Cystatin F Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16074

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cystatin F Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cystatin F. The exact sequence is proprietary.

Localization

Secreted; Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cystatin F Antibody - BSA Free

Immunohistochemistry-Paraffin: Cystatin F Antibody [NBP2-16074]

Immunohistochemistry-Paraffin: Cystatin F Antibody [NBP2-16074]

Immunohistochemistry-Paraffin: Cystatin F Antibody [NBP2-16074] - Analysis of paraffin-embedded CL1-5 xenograft, using cystatin F antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Cystatin F Antibody

Immunocytochemistry/ Immunofluorescence: Cystatin F Antibody [NBP2-16074] -

Immunocytochemistry/ Immunofluorescence: Cystatin F Antibody [NBP2-16074] - Cystatin F antibody detects Cystatin F protein at vesicle by immunofluorescent analysis.
Sample: HL-60 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cystatin F stained by Cystatin F antibody (NBP2-16074) diluted at 1:500.
Blue: Hoechst 33342 staining.
Cystatin F Antibody

Western Blot: Cystatin F Antibody [NBP2-16074] -

Western Blot: Cystatin F Antibody [NBP2-16074] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cystatin F antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for Cystatin F Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cystatin F

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. This gene encodes a glycosylated cysteine protease inhibitor with a putative role in immune regulation through inhibition of a unique target in the hematopoietic system. Expression of the protein has been observed in various human cancer cell lines established from malignant tumors. [provided by RefSeq]

Alternate Names

CMAP, CST7, Cystatin 7, Leukocystatin

Gene Symbol

CST7

Additional Cystatin F Products

Product Documents for Cystatin F Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Cystatin F Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Cysteine Proteases and Regulators

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Protocols

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