Cytokeratins are cytoskeletal intermediate filament proteins that are classified as type I (acidic) and type II (neutral to basic) and associate into heterotetramers.
Cytokeratin 18 Antibody (RGE53) - BSA Free
Novus Biologicals | Catalog # NBP1-97715
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-RGE53-Immunocytochemistry-Immunofluorescence-NBP1-97715-img0006.jpg "Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Canine, Chicken, Guinea Pig, Hamster, Rabbit, Zebrafish
Cited:
Porcine, Canine, Guinea Pig
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Cited:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # RGE53
Format
BSA Free
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Product Specifications
Immunogen
Derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal preparation of HeLa cells.
Reactivity Notes
Use in Guinea Pig reported in scientific literature (PMID:10.1186/s40850-021-00074-w). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Marker
Epithelial Marker
Specificity
This antibody reacts exclusively with Cytokeratin 18 which is present in glandular epithelial cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Scientific Data Images for Cytokeratin 18 Antibody (RGE53) - BSA Free
Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]
Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of 1 month old zebrafish embryo![Immunohistochemistry: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-RGE53-Immunohistochemistry-NBP1-97715-img0007.jpg "Immunohistochemistry: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]")
Immunohistochemistry: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]
Immunohistochemistry: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Immunofluorescence staining of human colon epithelium.![Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-RGE53-Immunocytochemistry-Immunofluorescence-NBP1-97715-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]")
Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]
Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of a 2 days old zebrafish embryo. Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining![Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-RGE53-Immunocytochemistry-Immunofluorescence-NBP1-97715-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]")
Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]
Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of a 5 days old zebrafish embryo. Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining![Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-RGE53-Immunohistochemistry-Frozen-NBP1-97715-img0002.jpg "Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]")
Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]
Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Frozen section of human colon![Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-RGE53-Immunohistochemistry-Frozen-NBP1-97715-img0001.jpg "Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]")
Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]
Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Frozen section of human kidney epithelium![Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-RGE53-Immunohistochemistry-Frozen-NBP1-97715-img0003.jpg "Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]")
Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) - BSA Free [NBP1-97715]
Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Frozen section of swine liver hepatocytesApplications for Cytokeratin 18 Antibody (RGE53) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:100-1:200
Western Blot
1:100-1:1000
Application Notes
This antibody is suitable for immunoblotting, immunocytochemistry on acetone fixed cells, immunohistochemistry on frozen sections and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
This antibody is CyTOF ready..
This antibody is CyTOF ready..
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS, pH 7.2
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Cytokeratin 18
Alternate Names
CK-18, CYK18, Keratin 18, KRT18
Gene Symbol
KRT18
Additional Cytokeratin 18 Products
Product Documents for Cytokeratin 18 Antibody (RGE53) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Cytokeratin 18 Antibody (RGE53) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Cytokeratin 18 Antibody (RGE53) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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