Cytokeratin 7/17 Antibody (C-46) - BSA Free

Novus Biologicals | Catalog # NB500-352

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Porcine, Bovine, Mouse (Negative), Rabbit (Negative), Rat (Negative)

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # C-46

Format

BSA Free
View all Cytokeratin 7/17 Primary Antibodies »

Product Specifications

Immunogen

Cytoskeleton preparation of HeLa human cervix carcinoma cell line.

Specificity

The antibody C-46 reacts with Cytokeratin peptides 7 and 17 (54 and 46 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytokeratin 7/17 Antibody (C-46) - BSA Free

Western Blot: Cytokeratin 7/17 Antibody (C-46)BSA Free [NB500-352]

Western Blot: Cytokeratin 7/17 Antibody (C-46)BSA Free [NB500-352]

Western Blot: Cytokeratin 7/17 Antibody (C-46) [NB500-352] - Detection of Cytokeratin 7+17 in HeLa cell lysate with monoclonal antibody C-46.
Immunohistochemistry-Paraffin: Cytokeratin 7/17 Antibody (C-46) - BSA Free [NB500-352]

Immunohistochemistry-Paraffin: Cytokeratin 7/17 Antibody (C-46) - BSA Free [NB500-352]

Immunohistochemistry-Paraffin: Cytokeratin 7/17 Antibody (C-46) [NB500-352] - Staining of human breast (paraffin-embedded sections) with anti-cytokeratin 7+17 (C-46).
Flow (Intracellular): Cytokeratin 7/17 Antibody (C-46) - BSA Free [NB500-352]

Flow (Intracellular): Cytokeratin 7/17 Antibody (C-46) - BSA Free [NB500-352]

Flow (Intracellular): Cytokeratin 7/17 Antibody (C-46) [NB500-352] - An intracellular stain was performed on HeLa cells with Cytokeratin 7/17 (C-46) antibody NB500-352AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Immunohistochemistry: Cytokeratin 7/17 Antibody (C-46) - BSA Free [NB500-352]

Immunohistochemistry: Cytokeratin 7/17 Antibody (C-46) - BSA Free [NB500-352]

Immunohistochemistry: Cytokeratin 7/17 Antibody (C-46) [NB500-352] - Fig. 1. Immunohistochemistry staining of pancreas (paraffin-embedded sections) with anti-Cytokeratin 7/17 Antibody (C-46).
Flow Cytometry: Cytokeratin 7/17 Antibody (C-46) - BSA Free [NB500-352]

Flow Cytometry: Cytokeratin 7/17 Antibody (C-46) - BSA Free [NB500-352]

Flow Cytometry: Cytokeratin 7/17 Antibody (C-46) [NB500-352] - An intracellular stain was performed on HeLa cells with Cytokeratin 7/17 antibody (C-46) NB500-352PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
Flow Cytometry: Cytokeratin 7/17 Antibody (C-46) - BSA Free [NB500-352]

Flow Cytometry: Cytokeratin 7/17 Antibody (C-46) - BSA Free [NB500-352]

Flow Cytometry: Cytokeratin 7/17 Antibody (C-46) [NB500-352] - Using the Alexa Fluor 488 direct conjugate An intracellular stain was performed on A549 cells with Cytokeratin 7/17 (C-46) antibody NB500-352AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Applications for Cytokeratin 7/17 Antibody (C-46) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

10 ug/ml

Immunoprecipitation

1:50

Western Blot

1-2 ug/ml
Application Notes
Western Blot - Sample preparation: Lysis 1% lauryl maltoside for 1 hour, 5x10 sec. sonication; 5 min. in hot Laemmli buffer (reducing conditions.) Immunohistochemistry-Paraffin sections - Pretreatment: 0,1% pepsin, 30 min, 37C Incubation: 4C overnight Application note: chromogen DAB. This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cytokeratin 7/17

The protein encoded by the Cytokeratin 7 / 17 gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. (provided by RefSeq)

Alternate Names

CK7, CK-7, cytokeratin 7, Cytokeratin-7, K2C7, K7keratin, 55K type II cytoskeletal, keratin 7, keratin, type II cytoskeletal 7, Keratin-7, MGC129731, MGC3625, sarcolectin, SCLkeratin, simple epithelial type I, K7, type II mesothelial keratin K7, Type-II keratin Kb7

Entrez Gene IDs

3855 (Human); 110310 (Mouse); 300242 (Rat)

Gene Symbol

KRT7

Additional Cytokeratin 7/17 Products

Product Documents for Cytokeratin 7/17 Antibody (C-46) - BSA Free

Certificate of Analysis

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Product Specific Notices for Cytokeratin 7/17 Antibody (C-46) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cytokeratin 7/17 Antibody (C-46) - BSA Free

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Protocols

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