Cyclin T2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87592

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cyclin T2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KAGSSKHHGPISTTPGIIPQKMSLDKYREKRKLETLDLDVRDHYIAAQVEQQHKQGQSQAASSSSVTSPIKMKIPIANTEKYMADKKEKSGSLKLRIPIPPTDKSASKEELKMKIKVSSSERHSSSDEGSGKSKHSSPH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cyclin T2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Cyclin T2 Antibody [NBP1-87592]

Immunocytochemistry/ Immunofluorescence: Cyclin T2 Antibody [NBP1-87592]

Immunocytochemistry/Immunofluorescence: Cyclin T2 Antibody [NBP1-87592] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cytosol.
Immunohistochemistry-Paraffin: Cyclin T2 Antibody [NBP1-87592]

Immunohistochemistry-Paraffin: Cyclin T2 Antibody [NBP1-87592]

Immunohistochemistry-Paraffin: Cyclin T2 Antibody [NBP1-87592] - Staining of human duodenum shows strong nuclear positivity in glandular cells.
Cyclin T2 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: Cyclin T2 Antibody - BSA Free [NBP1-87592]

Chromatin Immunoprecipitation-exo-Seq: Cyclin T2 Antibody - BSA Free [NBP1-87592]

ChIP-Exo-Seq composite graph for Anti-CCNT2 (NBP1-87592) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for Cyclin T2 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1-10µg per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP1-87592 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cyclin T2

Alternate Names

cyclin T2, cyclin T2a, cyclin T2b, cyclin-T2, CYCT2, FLJ13583, FLJ90560, MGC134840, SDS-stable vimentin-bound DNA fragment HEF42VIM22, subunit of positive elongation transcription factor b

Gene Symbol

CCNT2

Additional Cyclin T2 Products

Product Documents for Cyclin T2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Cyclin T2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cyclin T2 Antibody - BSA Free

Customer Reviews for Cyclin T2 Antibody - BSA Free (1)

5 out of 5
1 Customer Rating
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  • Cyclin T2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK 293 cell lysate
    Species: Mouse and Human
    Verified Customer | Posted 12/02/2016
    1. HEK293 2. HEK293 + Plasmid with other gene 3. HEK293 + Plasmid with other gene 4. HEK293 + Plasmid with other gene 5. HEK293 + Plasmid with other gene 6. HEK293 + Mouse CCNT2 plasmid 1ug 7. HEK293 + Mouse CCNT2 plasmid 4 ug
    Cyclin T2 Antibody - BSA Free NBP1-87592

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Protocols

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FAQs

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