CXC chemokine ligand 16 (CXCL16) is a type I membrane protein that has a non-ELR CXC chemokine domain in its extracellular region. Fractalkine (CX3CL1) and CXCL16 constitute the only two transmembrane chemokines. Mouse and human CXCL16 share 70% amino acid sequence similarity within their chemokine domains and 49% overall amino acid sequence identity.
CXCL16 Antibody (GT516) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-13519
![Immunohistochemistry-Paraffin: CXCL16 Antibody (GT516) [NBP3-13519]](https://resources.rndsystems.com/images/products/CXCL16-Antibody-GT516-Immunohistochemistry-Paraffin-NBP3-13519-img0002.jpg "Immunohistochemistry-Paraffin: CXCL16 Antibody (GT516) [NBP3-13519]")
Loading...
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # GT516
Format
Azide and BSA Free
Loading...
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human CXCL16. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
Centrifuge briefly prior to opening.
Scientific Data Images for CXCL16 Antibody (GT516) - Azide and BSA Free
Immunohistochemistry-Paraffin: CXCL16 Antibody (GT516) [NBP3-13519]
Immunohistochemistry-Paraffin: CXCL16 Antibody (GT516) [NBP3-13519] - CXCL16 antibody [GT516] detects CXCL16 protein at cytoplasm in rat lymph node by immunohistochemical analysis. Sample: Paraffin-embedded rat lymph node. CXCL16 antibody [GT516] (NBP3-13519) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: CXCL16 Antibody (GT516) [NBP3-13519] -
Western Blot: CXCL16 Antibody (GT516) [NBP3-13519] - HeLa whole cell extract and membrane extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CXCL16 antibody [GT516] (NBP3-13519) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)
Western Blot: CXCL16 Antibody (GT516) [NBP3-13519] -
Western Blot: CXCL16 Antibody (GT516) [NBP3-13519] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CXCL16 antibody [GT516] (NBP3-13519) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: CXCL16 Antibody (GT516) [NBP3-13519] -
Immunocytochemistry/ Immunofluorescence: CXCL16 Antibody (GT516) [NBP3-13519] - CXCL16 antibody [GT516] detects CXCL16 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CXCL16 stained by CXCL16 antibody [GT516] (NBP3-13519) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000.
Blue: Fluoroshield with DAPI.
Scale bar= 10um.
Western Blot: CXCL16 Antibody (GT516) [NBP3-13519] -
Western Blot: CXCL16 Antibody (GT516) [NBP3-13519] - HeLa whole cell and membrane extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CXCL16 antibody [GT516] (NBP3-13519) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Applications for CXCL16 Antibody (GT516) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CXCL16
Alternate Names
CXCL16
Gene Symbol
CXCL16
Additional CXCL16 Products
Product Documents for CXCL16 Antibody (GT516) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CXCL16 Antibody (GT516) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for CXCL16 Antibody (GT516) - Azide and BSA Free
There are currently no reviews for this product. Be the first to review CXCL16 Antibody (GT516) - Azide and BSA Free and earn rewards!
Have you used CXCL16 Antibody (GT516) - Azide and BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...
Associated Pathways
