Human CXCR4 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-06841

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

46.88 pg/mL

Assay Range

78.13 - 5000 pg/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human CXCR4 in samples. No significant cross-reactivity or interference between Human CXCR4 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 5.14

Inter-Assay Precision (Precision between assays) 5.67

Recovery for Human CXCR4 ELISA Kit (Colorimetric)

Recovery

85 - 108 %

Linearity

Human CXCR4 ELISA Kit (Colorimetric)

Scientific Data Images for Human CXCR4 ELISA Kit (Colorimetric)

Human CXCR4 ELISA Kit (Colorimetric)

ELISA: Human CXCR4 ELISA Kit (Colorimetric) [NBP3-06841] -

ELISA: Human CXCR4 ELISA Kit (Colorimetric) [NBP3-06841] - Standard Curve Reference

Kit Contents for Human CXCR4 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CXCR4

CD184, also known as fusin or CXCR4, is a 45 kD seven transmembrane G-protein-linked CXC chemokine receptor. CD184 is widely expressed on blood and tissue cells, including B and T cells, monocytes, macrophages, dendritic cells, granulocytes, megakaryocytes/platelets, lymphoid, myeloid precursor cells, endothelial cells, epithelial cells, astrocytes, and neurons, among other tissue cells. CD184 is the receptor for CXC chemokine SDF-1, mediates blood cell migration, and is involved in B lympho- and myelopoiesis, cardiogenesis, blood vessel formation and cerebellar development. CXCR4 is also a coreceptor of X4 HIV-1 and an alternative receptor for some isolates of HIV-2. The 12G5 antibody has been reported to block CD4-independent infection by HIV-2 and CD4-dependent infection by some T cell-tropic isolates of HIV-1.

Long Name

C-X-C Motif Chemokine Receptor 4

Alternate Names

CD184, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYRL, NPYY3R, WHIMS

Gene Symbol

CXCR4

Additional CXCR4 Products

Product Documents for Human CXCR4 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human CXCR4 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Human CXCR4 ELISA Kit (Colorimetric)

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  • Q: Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.

    A:

    CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

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