Cyclin B1 Antibody (1Q3E9)
Novus Biologicals | Catalog # NBP3-15626
Recombinant Monoclonal Antibody
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse
Applications
Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1Q3E9 expressed in HEK293
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Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 3-177 of human Cyclin B1 (P14635). LRVTRNSKINAENKAKINMAGAKRVPTAPAATSKPGLRPRTALGDIGNKVSEQLQAKMPMKKEAKPSATGKVIDKKLPKPLEKVPMLVPVPVSEPVPEPEPEPEPEPVKEEKLSPEPILVDTASPSPMETSGCAPAEEDLCQAFSDVILAVNDVDAEDGADPNLCSEYVKDIYAY
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Cyclin B1 Antibody (1Q3E9)
Western Blot: Cyclin B1 Antibody (1Q3E9) [NBP3-15626] -
Western Blot: Cyclin B1 Antibody (1Q3E9) [NBP3-15626] - Western blot analysis of lysates from wild type (WT) and Cyclin B1 knockout (KO) HeLa cells using [KO Validated] Cyclin B1 Rabbit mAb at 1:5000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 45s.
Western Blot: Cyclin B1 Antibody (1Q3E9) [Cyclin B1] -
Western Blot: Cyclin B1 Antibody (1Q3E9) [Cyclin B1] - Western blot analysis of lysates from Jurkat cells using [KO Validated] Cyclin B1 Rabbit mAb at 1:5000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 45s.
Applications for Cyclin B1 Antibody (1Q3E9)
Application
Recommended Usage
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Cyclin B1
Alternate Names
CCNB1
Gene Symbol
CCNB1
Additional Cyclin B1 Products
Product Documents for Cyclin B1 Antibody (1Q3E9)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Cyclin B1 Antibody (1Q3E9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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