Cytokeratin 5/14 Antibody (XM26/LL002)

Novus Biologicals | Catalog # NB110-59922

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1/IgG3 Clone # XM26/LL002
View all Cytokeratin 5/14 Primary Antibodies »

Product Specifications

Immunogen

XM26: prokaryotic recombinant protein corresponding to 103 amino acid portion of C-terminal region of the human cytokeratin 5 molecule. LL002: Synthetic peptide of the extreme c-terminal of human keratin-14

Localization

Cytoplasmic

Specificity

This antibody cocktail reacts with cytokeratin 5 intermediate filament protein as well as to 50 kD cytokeratin protein designated cytokeratin 14. Basal cells of human epidermis express acidic cytokeratin 14 and basic cytokeratin 5. Cytokeratin 5 is a 58 kD protein which is closely related to cytokeratin 6. Cytokeratin 5 and 6 are expressed in basal cell epithelioma, basal cells of prostate, urothelium, vagina, squamous cell carcinomas of skin, tongue, epiglottis, and in the rectal-anal region. Cytokeratin 5 can be useful in the destinction of mesotheliomas from most adenocarcinomas. Cytokeratin 14 belongs to the type A (acidic) subfamily of low MW cytokeratins and exists in combination with cytokeratin 15. This antibody can be useful to distinguish stratified ephithelial cells from simple epithelial cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1/IgG3

Description

This product has a prediluted size available. If a 6mL or 7mL pre-diluted size is purchased this product can be used Neat (without further dilution). Use recommended dilution for the concentrated vials only.

Scientific Data Images for Cytokeratin 5/14 Antibody (XM26/LL002)

Immunohistochemistry-Paraffin: Cytokeratin 5/14 Antibody (XM26/LL002) [NB110-59922]

Immunohistochemistry-Paraffin: Cytokeratin 5/14 Antibody (XM26/LL002) [NB110-59922]

Immunohistochemistry-Paraffin: Cytokeratin 5/14 Antibody (XM26 & LL002) [NB110-59922] - Formalin fixed paraffin embedded human tonsil stained with Cytokeratin 5/14 (NB110-59922).

Applications for Cytokeratin 5/14 Antibody (XM26/LL002)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:25-1:75

Immunohistochemistry-Paraffin

1:25-1:75
Application Notes
IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.4), 0.2% BSA, Tween-20

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 5/14

Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Belonging to the type A (acidic) subfamily of low molecular weight keratins and existing in combination with keratin 5, keratin 14 distinguishes stratified epithelial cells from simple epithelial cells and is useful in identification of squamous cell carcinomas. It is considered a prognostic marker in breast carcinomas.

Alternate Names

58 kDa cytokeratin, CK5, CK-5, Cytokeratin-5, DDD, EBS2, epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types, K5, keratin 5, keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockaynetypes), keratin, type II cytoskeletal 5, Keratin-5, KRT5A, Type-II keratin Kb5

Entrez Gene IDs

3852 (Human)

Gene Symbol

KRT5

Additional Cytokeratin 5/14 Products

Product Documents for Cytokeratin 5/14 Antibody (XM26/LL002)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Cytokeratin 5/14 Antibody (XM26/LL002)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cytokeratin 5/14 Antibody (XM26/LL002)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Cytokeratin 5/14 Antibody (XM26/LL002)

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  • Q: Has this antibody been tested on mouse tissues? Was there any cross-reactivity? 

    A: This antibody has not been tested on mouse tissues, and is only guaranteed to work for human samples.

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