Cytokeratin 17 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32824

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cytokeratin 17 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 17. The exact sequence is proprietary.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cytokeratin 17 Antibody - BSA Free

Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody [NBP1-32824]

Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody [NBP1-32824]

Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody [NBP1-32824] - Cytokeratin 17 antibody detects Cytokeratin 17 protein at cytoplasm in human cervical metaplasia (left) and carcinoma (right) by immunohistochemical analysis.Sample: Paraffin-embedded human cervix. Antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Cytokeratin 17 Antibody [NBP1-32824]

Western Blot: Cytokeratin 17 Antibody [NBP1-32824]

Western Blot: Cytokeratin 17 Antibody [NBP1-32824] - Sample (50 ug of whole cell lysate) A: Rat bladder 10% SDS PAGE; antibody diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 17 Antibody [NBP1-32824]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 17 Antibody [NBP1-32824]

Immunocytochemistry/Immunofluorescence: Cytokeratin 17 Antibody [NBP1-32824] - Paraformaldehyde-fixed HeLa, using Cytokeratin 17 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2500.
Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody [NBP1-32824]

Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody [NBP1-32824]

Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody [NBP1-32824] - Paraffin-embedded N87 xenograft, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody [NBP1-32824]

Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody [NBP1-32824]

Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody [NBP1-32824] - Cytokeratin 17 antibody detects Cytokeratin 17 protein at cytoplasm on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus.Cytokeratin 17 antibody diluted at 1:500. Antigen Retrieval: Trilogy (TM) (EDTA based, pH 8.0) buffer, 15min
Cytokeratin 17 Antibody - BSA Free

Western Blot: Cytokeratin 17 Antibody [NBP1-32824] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 17 (NBP1-32824) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for Cytokeratin 17 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cytokeratin 17

Cytokeratins, a group of at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 4, 5, 6 and 8 are members of the type II neutral-to-basic subfamily. Cytokeratin peptide 4 (59 kDa) is the secondary type II keratin expressed in non cornified stratified squamous epithelia. Cytokeratin peptide 5 (58 kDa) is the primary type II keratin in stratified epithelia, while cytokeratin type 8 (52 kDa) is a major type II keratin in simple epithelia. Cytokeratin 6 (56 kDa) is a "hyperproliferation" cytokeratin expressed in tissues with natural or pathological high turnover. Cytokeratins 10, 13 and 18 are members of the type I acidic subfamily. Cytokeratin peptide 10 (56 kDa) is the secondary type I keratin expressed in cornified epithelia. Cytokeratin 13 (54 kDa) is the secondary type I keratin expressed in non-cornified stratified squamous epithelia. Cytokeratin 18 (45 kDa) is the primary type I keratin expressed in simple epithelial cells. Cytokeratin is a heterotetramer of two type I and two type II keratins. Keratin 1 is generally associated with keratin 10. Defects in Cytokeratin are a cause of epidermolytic hyperkeratosis, also known as bullous congenital ichthyosiform erythroderma (BIE), which is a hereditary skin disorder characterized by blistering and a marked thickening of the stratum corneum. Defects in Cytokeratin are also the cause of Curth Macklin type ichthyosis hystrix, nonepidermolytic palmoplantar keratoderma, annular epidermolytic ichthyosis and keratosis palmoplantaris striata III (PPKS3). PPKS3 is an autosomal dominant disorder affecting palm and sole skin where stratum corneum and epidermal layers are thickened. There is no involvement of nonpalmoplantar skin, and both hair and nails are normal.

Long Name

Cytokeratin 17/KRT17

Alternate Names

CK-17, cytokeratin-17, K17, keratin 17, type I, keratin, type I cytoskeletal 17, PCHC1

Gene Symbol

KRT17

Additional Cytokeratin 17 Products

Product Documents for Cytokeratin 17 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Cytokeratin 17 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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