Cytokeratin 7 Antibody (OV-TL12/30)

Novus Biologicals | Catalog # NBP2-44814

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # OV-TL12/30
View all Cytokeratin 7 Primary Antibodies »

Product Specifications

Immunogen

OTN 11, ovarian carcinoma cell line

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30522949).

Localization

Cytoplasmic

Marker

Glandular and Transitional Epithelial Marker

Specificity

It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This monoclonal antibody is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This monoclonal antibody is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47940)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin 7 Antibody (OV-TL12/30)

Western Blot: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814]

Western Blot: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814]

Western Blot: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Antibody (OV-TL12/30).
Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814]

Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Immunofluorescent staining of methanol-fixed HeLa cells. Cytokeratin 7 Antibody (OV-TL12/30) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Paraffin-embedded human salivary gland tissue. Image from verified customer review. Image using the Azide and BSA Free format of this antibody.
Flow Cytometry: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814]

Flow Cytometry: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814]

Flow Cytometry: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Flow Cytometric Analysis of HeLa cells using Cytokeratin 7 Antibody (OV-TL12/30) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Human Lung SCC stained with Cytokeratin 7 Monoclonal Antibody (OV-TL12/30)
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Human Ovarian Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (OV-TL12/30)
Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814]

Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814]

Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Simple Western lane view shows a specific band for Cytokeratin 7 in 0.1 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814]

Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814]

Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 7 antibody was used at 1 ug/ml dilution of HeLa lysates(s) respectively.
Cytokeratin 7 Antibody (OV-TL12/30)

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] -

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Formalin-fixed, paraffin-embedded human prsotatecarcinoma stained with Biotin-conjugated Cytokeratin 7 Mouse Monoclonal Antibody (OV-TL12/30).
Cytokeratin 7 Antibody (OV-TL12/30)

Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] -

Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Lane view shows a specific band for Cytokeratin 7 at 5 ug/mL in HeLa cell lysate. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 61 kDa. Image from an internal validation.

Applications for Cytokeratin 7 Antibody (OV-TL12/30)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Simple Western

1 ug/mL

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate, separated by Size, antibody dilution of 1 ug/mL, apparent MW was 56 kDa.
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30239759). Use in Immunohistochemistry reported in scientific literature (PMID: 30522949).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 7

Alternate Names

CK-7, CK7, K2C7, K7

Gene Symbol

KRT7

UniProt

P08729 (Human)

Additional Cytokeratin 7 Products

Product Documents for Cytokeratin 7 Antibody (OV-TL12/30)

Certificate of Analysis

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Product Specific Notices for Cytokeratin 7 Antibody (OV-TL12/30)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cytokeratin 7 Antibody (OV-TL12/30)

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Protocols

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