Cytokeratins are cytoskeletal intermediate filament proteins that are classified as type I (acidic) and type II (neutral to basic) and associate into heterotetramers.
Cytokeratin 18 Antibody (SZ80-07)
Novus Biologicals | Catalog # NBP2-67370
Recombinant Monoclonal Antibody
![Western Blot: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-SZ80-07-Western-Blot-NBP2-67370-img0011.jpg "Western Blot: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SZ80-07 expressed in HEK293
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Product Specifications
Immunogen
Recombinant protein within mouse Cytokeratin 18 aa 200-423. (SwissProt: P05783 Human; SwissProt: P05784 Mouse; SwissProt: Q5BJY9 Rat)
Localization
Cytoplasm, Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Cytokeratin 18 Antibody (SZ80-07)
Western Blot: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]
Western Blot: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Western blot analysis of Cytokeratin 18 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat![Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-SZ80-07-Immunocytochemistry-Immunofluorescence-NBP2-67370-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]")
Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]
Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Staining Cytokeratin 18 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0016.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]")
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human liver tissue with Rabbit anti-Cytokeratin 18 antibody washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.![Flow Cytometry: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-SZ80-07-Flow-Cytometry-NBP2-67370-img0001.jpg "Flow Cytometry: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]")
Flow Cytometry: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]
Flow Cytometry: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of MCF-7 cells with Cytokeratin 18 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody![Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-SZ80-07-Immunocytochemistry-Immunofluorescence-NBP2-67370-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]")
Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]
Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Staining Cytokeratin 18 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-SZ80-07-Immunocytochemistry-Immunofluorescence-NBP2-67370-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]")
Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]
Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Staining Cytokeratin 18 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0005.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]")
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0006.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]")
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human colon cancer tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0007.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]")
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human gastric carcinoma tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0008.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]")
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human kidney tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0009.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]")
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0012.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]")
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human colon tissue with Rabbit anti-Cytokeratin 18 antibody washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0013.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]")
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-Cytokeratin 18 antibody washed with ddH2O and PBS, and then probed with the primary antibody 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0014.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]")
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-Cytokeratin 18 antibody washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0015.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]")
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370]
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human colon carcinoma with Rabbit anti-Cytokeratin 18 antibody washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Applications for Cytokeratin 18 Antibody (SZ80-07)
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:2000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Cytokeratin 18
Alternate Names
CK-18, CYK18, Keratin 18, KRT18
Gene Symbol
KRT18
Additional Cytokeratin 18 Products
Product Documents for Cytokeratin 18 Antibody (SZ80-07)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Cytokeratin 18 Antibody (SZ80-07)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Cytokeratin 18 Antibody (SZ80-07)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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