Recombinant Bovine CXCL8/IL-8 Protein

Novus Biologicals | Catalog # NBP3-11063

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Key Product Details

Accession Number

P79255

Applications

Western Blot, ELISA
View all IL-8/CXCL8 Proteins and Enzymes »

Product Specifications

Description

Recombinant Bovine Interleukin-8 (IL-8) is a recombinant protein corresponding to aa 23-101 of native bovine IL-8.

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

9.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Bovine CXCL8/IL-8 Protein

ELISA: Recombinant Bovine CXCL8/IL-8 Protein [NBP3-11063]

ELISA: Recombinant Bovine CXCL8/IL-8 Protein [NBP3-11063]

ELISA: Recombinant Bovine CXCL8/IL-8 Protein [NBP3-11063] - Recombinant Bovine Interleukin-8 detected using Goat anti Bovine Interleukin-8 as the capture reagent and Goat anti Bovine Interleukin-8:Biotin as the detection reagent followed by Streptavidin:HRP.

Formulation, Preparation, and Storage

NBP3-11063
Preparation Method Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Formulation PBS
Preservative 10% Trehalose
Concentration Lyoph
Reconstitution Reconstitute with 20 uL sterile PBS containing 0.1% carrier protein. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-8/CXCL8

IL8 is a member of the CXC chemokine family. This family of small basic heparan-binding proteins are proinflammatory and primarily mediate the activation and migration of neutrophils into tissue from peripheral blood. This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL8 attracts neutrophils, basophils, and T-cells, but not monocytes. Cystic fibrosis (CF) is characterized by severe lung inflammation. The inflammatory process is believed to be caused by massive overproduction of the proinflammatory protein IL8, and the high levels of IL8 in the CF lung are therefore believed to be the central mechanism behind CF lung pathophysiology.

Long Name

Interleukin 8

Alternate Names

CXCL8, GCP1, IL8, LAI, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP1, NCF, TCF, TSG1

Gene Symbol

CXCL8

UniProt

P79255 (Bovine)

Additional IL-8/CXCL8 Products

Product Documents for Recombinant Bovine CXCL8/IL-8 Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Bovine CXCL8/IL-8 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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FAQs for Recombinant Bovine CXCL8/IL-8 Protein

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  • Q: Are mouse or rat CXCL8/IL-8 products offered?

    A: Mouse and rat do not have a gene which encodes CXCL8/IL-8. In mouse, the functional homologs to human CXCL8/IL-8 are CXCL1/KC and CXCL2/MIP-2. In rat, the functional homolog to CXCL8/human IL-8 is CXCL3/CINC-2.

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