Cytokeratin 20 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85600

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cytokeratin 20 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KEHQEEVDGLHKHLGNTVNVEVDAAPGLNLGVIMNEMRQKYEVMAQKNLQEAKEQFERQTAVLQQQVTVNTEELKGTEVQLTELRRTSQ

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cytokeratin 20 Antibody - BSA Free

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody [NBP1-85600]

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody [NBP1-85600]

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody [NBP1-85600] - Staining in human small intestine and liver tissues using anti-KRT20 antibody. Corresponding KRT20 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody [NBP1-85600]

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody [NBP1-85600]

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody [NBP1-85600] - Staining of human small intestine shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody [NBP1-85600]

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody [NBP1-85600]

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody [NBP1-85600] - Staining of human liver shows low expression as expected.
Cytokeratin 20 Antibody - BSA Free Western Blot: Cytokeratin 20 Antibody - BSA Free [NBP1-85600]

Western Blot: Cytokeratin 20 Antibody - BSA Free [NBP1-85600]

Analysis in control (vector only transfected HEK293T lysate) and KRT20 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for Cytokeratin 20 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 20

Intermediate-sized filament (IF) protein designated cytokeratin (CK) 1 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract (1). Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium (2). CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin (3).

Alternate Names

CD20, CK20, CK-20, cytokeratin-20, K20cytokeratin 20, keratin 20, keratin, type I cytoskeletal 20, keratin-20, KRT21, MGC35423, Protein IT

Gene Symbol

KRT20

Additional Cytokeratin 20 Products

Product Documents for Cytokeratin 20 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Cytokeratin 20 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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