Cytokeratin 19 Antibody (MSVA-119M)

Novus Biologicals | Catalog # NBP3-57207

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Lambda/IgG1 Kappa Clone # MSVA-119M
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Product Specifications

Immunogen

Recombinant human KRT19 protein Swissprot P08727

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Lambda/IgG1 Kappa

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.

Positive Control
Postive control:Colon: A strong immunostaining is seen in all epithelial cells. Tonsil: A moderate to strong immunostaining occurs in the basal cell layer of the squamous epithelium of the tonsil surface..

Negative Control
Negitve control:Colon: All non-epithelial cells are CK19 negative. Tonsil: All non-basal squamous epithelial cells and all non-epithelial cells are CK19 negative.

Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Cytokeratin 19 Antibody (MSVA-119M)

Cytokeratin 19 Antibody (MSVA-119M)

Immunohistochemistry-Paraffin:Cytokeratin 19 Antibody (MSVA-119M)NBP3-57207

Malignant mesothelioma with strong cytokeratin 19 positivity.
Cytokeratin 19 Antibody (MSVA-119M)

Immunohistochemistry-Paraffin:Cytokeratin 19 Antibody (MSVA-119M)NBP3-57207

Ectocervix In non keratinizing squamous epithelium the basal cells are CK19 positive while the remaining layers are usually negative
Cytokeratin 19 Antibody (MSVA-119M)

Immunohistochemistry-Paraffin:Cytokeratin 19 Antibody (MSVA-119M)NBP3-57207

Appendix mucosa 1 20
Cytokeratin 19 Antibody (MSVA-119M) Cytokeratin 19 Antibody (MSVA-119M) Cytokeratin 19 Cancer Tissue Gallery

Cytokeratin 19 Cancer Tissue Gallery

Cytokeratin 19 Antibody (MSVA-119M) Cytokeratin 19 Antibody (MSVA-119M) Cytokeratin 19 Normal Tissue Gallery

Cytokeratin 19 Normal Tissue Gallery

Cytokeratin 19 Antibody (MSVA-119M) Cytokeratin 19 Antibody (MSVA-119M) Cytokeratin 19 Normal Tissue Gallery

Cytokeratin 19 Normal Tissue Gallery

Cytokeratin 19 Antibody (MSVA-119M) Cytokeratin 19 Antibody (MSVA-119M) Cytokeratin 19 Normal Tissue Gallery

Cytokeratin 19 Normal Tissue Gallery

Cytokeratin 19 Antibody (MSVA-119M) Cytokeratin 19 Antibody (MSVA-119M) Cytokeratin 19 Normal Tissue Gallery

Cytokeratin 19 Normal Tissue Gallery

Cytokeratin 19 Antibody (MSVA-119M) Cytokeratin 19 Antibody (MSVA-119M) Cytokeratin 19 Normal Tissue Gallery

Cytokeratin 19 Normal Tissue Gallery

Applications for Cytokeratin 19 Antibody (MSVA-119M)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 19

Cytokeratin 19 (also known as Keratin, type I cytoskeletal 19) belongs to the keratin family which consists of fibrous proteins that make up the framework of epithelial cells. Cytokeratin 19 plays a role in the organization of myofibers. This protein is known to have interactions with ZNF638, FANCG, FAM107A, KRT15 and EXOC8. Cytokeratin 19 has been studied in relation to several disorders and diseases including chordoma, adenoma, hamartoma, adenoiditis, carcinoma, and liver disease.

Alternate Names

CK19, EndoC, K19, Krt19

Gene Symbol

KRT19

Additional Cytokeratin 19 Products

Product Documents for Cytokeratin 19 Antibody (MSVA-119M)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Cytokeratin 19 Antibody (MSVA-119M)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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