CYP24A1 Antibody (1E1) - Azide and BSA Free

Novus Biologicals | Catalog # H00001591-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1E1

Format

Azide and BSA Free
View all CYP24A1 Primary Antibodies »

Product Specifications

Immunogen

CYP24A1 (NP_000773, 415 a.a. ~ 514 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LMLNTQVLGSSEDNFEDSSQFRPERWLQEKEKINPFAHLPFGVGKRMCIGRRLAELQLHLALCWIVRKYDIQATDNEPVEMLHSGTLVPSRELPIAFCQR

Reactivity Notes

Rat reactivity reported from customer review.

Specificity

CYP24A1 - cytochrome P450, family 24, subfamily A, polypeptide 1 (1E1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CYP24A1 Antibody (1E1) - Azide and BSA Free

Western Blot: CYP24A1 Antibody (1E1) [H00001591-M02]

Western Blot: CYP24A1 Antibody (1E1) [H00001591-M02]

Western Blot: CYP24A1 Antibody (1E1) [H00001591-M02] - Analysis of CYP24A1 expression in transfected 293T cell line by CYP24A1 monoclonal antibody (M02), clone 1E1.Lane 1: CYP24A1 transfected lysate (Predicted MW: 11.11 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: CYP24A1 Antibody (1E1) [H00001591-M02] - Detection limit for recombinant GST tagged CYP24A1 is approximately 1ng/ml as a capture antibody.

Applications for CYP24A1 Antibody (1E1) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Reviewed Applications

Read 1 review rated 3 using H00001591-M02 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CYP24A1

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial

Alternate Names

24-OHase, CYP24, EC 1.14.15.16

Entrez Gene IDs

1591 (Human)

Gene Symbol

CYP24A1

UniProt

NP_000773 (Human)

Additional CYP24A1 Products

Product Documents for CYP24A1 Antibody (1E1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CYP24A1 Antibody (1E1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CYP24A1 Antibody (1E1) - Azide and BSA Free

Customer Reviews for CYP24A1 Antibody (1E1) - Azide and BSA Free (1)

3 out of 5
1 Customer Rating
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell lysate, Sample Amount: 50ug-100ug protein
    Species: Other
    Verified Customer | Posted 03/25/2009

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FAQs

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