CYBB/NOX2 Antibody (NL7) - BSA Free

Novus Biologicals | Catalog # NBP1-41012

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Flow (Intracellular)

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # NL7

Format

BSA Free
View all CYBB/NOX2 Primary Antibodies »

Product Specifications

Immunogen

Cytochrome B245 solubilized in octyl-beta-glucopyranoside

Epitope

Clone NL7 binds amino acids 498-506 (EKDVITGLK), a cytosolic epitope on gp91phox not accessible on intact neutrophils.

Specificity

This antibody recognises the heavy chain of cytochrome B245. It recognises the native as well as the denatured form of the protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CYBB/NOX2 Antibody (NL7) - BSA Free

Flow Cytometry: CYBB/NOX2 Antibody (NL7) - BSA Free [NBP1-41012]

Flow Cytometry: CYBB/NOX2 Antibody (NL7) - BSA Free [NBP1-41012]

Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on Daudi cells with CYBB/NOX2 (NL7) antibody NBP1-41012AF647 (blue) and a matched isotype control NBP1-97005AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: CYBB/NOX2 Antibody (NL7) - BSA Free [NBP1-41012]

Flow Cytometry: CYBB/NOX2 Antibody (NL7) - BSA Free [NBP1-41012]

Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Heavy Chain. Permeabilised with Leucoperm
Flow Cytometry: CYBB/NOX2 Antibody (NL7) - BSA Free [NBP1-41012]

Flow Cytometry: CYBB/NOX2 Antibody (NL7) - BSA Free [NBP1-41012]

Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Analysis using the PE conjugate of NBP1-41012. Staining of CYBB in human peripheral blood using anti-CYBB antibody. Image from verified customer review.
Flow (Intracellular): CYBB/NOX2 Antibody (NL7) - BSA Free [NBP1-41012]

Flow (Intracellular): CYBB/NOX2 Antibody (NL7) - BSA Free [NBP1-41012]

Flow (Intracellular): CYBB/NOX2 Antibody (NL7) [NBP1-41012] - An intracellular stain was performed on THP-1 cells with CYBB/NOX2 (NL7) antibody NBP1-41012AF647 (blue) and a matched isotype control(orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Applications for CYBB/NOX2 Antibody (NL7) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul (Burrit, J. B. et al.). Western blot detects a broad band at ~90 kDa. Clone NL7 has an inhibitory effect on NADPH oxidase activation. The antibody does not interfere with the translocation of the cytosolic subunits of NADPH oxidase to the cell membrane.

Reviewed Applications

Read 2 reviews rated 4.5 using NBP1-41012 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CYBB/NOX2

Cytochrome B245, also known as gp91phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterised by severe recurrent infections. The heavy chain (gp91phox), along with the light chain (p22phox) form cytochrome B245 (also known as flavocytochome b, or Cyt b), which is always membrane-resident. Upon the appropriate stimulus, other components of NADPH oxidase (p47phox, p40phox, p67phox and Rac2) rapidly translocate from the cytosol to the cell membrane to form the complete enzyme complex. Cytochrome B245 appears to serve as a scaffold for the assembly of the other cytosolic units of the oxidase and to provide a transmembrane pathway for electrons.

Long Name

Cytochrome b-245 heavy chain

Alternate Names

CGD91-phox, CYBB, EC 1.-.-.-, gp91-1, gp91-phox, NADPH oxidase 2, NOX2

Entrez Gene IDs

1536 (Human)

Gene Symbol

CYBB

UniProt

P04839 (Human)

Additional CYBB/NOX2 Products

Product Documents for CYBB/NOX2 Antibody (NL7) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CYBB/NOX2 Antibody (NL7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CYBB/NOX2 Antibody (NL7) - BSA Free

Customer Reviews for CYBB/NOX2 Antibody (NL7) - BSA Free (2)

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • CYBB/NOX2 Antibody (NL7)
    Name: James Petitte
    Application: Western Blot
    Sample Tested: Chicken cell lines and Mouse macrophages
    Species: Chicken and Mouse
    Verified Customer | Posted 12/06/2016
    Lanes 1,5 MQ-NCSU Chicken macrophage Lanes 2,4,6 overflow Lane 3 RAW Mouse Macrophage Lane 7 HD11 Chicken Macrophage Lane 8 Protein Ladder
    NSCS16 I do have a full experimental report that can be emailed. It works for mouse, but not chicken. We were hoping it would work for chicken.
    CYBB/NOX2 Antibody (NL7) - BSA Free NBP1-41012
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human placental membrane intact
    Species: Human
    Verified Customer | Posted 04/18/2013
    CYBB/NOX2 Antibody (NL7) - BSA Free NBP1-41012

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Protocols

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FAQs for CYBB/NOX2 Antibody (NL7) - BSA Free

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  • Q: I have a question about an antibody we have purchased and are using currently- NBP1-41012- CYBB/NOX2. My question is in regards to the packaging of this antibody. Is there a portion of BSA or is this antibody carrier free? I would like to proceed with con

    A: I have taken a look at the buffer components of NBP1-41012 can confirm that there is no BSA in the buffer. This anitbody is carrier free and can be conjugated. The buffer contains PBS and 0.09% sodium azide.

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