Cytokeratin 14 Antibody (SPM263) [Allophycocyanin]

Name: Cytokeratin 14 Antibody (SPM263) [Allophycocyanin]
Brand: Novus Biologicals
SKU: NBP2-34403APC
Price: 549 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-34403APC

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Product Documents

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Product Specifications
Scientific Data
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # SPM263

View all Cytokeratin 14 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide of 15 amino acid from the C-terminus of human Cytokeratin 14. (Uniprot: P02533)

Localization

Cytoplasmic

Marker

Squamous Cell Marker

Specificity

Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Description

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Scientific Data Images for Cytokeratin 14 Antibody (SPM263) [Allophycocyanin]

Cytokeratin-14-Antibody-SPM263-Allophycocyanin-Flow-Cytometry-NBP2-34403APC-img0005.jpg

Flow Cytometry: Cytokeratin 14 Antibody (SPM263) [Allophycocyanin] [NBP2-34403APC] - An intracellular stain was performed on HeLa cells with Cytokeratin 14 (SPM263) antibody NBP2-34403APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin.

Cytokeratin-14-Antibody-SPM263-Allophycocyanin-Flow-Cytometry-NBP2-34403APC-img0006.jpg

Flow Cytometry: Cytokeratin 14 Antibody (SPM263) [Allophycocyanin] [NBP2-34403APC] -

Flow Cytometry: Cytokeratin 14 Antibody (SPM263) [Allophycocyanin] [NBP2-34403APC] - HBLAK contains two cell populations. (a) HBLAK cells homogeneously express CD90 & KRT5, but two subpopulations are distinguishable by size & KRT14 staining; namely, FSC-Alow/KRT14high & FSC-Ahigh/KRT14low. (b) The larger KRT14low cells are more intensely labeled by EdU staining. (c) The FSC-Ahigh/KRT14low population is additionally distinguishable by high AldeFluor-assay activity. In (a–c), evaluated cells are circled. (d) Effect of UTX knockdown using siRNA 01 on the proportion of the AldeFluorpos population. As a negative control, DEAB reagent (inhibitor of the AldeFluor-assay) was used. Significant differences were observed 4 & 7 days after transfection of control siRNA or UTX-siRNA. Number of analyzed independent experiments, n = 4. Statistics were performed with a one-way ANOVA with a post-hoc Tukey HSD test (# p < 0.05). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32326336), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Flow Cytometry: Cytokeratin 14 Antibody (SPM263) [Allophycocyanin] [NBP2-34403APC] -

Flow Cytometry: Cytokeratin 14 Antibody (SPM263) [Allophycocyanin] [NBP2-34403APC] - Enrichment of KRT14high cells after UTX knockdown in HBLAK cells. (a) Immunocytochemical detection of KRT5 & KRT14, nuclei with DAPI & F-actin with phalloidin after transfection with UTX-siRNA for 2, 4 or 7 days. (b) Quantification of KRT14high cells via a cell structure-related signal threshold analysis (ImageJ) in the HBLAK population following UTX knockdown. At least three experiments with >50 cells each were evaluated for each treatment & time point. The percentage of KRT14high cells was measured via ImageJ analysis, as described in the Methods section. (c) KRT14 expression as detected by flow cytometry on day 4 after transfection of control siRNA or UTX-siRNA (n = 4). Statistics were performed with a two-way ANOVA with a post-hoc Tukey HSD test (## p < 0.01; ### p < 0.001). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32326336), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

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