CXCR1/IL-8RA Antibody (MM0221-7D22) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-12237

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Block/Neutralize, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2 Clone # MM0221-7D22

Format

Azide and BSA Free
View all CXCR1/IL-8RA Primary Antibodies »

Product Specifications

Immunogen

Human recombinant CXCR1/IL-8RA

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2

Scientific Data Images for CXCR1/IL-8RA Antibody (MM0221-7D22) - Azide and BSA Free

Flow Cytometry: CXCR1/IL-8RA Antibody (MM0221-7D22) - Azide and BSA Free [NBP2-12237]

Flow Cytometry: CXCR1/IL-8RA Antibody (MM0221-7D22) - Azide and BSA Free [NBP2-12237]

Flow Cytometry: CXCR1/IL-8 RA Antibody (MM0221-7D22) [NBP2-12237] - analysis of CXCR1 in human PBMC (bottom) using anti-CXCR1 antibody. Image from verified customer review.

Applications for CXCR1/IL-8RA Antibody (MM0221-7D22) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-200

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:20-1:100

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500-1:1000
Application Notes
This antibody is Cytof ready.

Reviewed Applications

Read 1 review rated 4 using NBP2-12237 in the following applications:

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCR1/IL-8RA

CXCR1, also known as IL-8RA and CD181, is an approximately 60 kDa 7TM glycoprotein that functions as a receptor for the chemokine CXCL8/IL-8. It is expressed on neutrophils, monocytes, CD8 T cells, FoxP3+ CD4 Treg cells, mast cells, neuronal and glial cells, vascular endothelial cells, and melanoma. CXCR1 forms homodimers and heterodimers with CXCR2/IL-8RB. It can be cleaved from neutrophils in the lungs of cystic fibrosis patients to release fragments that promote CXCL8 production from airway epithelial cells. CXCR1 mediates neutrophil activation and chemotaxis to sites of inflammation as well as angiogenesis and melanoma invasiveness.

Long Name

Interleukin 8 Receptor A

Alternate Names

CD181, IL-8 RA, IL8RA

Gene Symbol

CXCR1

UniProt

AAA59159

Additional CXCR1/IL-8RA Products

Product Documents for CXCR1/IL-8RA Antibody (MM0221-7D22) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CXCR1/IL-8RA Antibody (MM0221-7D22) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CXCR1/IL-8RA Antibody (MM0221-7D22) - Azide and BSA Free (1)

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  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: PBMC
    Species: Human
    Verified Customer | Posted 05/20/2015
    CXCR1_Rhesus Monkey PBMC
    CXCR1/IL-8RA Antibody (MM0221-7D22) - Azide and BSA Free NBP2-12237

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Protocols

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FAQs

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