Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Chicken (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cyclin C Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 244 and 294 of human cyclin C using the numbering given in entry NP_005181.2 (GeneID 892).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cyclin C Antibody - BSA Free

Western Blot: Cyclin C Antibody [NBP1-18878]

Western Blot: Cyclin C Antibody [NBP1-18878]

Western Blot: Cyclin C Antibody [NBP1-18878] - Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin C antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes.
Immunohistochemistry-Paraffin: Cyclin C Antibody [NBP1-18878]

Immunohistochemistry-Paraffin: Cyclin C Antibody [NBP1-18878]

Immunohistochemistry-Paraffin: Cyclin C Antibody [NBP1-18878] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Cyclin C used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: Cyclin C Antibody [NBP1-18878]

Immunoprecipitation: Cyclin C Antibody [NBP1-18878]

Immunoprecipitation: Cyclin C Antibody [NBP1-18878] - Detection of human Cyclin C by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Cyclin C antibody NBP1-18878 used for IP at 6 ug per reaction. For blotting immunoprecipitated Cyclin C, NBP1-18878 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Cyclin C Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000-1:5000

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 or Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cyclin C

The protein encoded by this gene is a member of the cyclin family of proteins. The encoded protein interacts with cyclin-dependent kinase 8 and induces the phophorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The level of mRNAs for this gene peaks in the G1 phase of the cell cycle. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

CycC, cyclin C, cyclin-C, hSRB11, SRB11 homolog

Entrez Gene IDs

892 (Human)

Gene Symbol

CCNC

UniProt

P24863 (Human)

Additional Cyclin C Products

Product Documents for Cyclin C Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Cyclin C Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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