Cytokeratin 5/14 Antibody (16.4) - BSA Free

Novus Biologicals | Catalog # NBP2-66615

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 16.4

Format

BSA Free
View all Cytokeratin 5/14 Primary Antibodies »

Product Specifications

Immunogen

Hybridoma produced by the fusion of splenocytes from mice immunized with cytokeratin enriched extract of feline tongue epithelium and mouse myeloma cells.

Specificity

Detects Keratin 5 and 14 by Western blot. Detects a 58 kDa band, corresponding to Keratin 5, and a 50 kDa band, corresponding to Keratin 14.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Cytokeratin 5/14 Antibody (16.4) - BSA Free

Application
Recommended Usage

Immunohistochemistry-Frozen

1-5 ug/ml
Application Notes
Does not work on paraffin embedded tissue sections. Optimal concentration should be evaluated by serial dilutions..

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.08% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytokeratin 5/14

Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Belonging to the type A (acidic) subfamily of low molecular weight keratins and existing in combination with keratin 5, keratin 14 distinguishes stratified epithelial cells from simple epithelial cells and is useful in identification of squamous cell carcinomas. It is considered a prognostic marker in breast carcinomas.

Alternate Names

58 kDa cytokeratin, CK5, CK-5, Cytokeratin-5, DDD, EBS2, epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types, K5, keratin 5, keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockaynetypes), keratin, type II cytoskeletal 5, Keratin-5, KRT5A, Type-II keratin Kb5

Gene Symbol

KRT5

UniProt

P02533 (Human)

Additional Cytokeratin 5/14 Products

Product Documents for Cytokeratin 5/14 Antibody (16.4) - BSA Free

Certificate of Analysis

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Product Specific Notices for Cytokeratin 5/14 Antibody (16.4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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