Cytokeratin 2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-55126

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cytokeratin 2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to KRT2A The peptide sequence was selected from the middle region of KRT2A. Peptide sequence EVKAQYEEIAQRSKEEAEALYHSKYEELQVTVGRHGDSLKEIKIEISELN. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Cytokeratin 2 Antibody - BSA Free

Western Blot: Cytokeratin 2 Antibody [NBP1-55126]

Western Blot: Cytokeratin 2 Antibody [NBP1-55126]

Western Blot: Cytokeratin 2 Antibody [NBP1-55126] - Titration: 0.2-1 ug/ml Positive Control: Jurkat cell lysate.
Immunohistochemistry: Cytokeratin 2 Antibody [NBP1-55126]

Immunohistochemistry: Cytokeratin 2 Antibody [NBP1-55126]

Immunohistochemistry: Cytokeratin 2 Antibody [NBP1-55126] - Human alveolar cell Cellular data: Epithelial cells of renal tubule Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X.
Immunohistochemistry-Paraffin: Cytokeratin 2 Antibody [NBP1-55126]

Immunohistochemistry-Paraffin: Cytokeratin 2 Antibody [NBP1-55126]

Immunohistochemistry-Paraffin: Cytokeratin 2 Antibody [NBP1-55126] - Human Intestine Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Epithelial cells of intestinal villus (indicated with arrows) 400X magnification.

Applications for Cytokeratin 2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 2

KRT2A is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma.The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Alternate Names

CK-2e, Cytokeratin-2e, epidermal ichthyosis bullosa of Siemens, Epithelial keratin-2e, K2e, keratin 2, keratin 2A (epidermal ichthyosis bullosa of Siemens), keratin, type II cytoskeletal 2 epidermal, Keratin-2 epidermis, keratin-2e, KRT2AMGC116967, KRT2EMGC116968, KRTE, Type-II keratin Kb2

Gene Symbol

KRT2

Additional Cytokeratin 2 Products

Product Documents for Cytokeratin 2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Cytokeratin 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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