Cytokeratin, LMW Antibody (AE-1) - IHC-Prediluted

Novus Biologicals | Catalog # NBP2-48267

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken, Monkey, Rabbit, Reptile

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # AE-1

Format

IHC-Prediluted
View all Cytokeratin, LMW Primary Antibodies »

Product Specifications

Immunogen

Human epidermal keratin (Uniprot: Q7Z794)

Reactivity Notes

Turtle

Localization

Cytoplasmic

Marker

Epithelial Marker

Specificity

This monoclonal antibody recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI, 48, 46, 45, and 40kDa. monoclonal antibody AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for Cytokeratin, LMW Antibody (AE-1) - IHC-Prediluted

Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (AE-1) - IHC-Prediluted [NBP2-48267]

Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (AE-1) - IHC-Prediluted [NBP2-48267]

Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (AE-1) - IHC-Prediluted [NBP2-48267] - Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, LMW Mouse Monoclonal Antibody (AE-1).
Cytokeratin, LMW Antibody (AE-1) - IHC-Prediluted Western Blot: Cytokeratin, LMW Antibody (AE-1) - IHC-Prediluted [NBP2-48267] -

Western Blot: Cytokeratin, LMW Antibody (AE-1) - IHC-Prediluted [NBP2-48267] -

Western Blot Analysis of A431 cells using Cytokeratin, LMW Antibody (AE-1) - IHC-Prediluted.

Applications for Cytokeratin, LMW Antibody (AE-1) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin, LMW

Alternate Names

Cytokeratin LMW, cytokeratin-1B, keratin 77, keratin, type II cytoskeletal 1b, KRT77, type-II keratin Kb39

Gene Symbol

KRT77

Additional Cytokeratin, LMW Products

Product Documents for Cytokeratin, LMW Antibody (AE-1) - IHC-Prediluted

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin, LMW Antibody (AE-1) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Cytokeratin, LMW Antibody (AE-1) - IHC-Prediluted

There are currently no reviews for this product. Be the first to review Cytokeratin, LMW Antibody (AE-1) - IHC-Prediluted and earn rewards!

Have you used Cytokeratin, LMW Antibody (AE-1) - IHC-Prediluted?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies