Cytokeratin 18 Antibody (KRT18/835)

Novus Biologicals | Catalog # NBP2-44949

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Bovine (Negative), Canine (Negative), Hamster (Negative), Porcine (Negative), Rat (Negative), Sheep (Negative)

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # KRT18/835
View all Cytokeratin 18 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Cytokeratin 18 protein (Uniprot: P05783)

Reactivity Notes

Does not react with Rat, Sheep, Hamster, Bovine, Canine or Porcine.

Localization

Cytoplasmic

Marker

Epithelial Marker

Specificity

This monoclonal antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47986)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin 18 Antibody (KRT18/835)

Western Blot: Cytokeratin 18 Antibody (KRT18/835) [NBP2-44949]

Western Blot: Cytokeratin 18 Antibody (KRT18/835) [NBP2-44949]

Western Blot: Cytokeratin 18 Antibody (KRT18/835) [NBP2-44949] - Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Antibody (KRT18/835).
Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (KRT18/835) [NBP2-44949]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (KRT18/835) [NBP2-44949]

Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (KRT18/835) [NBP2-44949] - Confocal immunofluorescence image of MCF cells using Cytokeratin 18 Antibody (KRT18/835) Green (CF488) and Red Dot is used to label the nuclei.
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/835) [NBP2-44949]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/835) [NBP2-44949]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/835) [NBP2-44949] - Human Thyroid Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/835).
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/835) [NBP2-44949]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/835) [NBP2-44949]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/835) [NBP2-44949] - Human Bladder Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/835).
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/835) [NBP2-44949]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/835) [NBP2-44949]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/835) [NBP2-44949] - Human Colon Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/835).
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/835) [NBP2-44949]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/835) [NBP2-44949]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/835) [NBP2-44949] - Human Lung Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/835).
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/835) [NBP2-44949]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/835) [NBP2-44949]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/835) [NBP2-44949] - Human Prostate Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/835).
Protein Array: Cytokeratin 18 Antibody (KRT18/835) [NBP2-44949]

Protein Array: Cytokeratin 18 Antibody (KRT18/835) [NBP2-44949]

Protein Array: Cytokeratin 18 Antibody (KRT18/835) [NBP2-44949] - Analysis of Protein Array containing >19,000 full-length human proteins using Cytokeratin 18 Antibody (KRT18/835) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for Cytokeratin 18 Antibody (KRT18/835)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 18

Cytokeratins are cytoskeletal intermediate filament proteins that are classified as type I (acidic) and type II (neutral to basic) and associate into heterotetramers.

Alternate Names

CK-18, CYK18, Keratin 18, KRT18

Gene Symbol

KRT18

UniProt

P05783 (Human)

Additional Cytokeratin 18 Products

Product Documents for Cytokeratin 18 Antibody (KRT18/835)

Certificate of Analysis

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Product Specific Notices for Cytokeratin 18 Antibody (KRT18/835)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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