CXXC1 Antibody (PSH01-54)

Western Blot: CXXC1 Antibody (PSH01-54) [NBP3-32183]

Western Blot: CXXC1 Antibody (PSH01-54) [NBP3-32183] - Western blot analysis of CXXC1 on different lysates with Rabbit anti-CXXC1 antibody (NBP3-32183) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate (20 ug/Lane)
Lane 2: NIH/3T3 cell lysate (20 ug/Lane)
Lane 3: K-562 cell lysate (20 ug/Lane)
Lane 4: MCF7 cell lysate (20 ug/Lane)
Lane 5: RAW264.7 cell lysate (20 ug/Lane)
Lane 6: Mouse brain tissue lysate (40 ug/Lane)
Lane 7: L6 cell lysate (20 ug/Lane)
Lane 8: PC-12 cell lysate (20 ug/Lane)

Predicted band size: 76 kDa
Observed band size: 76 kDa

Exposure time: 5 minutes 10 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32183) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: CXXC1 Antibody (PSH01-54) [NBP3-32183]

Immunohistochemistry: CXXC1 Antibody (PSH01-54) [NBP3-32183] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-CXXC1 antibody (NBP3-32183) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32183) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.