirated.
-
Q: Can the Organoid Harvesting Solution, Catalog # 3700-100-01, be used to dissociate cells grown on a substrate of Cultrex Rat Collagen 1?
A: The Organoid Harvesting Solution works well with BME (Basement Membrane Extract). For cells grown on Cultrex Rat Collagen 1, the recommended enzyme to use to dissociate cells is Collagenase. Collagenase treatment at 37oC for 30 minutes is recommended.
-
Q: Is Cultrex Rat Collagen I atelocollagen or telocollagen?
A: Cultrex Rat Collagen I is telocollagen.
-
Q: What are the differences between Cultrex Rat Collagen I and Cultrex Rat Collagen I, Low Viscosity?
A: Cultrex Rat Collagen I is provided at 5 mg/mL and is not pepsin treated, so it has intact telopeptides and is highly viscous. Cultrex Rat Collagen I, Low Viscosity is provided at 3 mg/mL, and it is easier to pipet. Both are provided in acetic acid and require pH neutralization prior to use. Please consult the provided product data sheets for pH neutralization instructions.
-
Q: What is the difference between the standard Cultrex Rat Collagen I and the Cultrex 3-D Rat Collagen I?
A: Cultrex 3-D Rat Collagen I undergoes the same basic purification and efficacy tests as the standard collagen. However, it undergoes additional 3-D culture validation; it has been tested extensively for the ability to promote growth and differentiation of cell types, visualized by morphology in three dimensions in vitro.
-
Q: After coating the Cultrex Rat Collagen I (catalog # 3440-100-01), there is a white film on the plate. What is this white film? Is this normal?
A: This white film is normal. The collagen coating can result in a slight white haziness to the plate, which could also be the result of excess salt if the plates are not well aspirated.
-
Q: Can the Organoid Harvesting Solution, Catalog # 3700-100-01, be used to dissociate cells grown on a substrate of Cultrex Rat Collagen 1?
A: The Organoid Harvesting Solution works well with BME (Basement Membrane Extract). For cells grown on Cultrex Rat Collagen 1, the recommended enzyme to use to dissociate cells is Collagenase. Collagenase treatment at 37oC for 30 minutes is recommended.
-
Q: Is Cultrex Rat Collagen I atelocollagen or telocollagen?
A: Cultrex Rat Collagen I is telocollagen.
-
Q: What are the differences between Cultrex Rat Collagen I and Cultrex Rat Collagen I, Low Viscosity?
A: Cultrex Rat Collagen I is provided at 5 mg/mL and is not pepsin treated, so it has intact telopeptides and is highly viscous. Cultrex Rat Collagen I, Low Viscosity is provided at 3 mg/mL, and it is easier to pipet. Both are provided in acetic acid and require pH neutralization prior to use. Please consult the provided product data sheets for pH neutralization instructions.
-
Q: What is the difference between the standard Cultrex Rat Collagen I and the Cultrex 3-D Rat Collagen I?
A: Cultrex 3-D Rat Collagen I undergoes the same basic purification and efficacy tests as the standard collagen. However, it undergoes additional 3-D culture validation; it has been tested extensively for the ability to promote growth and differentiation of cell types, visualized by morphology in three dimensions in vitro.
-
Q: After coating the Cultrex Rat Collagen I (catalog # 3440-100-01), there is a white film on the plate. What is this white film? Is this normal?
A: This white film is normal. The collagen coating can result in a slight white haziness to the plate, which could also be the result of excess salt if the plates are not well aspirated.
-
Q: Can the Organoid Harvesting Solution, Catalog # 3700-100-01, be used to dissociate cells grown on a substrate of Cultrex Rat Collagen 1?
A: The Organoid Harvesting Solution works well with BME (Basement Membrane Extract). For cells grown on Cultrex Rat Collagen 1, the recommended enzyme to use to dissociate cells is Collagenase. Collagenase treatment at 37oC for 30 minutes is recommended.
-
Q: Is Cultrex Rat Collagen I atelocollagen or telocollagen?
A: Cultrex Rat Collagen I is telocollagen.
-
Q: What are the differences between Cultrex Rat Collagen I and Cultrex Rat Collagen I, Low Viscosity?
A: Cultrex Rat Collagen I is provided at 5 mg/mL and is not pepsin treated, so it has intact telopeptides and is highly viscous. Cultrex Rat Collagen I, Low Viscosity is provided at 3 mg/mL, and it is easier to pipet. Both are provided in acetic acid and require pH neutralization prior to use. Please consult the provided product data sheets for pH neutralization instructions.
-
Q: What is the difference between the standard Cultrex Rat Collagen I and the Cultrex 3-D Rat Collagen I?
A: Cultrex 3-D Rat Collagen I undergoes the same basic purification and efficacy tests as the standard collagen. However, it undergoes additional 3-D culture validation; it has been tested extensively for the ability to promote growth and differentiation of cell types, visualized by morphology in three dimensions in vitro.
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